AAT Bioquest

IDA succinimidyl ester

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InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight429.43
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
Alternative formats
IDA maleimide


Molecular weight
Immobilized metal affinity chromatography (IMAC) is a popular method for protein purification, particularly for recombinant proteins fused to a polyhistidine-tag. Transition metal ions immobilized to a matrix through a chelating ligand interact with the polyhistidine-tag, effectively sequestering the fused protein from a sample. Nitrilotriacetic acid (NTA) and iminodiacetic acid (IDA) are two such ligands commonly used in commercially available resins. AAT Bioquest offer a variety of NTA building blocks for developing either NTA-based purification and detection. IDA is complimentary to NTA. The tridentate IDA ligand requires a lower imidazole concentration to elute protein than the tetradentate NTA. IDA is a smaller molecule which can be coupled to the matrix at a higher density resulting in a higher metal loading capacity. IDA succinimidyl ester is an amino-reactive building block that can be used to develop a variety of IDA probes for detecting polyHis-containing proteins, and tools for purifying polyHis tagged proteins.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of IDA succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM232.867 µL1.164 mL2.329 mL11.643 mL23.287 mL
5 mM46.573 µL232.867 µL465.734 µL2.329 mL4.657 mL
10 mM23.287 µL116.433 µL232.867 µL1.164 mL2.329 mL

Molarity calculator

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View all 28 references: Citation Explorer
Heterologous overexpression of active hexokinases from microsporidia Nosema bombycis and Nosema ceranae confirms their ability to phosphorylate host glucose.
Authors: Dolgikh, Viacheslav V and Tsarev, Alexander A and Timofeev, Sergey A and Zhuravlyov, Vladimir S
Journal: Parasitology research (2019): 1511-1518
Systematic analysis of the expression, solubility and purification of a passenger protein in fusion with different tags.
Authors: Bernier, Sarah C and Cantin, Line and Salesse, Christian
Journal: Protein expression and purification (2018): 92-106
Production of a truncated recombinant HA1 for influenza A H9 subtype screening.
Authors: Tombari, Wafa and Ghram, Abdeljelil
Journal: Biologicals : journal of the International Association of Biological Standardization (2016): 546-555
Characterization of the Eimeria maxima sporozoite surface protein IMP1.
Authors: Jenkins, M C and Fetterer, R and Miska, K and Tuo, W and Kwok, O and Dubey, J P
Journal: Veterinary parasitology (2015): 146-52
Effects of cholesterol incorporation on the physicochemical, colloidal, and biological characteristics of pH-sensitive AB₂ miktoarm polymer-based polymersomes.
Authors: Yin, Haiqing and Kang, Han Chang and Huh, Kang Moo and Bae, You Han
Journal: Colloids and surfaces. B, Biointerfaces (2014): 128-37
Layer-by-layer polyelectrolyte deposition: a mechanism for forming biocomposite materials.
Authors: Tan, Yerpeng and Yildiz, Umit Hakan and Wei, Wei and Waite, J Herbert and Miserez, Ali
Journal: Biomacromolecules (2013): 1715-26
Construction of the coding sequence of the transcription variant 2 of the human Renalase gene and its expression in the prokaryotic system.
Authors: Fedchenko, Valerii I and Kaloshin, Alexei A and Mezhevikina, Lyudmila M and Buneeva, Olga A and Medvedev, Alexei E
Journal: International journal of molecular sciences (2013): 12764-79
Controlled delivery of recombinant adeno-associated virus serotype 2 using pH-sensitive poly(ethylene glycol)-poly-L-histidine hydrogels.
Authors: Zeng, Yi-Fang and Tseng, S-Ja and Kempson, Ivan M and Peng, Shu-Fen and Wu, Wen-Teng and Liu, Je-Ruei
Journal: Biomaterials (2012): 9239-45
Detection of biological macromolecules on a biochip dedicated to UV specific absorption.
Authors: Robin, Kristelle and Reverchon, Jean-Luc and Mugherli, Laurent and Fromant, Michel and Plateau, Pierre and Benisty, Henri
Journal: Biosensors & bioelectronics (2009): 1585-91
[Isolation, prokaryotic expression and activity analysis of thymidylate kinase (tmk) gene from Phytoplasma of wheat blue dwarf].
Authors: Li, Bei and Ji, Lingling and Wu, Yunfeng and Hao, Xing'an
Journal: Wei sheng wu xue bao = Acta microbiologica Sinica (2008): 739-44