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ICG azide

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Physical properties
Molecular weight799.05
SolventDMSO
Spectral properties
Correction Factor (280 nm)0.076
Extinction coefficient (cm -1 M -1)230000
Excitation (nm)789
Emission (nm)813
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Direct upgrades
iFluor™ 790 Azide

OverviewpdfSDSpdfProtocol


Molecular weight
799.05
Correction Factor (280 nm)
0.076
Extinction coefficient (cm -1 M -1)
230000
Excitation (nm)
789
Emission (nm)
813
Indocyanine green (ICG) is a tricarbocyanine-type dye with NIR-absorbing properties (peak absorption around 780 nm) and emission maximum at ~800 nm. This dye is also called Cardio Green and a few other less common trade names. The non-invasive near-infrared (NIR) fluorescence imaging dye ICG is approved by the FDA for ophthalmologic angiography to determine cardiac output and liver blood flow and function. Since infrared frequencies penetrate retinal layers, allowing ICG angiography to image deeper patterns of circulation than fluorescein angiography. ICG binds tightly to plasma proteins and becomes confined to the vascular system. ICG has a half-life of 150 to 180 seconds and is removed from circulation exclusively by the liver to bile juice. A recent study indicated ICG targets atheromas within 20 min of injection and provides sufficient signal enhancement for in vivo detection of lipid-rich, inflamed, coronary-sized plaques in atherosclerotic rabbits. Ex vivo fluorescence reflectance imaging showed high plaque target-to-background ratios in atheroma-bearing rabbits injected with ICG compared to atheroma-bearing rabbits injected with saline. It is also used in other medical diagnostics and cancer patients for the detection of solid tumors, localization of lymphnodes, and for angiography during reconstructive surgery, visualization of retinal and choroidal vasculature, and photodynamic therapy. In cancer diagnostics and therapeutics, ICG could be used as both an imaging dye and a hyperthermia agent. Little absorption in the visible range accounts for the low autofluorescence, tissue absorbance, and scattering at NIR wavelengths (700-900 nm). This ICG azide can be used to label alkyne-tagged biomolecules (like proteins, lipids, nucleic acids, sugars) chemoselectively via the well known click-chemistry.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of ICG azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM125.149 µL625.743 µL1.251 mL6.257 mL12.515 mL
5 mM25.03 µL125.149 µL250.297 µL1.251 mL2.503 mL
10 mM12.515 µL62.574 µL125.149 µL625.743 µL1.251 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Spectrum


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spectrum

Spectral properties

Correction Factor (280 nm)0.076
Extinction coefficient (cm -1 M -1)230000
Excitation (nm)789
Emission (nm)813

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
ICG-ATT [3-ICG-acyl-1,3-thiazolidine-2-thione]7898132300000.076
ICG-OSu7898132300000.076
AMCA Azide346434190000.153
XFD488 azide *Same Structure to Alexa Fluor™ 488 azide*499520710000.11
Cy3B azide56057112000010.069
XFD647 Azide6506712700000.03
ICG-Osu *UltraPure Grade*7898132300000.076

Images


Citations


View all 5 citations: Citation Explorer
Comparison of Near-Infrared Imaging Agents Targeting the PTPmu Tumor Biomarker
Authors: Johansen, Mette L and Vincent, Jason and Rose, Marissa and Sloan, Andrew E and Brady-Kalnay, Susann M
Journal: Molecular Imaging and Biology (2023): 1--14
Assessment of Lexiscan for Blood Brain Barrier disruption to facilitate Fluorescence brain imaging
Authors: Pak, Rebecca W and Le, Hanh and Valentine, Heather and Thorek, Daniel and Rahmim, Arman and Wong, Dean and Kang, Jin U
Journal: (2017): ATu3B--2
Bioengineering of injectable encapsulated aggregates of pluripotent stem cells for therapy of myocardial infarction
Authors: Zhao, Shuting and Xu, Zhaobin and Wang, Hai and Reese, Benjamin E and Gushchina, Liubov V and Jiang, Meng and Agarwal, Pranay and Xu, Jiangsheng and Zhang, Mingjun and Shen, Rulong and others, undefined
Journal: Nature Communications (2016): 13306
Single-Layer MoS2 Nanosheets with Amplified Photoacoustic Effect for Highly Sensitive Photoacoustic Imaging of Orthotopic Brain Tumors
Authors: Chen, Jingqin and Liu, Chengbo and Hu, Dehong and Wang, Feng and Wu, Haiwei and Gong, Xiaojing and Liu, Xin and Song, Liang and Sheng, Zonghai and Zheng, Hairong
Journal: Advanced Functional Materials (2016)
Deep Photoacoustic/Luminescence/Magnetic Resonance Multimodal Imaging in Living Subjects Using High-Efficiency Upconversion Nanocomposites
Authors: Liu, Yu and Kang, Ning and Lv, Jing and Zhou, Zijian and Zhao, Qingliang and Ma, Lingceng and Chen, Zhong and Ren, Lei and Nie, Liming
Journal: Advanced Materials (2016)

References


View all 193 references: Citation Explorer
Ultrastaging of colon cancer by sentinel node biopsy using fluorescence navigation with indocyanine green
Authors: Hirche C, Mohr Z, Kneif S, Doniga S, Murawa D, Strik M, Hunerbein M.
Journal: Int J Colorectal Dis (2012): 319
Sentinel lymph node biopsy using real-time fluorescence navigation with indocyanine green in cutaneous head and neck/lip mucosa melanomas
Authors: Hayashi T, Furukawa H, Oyama A, Funayama E, Saito A, Yamao T, Yamamoto Y.
Journal: Head Neck (2012): 758
Efficacy of indocyanine green videography and real-time evaluation by FLOW 800 in the resection of a spinal cord hemangioblastoma in a child
Authors: Ueba T, Abe H, Matsumoto J, Higashi T, Inoue T.
Journal: J Neurosurg Pediatr (2012): 428
Concentration of indocyanine green does not significantly influence lymphatic function as assessed by near-infrared imaging
Authors: Aldrich MB, Davies-Venn C, Angermiller B, Robinson H, Chan W, Kwon S, Sevick-Muraca EM.
Journal: Lymphat Res Biol (2012): 20
Comparative Study of the Optical and Heat Generation Properties of IR820 and Indocyanine Green
Authors: Fern, undefined and ez-Fern, undefined and ez A, Manch and a R, Lei T, Carvajal DA, Tang Y, Zahid Raza Kazmi S, McGoron AJ.
Journal: Mol Imaging (2012): 99
Sentinel lymph node biopsy using a new indocyanine green fluorescence imaging system with a colour charged couple device camera for oral cancer
Authors: Iwai T, Maegawa J, Hirota M, Tohnai I.
Journal: Br J Oral Maxillofac Surg. (2012)
Usefulness of indocyanine green angiography to depict the distant retinal vascular anomalies associated with branch retinal vein occlusion causing serous macular detachment
Authors: Ueda T, Gomi F, Suzuki M, Sakaguchi H, Sawa M, Kamei M, Nishida K.
Journal: Retina (2012): 308
Application of indocyanine green videoangiography in surgery for spinal vascular malformations
Authors: Misra BK, Pur and are HR., undefined
Journal: J Clin Neurosci. (2012)
Indocyanine-Green Angiography Findings in Susac's Syndrome
Authors: Balaskas K, Guex-Crosier Y, Borruat FX.
Journal: Klin Monbl Augenheilkd (2012): 426
Gold nanomaterials conjugated with indocyanine green for dual-modality photodynamic and photothermal therapy
Authors: Kuo WS, Chang YT, Cho KC, Chiu KC, Lien CH, Yeh CS, Chen SJ.
Journal: Biomaterials (2012): 3270