ICG azide | AAT Bioquest

ICG azide

Indocyanine green (ICG) is a tricarbocyanine-type dye with NIR-absorbing properties (peak absorption around 780 nm) and emission maximum at ~800 nm. This dye is also called Cardio Green and a few other less common trade names. The non-invasive near-infrared (NIR) fluorescence imaging dye ICG is approved by the FDA for ophthalmologic angiography to determine cardiac output and liver blood flow and function. Since infrared frequencies penetrate retinal layers, allowing ICG angiography to image deeper patterns of circulation than fluorescein angiography. ICG binds tightly to plasma proteins and becomes confined to the vascular system. ICG has a half-life of 150 to 180 seconds and is removed from circulation exclusively by the liver to bile juice. A recent study indicated ICG targets atheromas within 20 min of injection and provides sufficient signal enhancement for in vivo detection of lipid-rich, inflamed, coronary-sized plaques in atherosclerotic rabbits. Ex vivo fluorescence reflectance imaging showed high plaque target-to-background ratios in atheroma-bearing rabbits injected with ICG compared to atheroma-bearing rabbits injected with saline. It is also used in other medical diagnostics and cancer patients for the detection of solid tumors, localization of lymphnodes, and for angiography during reconstructive surgery, visualization of retinal and choroidal vasculature, and photodynamic therapy. In cancer diagnostics and therapeutics, ICG could be used as both an imaging dye and a hyperthermia agent. Little absorption in the visible range accounts for the low autofluorescence, tissue absorbance, and scattering at NIR wavelengths (700-900 nm). This ICG azide can be used to label alkyne-tagged biomolecules (like proteins, lipids, nucleic acids, sugars) chemoselectively via the well known click-chemistry.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
985	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of ICG azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	125.149 µL	625.743 µL	1.251 mL	6.257 mL	12.515 mL
5 mM	25.03 µL	125.149 µL	250.297 µL	1.251 mL	2.503 mL
10 mM	12.515 µL	62.574 µL	125.149 µL	625.743 µL	1.251 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	799.05 g/mol	=	mL	x	mM	=	mmol

Citations

View all 5 citations: Citation Explorer

Comparison of Near-Infrared Imaging Agents Targeting the PTPmu Tumor Biomarker

Authors:

Johansen, Mette L and Vincent, Jason and Rose, Marissa and Sloan, Andrew E and Brady-Kalnay, Susann M

Journal:

Molecular Imaging and Biology (2023): 1--14

Assessment of Lexiscan for Blood Brain Barrier disruption to facilitate Fluorescence brain imaging

Authors:

Pak, Rebecca W and Le, Hanh and Valentine, Heather and Thorek, Daniel and Rahmim, Arman and Wong, Dean and Kang, Jin U

Bioengineering of injectable encapsulated aggregates of pluripotent stem cells for therapy of myocardial infarction

Authors:

Zhao, Shuting and Xu, Zhaobin and Wang, Hai and Reese, Benjamin E and Gushchina, Liubov V and Jiang, Meng and Agarwal, Pranay and Xu, Jiangsheng and Zhang, Mingjun and Shen, Rulong and others, undefined

Journal:

Nature Communications (2016): 13306

Single-Layer MoS2 Nanosheets with Amplified Photoacoustic Effect for Highly Sensitive Photoacoustic Imaging of Orthotopic Brain Tumors

Authors:

Chen, Jingqin and Liu, Chengbo and Hu, Dehong and Wang, Feng and Wu, Haiwei and Gong, Xiaojing and Liu, Xin and Song, Liang and Sheng, Zonghai and Zheng, Hairong

Journal:

Advanced Functional Materials (2016)

Deep Photoacoustic/Luminescence/Magnetic Resonance Multimodal Imaging in Living Subjects Using High-Efficiency Upconversion Nanocomposites

Authors:

Liu, Yu and Kang, Ning and Lv, Jing and Zhou, Zijian and Zhao, Qingliang and Ma, Lingceng and Chen, Zhong and Ren, Lei and Nie, Liming

Journal:

Advanced Materials (2016)

References

View all 193 references: Citation Explorer

Sentinel lymph node biopsy using intraoperative indocyanine green fluorescence imaging navigated with preoperative CT lymphography for superficial esophageal cancer

Authors:

Yuasa Y, Seike J, Yoshida T, Takechi H, Yamai H, Yamamoto Y, Furukita Y, Goto M, Minato T, Nishino T, Inoue S, Fujiwara S, Tangoku A.

Journal:

Ann Surg Oncol (2012): 486

Indocyanine green angiography-guided photodynamic therapy for treatment of chronic central serous chorioretinopathy: a pilot study

Authors:

Yannuzzi LA, Slakter JS, Gross NE, Spaide RF, Costa DL, Huang SJ, Klancnik JM, Jr., Aizman A.

Journal:

Retina (2012): 288

Using indocyanine green fluorescent lymphography and lymphatic-venous anastomosis for cancer-related lymphedema

Authors:

Mihara M, Murai N, Hayashi Y, Hara H, Iida T, Narushima M, Todokoro T, Uchida G, Yamamoto T, Koshima I.

Journal:

Ann Vasc Surg (2012): 278 e1

Management of peripheral polypoidal choroidal vasculopathy with intravitreal bevacizumab and indocyanine green angiography-guided laser photocoagulation

Authors:

Rishi P, Das A, Sarate P, Rishi E.

Journal:

Indian J Ophthalmol (2012): 60

Synthesis and characterization of bovine serum albumin-coated nanocapsules loaded with indocyanine green as potential multifunctional nanoconstructs

Authors:

Jung B, Anvari B.

Journal:

Biotechnol Prog (2012): 533

Physical properties

Molecular weight	799.05
Solvent	DMSO

Spectral properties

Correction factor (280 nm)	0.076
Extinction coefficient (cm ^-1 M ^-1)	230000
Excitation (nm)	789
Emission (nm)	813

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 7, 2025