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HRP-Biotin Conjugate

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H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
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Biotinylated horse radish peroxidase (HRP) is an excellent tool for biological detections with ELISA and other enzyme-coupled assays. Streptavidin selectively binds to biotin and the conjugated HRP provides enzyme activity for detection using an appropriate HRP substrate system via color, fluorescence, or luminescence. This product has been used primarily in ELISA applications to provide consistent measurement of streptavidin-conjugated detection antibodies.

Example protocol


Concentration: 5 mg/mL
Formulation: 1 mL in 1X PBS
Stabilizer: 1% bovine serum albumin and 0.01% thimerosal
Expiration Date: 12 months upon receiving


Streptavidin-HRP solution
To prepare 25 mL of Streptavidin-HRP solution for ELISA, mix the following reagents:
1x PBS with 0.02% Tween-20: 25 mL
Biotinylated peroxidase (5 mg/mL):  1 µL
Streptavidin (5 mg/mL): 2 µL
Incubate the mix for 30 minutes at room temperature before use.
Note     Discard unused diluted complex after 24 hours.



View all 50 references: Citation Explorer
Antibody-biotin-streptavidin-horseradish peroxidase (HRP) sensor for rapid and ultra-sensitive detection of fumonisins.
Authors: Yang, Hualin and Zhang, Qi and Liu, Xiaolei and Yang, Yuying and Yang, Yong and Liu, Mingyuan and Li, Peiwu and Zhou, Yu
Journal: Food chemistry (2020): 126356
Competitive HRP-Linked Colorimetric Aptasensor for the Detection of Fumonisin B1 in Food based on Dual Biotin-Streptavidin Interaction.
Authors: Tao, Zui and Zhou, You and Li, Xiang and Wang, Zhouping
Journal: Biosensors (2020)
An Electrochemical Sense Array Based on Aptamer and Biotin-Avidin System for the Selective Detection of Glucagon-Like Peptide-1.
Authors: Wu, Jie and He, Tianmin and Guo, Pingfan and Cai, Fanggang and Zhao, Chengfei
Journal: Clinical laboratory (2019)
Enzyme immobilization on protein-resistant PNIPAAm brushes: impact of biotin linker length on enzyme amount and catalytic activity.
Authors: Rosenthal, Alice and Rauch, Sebastian and Eichhorn, Klaus-Jochen and Stamm, Manfred and Uhlmann, Petra
Journal: Colloids and surfaces. B, Biointerfaces (2018): 351-357
A Biotin-streptavidin-enhanced Carbon Nanotube Amplification Strategy for an Ultrasensitive Immunodetection of Polybrominated Diphenyl Ethers.
Authors: Zhang, Xiaohan and Zhuang, Huisheng
Journal: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry (2017): 1441-1446
Development of a progesterone immunosensor based on thionine-graphene oxide composites platforms: Improvement by biotin-streptavidin-amplified system.
Authors: Dong, Xiu-Xiu and Yuan, Li-Peng and Liu, Yi-Xin and Wu, Min-Fu and Liu, Bo and Sun, Yuan-Ming and Shen, Yu-Dong and Xu, Zhen-Lin
Journal: Talanta (2017): 502-508
Streptavidin-biotin-peroxidase nanocomplex-amplified microfluidics immunoassays for simultaneous detection of inflammatory biomarkers.
Authors: Wu, Jing and Chen, Yiping and Yang, Mingzhu and Wang, Yu and Zhang, Cheng and Yang, Mo and Sun, Jiashu and Xie, Mengxia and Jiang, Xingyu
Journal: Analytica chimica acta (2017): 138-147
Highly Sensitive Two-Dimensional Paper Network Incorporating Biotin-Streptavidin for the Detection of Malaria.
Authors: Grant, Benjamin D and Smith, Chelsey A and Karvonen, Kristine and Richards-Kortum, Rebecca
Journal: Analytical chemistry (2016): 2553-7
Biotin-Streptavidin Competition Mediates Sensitive Detection of Biomolecules in Enzyme Linked Immunosorbent Assay.
Authors: Lakshmipriya, Thangavel and Gopinath, Subash C B and Tang, Thean-Hock
Journal: PloS one (2016): e0151153
Electrochemical biotin detection based on magnetic beads and a new magnetic flow cell for screen printed electrode.
Authors: Biscay, Julien and González García, María Begoña and Costa García, Agustín
Journal: Talanta (2015): 706-11