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ICG azide

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Physical properties
Molecular weight799.05
SolventDMSO
Spectral properties
Correction Factor (280 nm)0.076
Extinction coefficient (cm -1 M -1)230000
Excitation (nm)789
Emission (nm)813
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Direct upgrades
iFluor® 790 Azide

OverviewpdfSDSpdfProtocol


Molecular weight
799.05
Correction Factor (280 nm)
0.076
Extinction coefficient (cm -1 M -1)
230000
Excitation (nm)
789
Emission (nm)
813
Indocyanine green (ICG) is a tricarbocyanine-type dye with NIR-absorbing properties (peak absorption around 780 nm) and emission maximum at ~800 nm. This dye is also called Cardio Green and a few other less common trade names. The non-invasive near-infrared (NIR) fluorescence imaging dye ICG is approved by the FDA for ophthalmologic angiography to determine cardiac output and liver blood flow and function. Since infrared frequencies penetrate retinal layers, allowing ICG angiography to image deeper patterns of circulation than fluorescein angiography. ICG binds tightly to plasma proteins and becomes confined to the vascular system. ICG has a half-life of 150 to 180 seconds and is removed from circulation exclusively by the liver to bile juice. A recent study indicated ICG targets atheromas within 20 min of injection and provides sufficient signal enhancement for in vivo detection of lipid-rich, inflamed, coronary-sized plaques in atherosclerotic rabbits. Ex vivo fluorescence reflectance imaging showed high plaque target-to-background ratios in atheroma-bearing rabbits injected with ICG compared to atheroma-bearing rabbits injected with saline. It is also used in other medical diagnostics and cancer patients for the detection of solid tumors, localization of lymphnodes, and for angiography during reconstructive surgery, visualization of retinal and choroidal vasculature, and photodynamic therapy. In cancer diagnostics and therapeutics, ICG could be used as both an imaging dye and a hyperthermia agent. Little absorption in the visible range accounts for the low autofluorescence, tissue absorbance, and scattering at NIR wavelengths (700-900 nm). This ICG azide can be used to label alkyne-tagged biomolecules (like proteins, lipids, nucleic acids, sugars) chemoselectively via the well known click-chemistry.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of ICG azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM125.149 µL625.743 µL1.251 mL6.257 mL12.515 mL
5 mM25.03 µL125.149 µL250.297 µL1.251 mL2.503 mL
10 mM12.515 µL62.574 µL125.149 µL625.743 µL1.251 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Correction Factor (280 nm)0.076
Extinction coefficient (cm -1 M -1)230000
Excitation (nm)789
Emission (nm)813

Images


Citations


View all 5 citations: Citation Explorer
Comparison of Near-Infrared Imaging Agents Targeting the PTPmu Tumor Biomarker
Authors: Johansen, Mette L and Vincent, Jason and Rose, Marissa and Sloan, Andrew E and Brady-Kalnay, Susann M
Journal: Molecular Imaging and Biology (2023): 1--14
Assessment of Lexiscan for Blood Brain Barrier disruption to facilitate Fluorescence brain imaging
Authors: Pak, Rebecca W and Le, Hanh and Valentine, Heather and Thorek, Daniel and Rahmim, Arman and Wong, Dean and Kang, Jin U
Journal: (2017): ATu3B--2
Bioengineering of injectable encapsulated aggregates of pluripotent stem cells for therapy of myocardial infarction
Authors: Zhao, Shuting and Xu, Zhaobin and Wang, Hai and Reese, Benjamin E and Gushchina, Liubov V and Jiang, Meng and Agarwal, Pranay and Xu, Jiangsheng and Zhang, Mingjun and Shen, Rulong and others, undefined
Journal: Nature Communications (2016): 13306
Single-Layer MoS2 Nanosheets with Amplified Photoacoustic Effect for Highly Sensitive Photoacoustic Imaging of Orthotopic Brain Tumors
Authors: Chen, Jingqin and Liu, Chengbo and Hu, Dehong and Wang, Feng and Wu, Haiwei and Gong, Xiaojing and Liu, Xin and Song, Liang and Sheng, Zonghai and Zheng, Hairong
Journal: Advanced Functional Materials (2016)
Deep Photoacoustic/Luminescence/Magnetic Resonance Multimodal Imaging in Living Subjects Using High-Efficiency Upconversion Nanocomposites
Authors: Liu, Yu and Kang, Ning and Lv, Jing and Zhou, Zijian and Zhao, Qingliang and Ma, Lingceng and Chen, Zhong and Ren, Lei and Nie, Liming
Journal: Advanced Materials (2016)

References


View all 193 references: Citation Explorer
Sentinel lymph node biopsy using intraoperative indocyanine green fluorescence imaging navigated with preoperative CT lymphography for superficial esophageal cancer
Authors: Yuasa Y, Seike J, Yoshida T, Takechi H, Yamai H, Yamamoto Y, Furukita Y, Goto M, Minato T, Nishino T, Inoue S, Fujiwara S, Tangoku A.
Journal: Ann Surg Oncol (2012): 486
Indocyanine green angiography-guided photodynamic therapy for treatment of chronic central serous chorioretinopathy: a pilot study
Authors: Yannuzzi LA, Slakter JS, Gross NE, Spaide RF, Costa DL, Huang SJ, Klancnik JM, Jr., Aizman A.
Journal: Retina (2012): 288
Using indocyanine green fluorescent lymphography and lymphatic-venous anastomosis for cancer-related lymphedema
Authors: Mihara M, Murai N, Hayashi Y, Hara H, Iida T, Narushima M, Todokoro T, Uchida G, Yamamoto T, Koshima I.
Journal: Ann Vasc Surg (2012): 278 e1
Management of peripheral polypoidal choroidal vasculopathy with intravitreal bevacizumab and indocyanine green angiography-guided laser photocoagulation
Authors: Rishi P, Das A, Sarate P, Rishi E.
Journal: Indian J Ophthalmol (2012): 60
Synthesis and characterization of bovine serum albumin-coated nanocapsules loaded with indocyanine green as potential multifunctional nanoconstructs
Authors: Jung B, Anvari B.
Journal: Biotechnol Prog (2012): 533
Near-infrared autofluorescence and indocyanine green angiography in central serous chorioretinopathy
Authors: Lindner E, Weinberger A, Kirschkamp T, El-Shabrawi Y, Barounig A.
Journal: Ophthalmologica (2012): 34
Imaging and evaluation of corneal vascularization using fluorescein and indocyanine green angiography
Authors: Anijeet DR, Zheng Y, Tey A, Hodson M, Sueke H, Kaye SB.
Journal: Invest Ophthalmol Vis Sci (2012): 650
Indocyanine green fluorescence-guided sentinel lymph node biopsy in dermato-oncology
Authors: Stoffels I, von der Stuck H, Boy C, Poppel T, Korber N, Weindorf M, Dissemond J, Schadendorf D, Klode J.
Journal: J Dtsch Dermatol Ges (2012): 51
Establishment of novel detection system for embryonic stem cell-derived hepatocyte-like cells based on nongenetic manipulation with indocyanine green
Authors: Yoshie S, Ito J, Shirasawa S, Yokoyama T, Fujimura Y, Takeda K, Mizuguchi M, Matsumoto K, Tomotsune D, Sasaki K.
Journal: Tissue Eng Part C Methods (2012): 12
A simple and effective technique for identification of intersegmental planes by infrared thoracoscopy after transbronchial injection of indocyanine green
Authors: Sekine Y, Ko E, Oishi H, Miwa M.
Journal: J Thorac Cardiovasc Surg. (2012)