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ICG-TFP ester

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Physical properties
Molecular weight981.06
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Indocyanine green (ICG) is a cyanine dye used in medical diagnostics. It is used for determining cardiac output, hepatic function, and liver blood flow, and for ophthalmic angiography. It has a peak spectral absorption close to 800 nm. These infrared frequencies penetrate retinal layers, allowing ICG angiography to image deeper patterns of circulation than fluorescein angiography. The amino-reactive ICG-Sulfo-OSu (#180) is predominantly used to make various ICG bioconjugates with antibodies and other biological molecules since it has improved water solubility compared to other ICG amine-reactive derivatives. However, the well-known ICG-Sulfo-OSu is extremely unstable. ICG-TFP ester has significantly improved stability and good water solubility. Under the same conditions, it gives higher conjugation yield than ICG-Sulfo-OSu.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of ICG-TFP ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM101.931 µL509.653 µL1.019 mL5.097 mL10.193 mL
5 mM20.386 µL101.931 µL203.861 µL1.019 mL2.039 mL
10 mM10.193 µL50.965 µL101.931 µL509.653 µL1.019 mL

Molarity calculator

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View all 5 citations: Citation Explorer
In vivo photoacoustic imaging of cancer using indocyanine green-labeled monoclonal antibody targeting the epidermal growth factor receptor
Authors: Sano, K., Ohashi, M., Kanazaki, K., Ding, N., Deguchi, J., Kanada, Y., Ono, M., Saji, H.
Journal: Biochem Biophys Res Commun (2015): 820-5
In vitro and in vivo analysis of indocyanine green-labeled panitumumab for optical imaging-a cautionary tale
Authors: Zhou, Y., Kim, Y. S., Milenic, D. E., Baidoo, K. E., Brechbiel, M. W.
Journal: Bioconjug Chem (2014): 1801-10
Short PEG-linkers improve the performance of targeted, activatable monoclonal antibody-indocyanine green optical imaging probes
Authors: Sano, K., Nakajima, T., Miyazaki, K., Ohuchi, Y., Ikegami, T., Choyke, P. L., Kobayashi, H.
Journal: Bioconjug Chem (2013): 811-6
Dual-modality imaging with 99mTc and fluorescent indocyanine green using surface-modified silica nanoparticles for biopsy of the sentinel lymph node: an animal study
Authors: Tsuchimochi, M., Hayama, K., Toyama, M., Sasagawa, I., Tsubokawa, N.
Journal: EJNMMI Res (2013): 33
Activatable fluorescent cys-diabody conjugated with indocyanine green derivative: consideration of fluorescent catabolite kinetics on molecular imaging
Authors: Sano, K., Nakajima, T., Ali, T., Bartlett, D. W., Wu, A. M., Kim, I., Paik, C. H., Choyke, P. L., Kobayashi, H.
Journal: J Biomed Opt (2013): 101304