iFluor® 350-dUTP *1 mM in TE Buffer (pH 7.5)*
Dye-modified deoxyuridine 5'-triphosphates are widely used for the production of dye-labeled DNA via conventional enzymatic incorporation techniques, including reverse transcription, nick translation, random primed labeling, and PCR. These enzymatic fluorescence labeling methods are commonly employed in both fluorescence in situ hybridization (FISH) probes and microarray-based experiments. The iFluor® 350-dUTP conjugate, which emits blue fluorescence, is compatible with the SpectrumBlue™ filter set (SpectrumBlue™ is a trademark of Vysis). Compared to commonly used blue fluorescent dNTPs such as AMCA-6-dUTP, iFluor® 350 provides a significantly brighter signal, greater photostability, and is less affected by pH variations.
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 350 maleimide | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
iFluor® 350 amine | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
iFluor® 350 hydrazide | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
iFluor® 350 Styramide *Superior Replacement for Alexa Fluor 350 tyramide* | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
iFluor® 350 Tyramide | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
iFluor® 440-dUTP *1 mM in TE Buffer (pH 7.5)* | 434 | 480 | 400001 | 0.671 | 0.352 | 0.229 |
iFluor®488-dUTP *1 mM in TE Buffer (pH 7.5)* | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 560-dUTP *1 mM in TE Buffer (pH 7.5)* | 560 | 571 | 1200001 | 0.571 | 0.0482 | 0.069 |
iFluor® 350 acid | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
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References
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Page updated on October 12, 2024