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HRP-Biotin Conjugate
Biotinylated horse radish peroxidase (HRP) is an excellent tool for biological detections with ELISA and other enzyme-coupled assays. Streptavidin selectively binds to biotin and the conjugated HRP provides enzyme activity for detection using an appropriate HRP substrate system via color, fluorescence, or luminescence. This product has been used primarily in ELISA applications to provide consistent measurement of streptavidin-conjugated detection antibodies.
Example protocol
AT A GLANCE
Concentration: 5 mg/mL
Formulation: 1 mL in 1X PBS
Stabilizer: 1% bovine serum albumin and 0.01% thimerosal
Expiration Date: 12 months upon receiving
Formulation: 1 mL in 1X PBS
Stabilizer: 1% bovine serum albumin and 0.01% thimerosal
Expiration Date: 12 months upon receiving
PREPARATION OF WORKING SOLUTION
Streptavidin-HRP solution
To prepare 25 mL of Streptavidin-HRP solution for ELISA, mix the following reagents:1x PBS with 0.02% Tween-20: 25 mL
Biotinylated peroxidase (5 mg/mL): 1 µL
Streptavidin (5 mg/mL): 2 µL
Incubate the mix for 30 minutes at room temperature before use.
Note Discard unused diluted complex after 24 hours.
References
View all 50 references: Citation Explorer
Antibody-biotin-streptavidin-horseradish peroxidase (HRP) sensor for rapid and ultra-sensitive detection of fumonisins.
Authors: Yang, Hualin and Zhang, Qi and Liu, Xiaolei and Yang, Yuying and Yang, Yong and Liu, Mingyuan and Li, Peiwu and Zhou, Yu
Journal: Food chemistry (2020): 126356
Authors: Yang, Hualin and Zhang, Qi and Liu, Xiaolei and Yang, Yuying and Yang, Yong and Liu, Mingyuan and Li, Peiwu and Zhou, Yu
Journal: Food chemistry (2020): 126356
Competitive HRP-Linked Colorimetric Aptasensor for the Detection of Fumonisin B1 in Food based on Dual Biotin-Streptavidin Interaction.
Authors: Tao, Zui and Zhou, You and Li, Xiang and Wang, Zhouping
Journal: Biosensors (2020)
Authors: Tao, Zui and Zhou, You and Li, Xiang and Wang, Zhouping
Journal: Biosensors (2020)
An Electrochemical Sense Array Based on Aptamer and Biotin-Avidin System for the Selective Detection of Glucagon-Like Peptide-1.
Authors: Wu, Jie and He, Tianmin and Guo, Pingfan and Cai, Fanggang and Zhao, Chengfei
Journal: Clinical laboratory (2019)
Authors: Wu, Jie and He, Tianmin and Guo, Pingfan and Cai, Fanggang and Zhao, Chengfei
Journal: Clinical laboratory (2019)
Enzyme immobilization on protein-resistant PNIPAAm brushes: impact of biotin linker length on enzyme amount and catalytic activity.
Authors: Rosenthal, Alice and Rauch, Sebastian and Eichhorn, Klaus-Jochen and Stamm, Manfred and Uhlmann, Petra
Journal: Colloids and surfaces. B, Biointerfaces (2018): 351-357
Authors: Rosenthal, Alice and Rauch, Sebastian and Eichhorn, Klaus-Jochen and Stamm, Manfred and Uhlmann, Petra
Journal: Colloids and surfaces. B, Biointerfaces (2018): 351-357
A Biotin-streptavidin-enhanced Carbon Nanotube Amplification Strategy for an Ultrasensitive Immunodetection of Polybrominated Diphenyl Ethers.
Authors: Zhang, Xiaohan and Zhuang, Huisheng
Journal: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry (2017): 1441-1446
Authors: Zhang, Xiaohan and Zhuang, Huisheng
Journal: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry (2017): 1441-1446
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