Name: iFluor® 350-dUTP *1 mM in TE Buffer (pH 7.5)*
Brand: AAT Bioquest
SKU: 17045
Price: 107 USD
Availability: InStock

iFluor® 350-dUTP

1 mM in TE Buffer (pH 7.5)

iFluor® 350-dUTP is a blue-fluorescent nucleotide analog for enzymatic incorporation into DNA, enabling the preparation of fluorescently labeled probes for nucleic acid detection and hybridization applications.

Enzymatic DNA Labeling: Enables fluorescent nucleotide incorporation into DNA during enzymatic synthesis.
Spectral Properties: Excitation at 345 nm and emission at 450 nm.
Labeling Compatibility: Supports PCR, nick translation, random priming, and reverse transcription.
Fluorescence Performance: Bright, photostable fluorescence compatible with SpectrumBlue™ filter sets.
Applications: Suitable for FISH probe preparation and microarray analysis.

Datasheet

SDS

Catalog	Size	Price	Quantity
17045	25 nmoles	Price

Overview

Dye-modified deoxyuridine 5'-triphosphates are widely used for the production of dye-labeled DNA via conventional enzymatic incorporation techniques, including reverse transcription, nick translation, random primed labeling, and PCR. These enzymatic fluorescence labeling methods are commonly employed in both fluorescence in situ hybridization (FISH) probes and microarray-based experiments. The iFluor® 350-dUTP conjugate, which emits blue fluorescence, is compatible with the SpectrumBlue™ filter set (SpectrumBlue™ is a trademark of Vysis). Compared to commonly used blue fluorescent dNTPs such as AMCA-6-dUTP, iFluor® 350 provides a significantly brighter signal, greater photostability, and is less affected by pH variations.

References

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Lanthanide Complex for Single-Molecule Fluorescent in Situ Hybridization and Background-Free Imaging.

Authors:

Su, Fei and Chen, Shiyu and Liu, Yuanhua and Zhou, Jiajia and Du, Zhongbo and Luo, Xiongjian and Wen, Shihui and Jin, Dayong

Journal:

Analytical chemistry (2024): 4430-4436

CircHIPK3 promotes bone microvascular endothelial cell proliferation, migration and angiogenesis by targeting miR-7 and KLF4/VEGF signaling in steroid-induced osteonecrosis of the femoral head.

Authors:

Peng, Peng and He, Wei and Zhang, Yi-Xi and Liu, Xiao-Hua and Chen, Zhen-Qiu and Mao, Ji-Gang

Journal:

Advances in clinical and experimental medicine : official organ Wroclaw Medical University (2023): 43-55

The Long Noncoding RNA Gm9866/Nuclear Factor-κB Axis Promotes Macrophage Polarization.

Authors:

Liao, Xiaomin and Ruan, Xianxian and Chen, Xiubing and Zheng, Biaolin and Wu, Xianbin and Deng, Zhejun and Yang, Zhe and Wang, Feng and Qin, Shanyu and Jiang, Haixing

Journal:

Mediators of inflammation (2023): 9991916

Inhibition of PLA2G4E/cPLA2 promotes survival of random skin flaps by alleviating Lysosomal membrane permeabilization-Induced necroptosis.

Authors:

Lou, Junsheng and Wang, Xiangyang and Zhang, Haojie and Yu, Gaoxiang and Ding, Jian and Zhu, Xuwei and Li, Yao and Wu, Yaosen and Xu, Hui and Xu, Huazi and Gao, Weiyang and Xiao, Jian and Zhou, Kailiang

Journal:

Autophagy (2022): 1841-1863

LINC01234 Accelerates the Progression of Breast Cancer via the miR-525-5p/Cold Shock Domain-Containing E1 Axis.

Authors:

Yu, Jia and Zhang, Xinxin and He, Rongfang and Yang, Xiaoyan and Hu, Juan and Tan, Fang and Yao, Jinyu and Lei, Xiaoyong and Wen, Gebo

Journal:

Disease markers (2022): 6899777

Physical properties

Molecular weight	1056.79
Solvent	Water

Spectral properties

Correction factor (260 nm)	0.83
Correction factor (280 nm)	0.23
Extinction coefficient (cm ^-1 M ^-1)	20000 ¹
Excitation (nm)	345
Emission (nm)	450
Quantum yield	0.95 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 24, 2026