IDA succinimidyl ester

Immobilized metal affinity chromatography (IMAC) is a popular method for protein purification, particularly for recombinant proteins fused to a polyhistidine-tag. Transition metal ions immobilized to a matrix through a chelating ligand interact with the polyhistidine-tag, effectively sequestering the fused protein from a sample. Nitrilotriacetic acid (NTA) and iminodiacetic acid (IDA) are two such ligands commonly used in commercially available resins. AAT Bioquest offer a variety of NTA building blocks for developing either NTA-based purification and detection. IDA is complimentary to NTA. The tridentate IDA ligand requires a lower imidazole concentration to elute protein than the tetradentate NTA. IDA is a smaller molecule which can be coupled to the matrix at a higher density resulting in a higher metal loading capacity. IDA succinimidyl ester is an amino-reactive building block that can be used to develop a variety of IDA probes for detecting polyHis-containing proteins, and tools for purifying polyHis tagged proteins.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
12632	5 mg	Price

References

View all 28 references: Citation Explorer

Heterologous overexpression of active hexokinases from microsporidia Nosema bombycis and Nosema ceranae confirms their ability to phosphorylate host glucose.

Authors:

Dolgikh, Viacheslav V and Tsarev, Alexander A and Timofeev, Sergey A and Zhuravlyov, Vladimir S

Journal:

Parasitology research (2019): 1511-1518

Systematic analysis of the expression, solubility and purification of a passenger protein in fusion with different tags.

Authors:

Bernier, Sarah C and Cantin, Line and Salesse, Christian

Journal:

Protein expression and purification (2018): 92-106

Production of a truncated recombinant HA1 for influenza A H9 subtype screening.

Authors:

Tombari, Wafa and Ghram, Abdeljelil

Journal:

Biologicals : journal of the International Association of Biological Standardization (2016): 546-555

Characterization of the Eimeria maxima sporozoite surface protein IMP1.

Authors:

Jenkins, M C and Fetterer, R and Miska, K and Tuo, W and Kwok, O and Dubey, J P

Journal:

Veterinary parasitology (2015): 146-52

Effects of cholesterol incorporation on the physicochemical, colloidal, and biological characteristics of pH-sensitive AB₂ miktoarm polymer-based polymersomes.

Authors:

Yin, Haiqing and Kang, Han Chang and Huh, Kang Moo and Bae, You Han

Journal:

Colloids and surfaces. B, Biointerfaces (2014): 128-37

Physical properties

Molecular weight	429.43
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 2, 2025