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iFluor® 350-streptavidin conjugate
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules since streptavidin has a very high binding affinity for biotin. A variety of complementary biotinylated reagents are available from numerous commercial vendors. This iFluor® 350-streptavidin conjugate comprises streptavidin (the biotin-binding protein) with iFluor® 350 covalently attached (as the fluorescent label). It is commonly used as a second-step reagent for indirect immunofluorescent staining in conjunction with biotinylated primary antibodies. iFluor® 350-streptavidin conjugate has fluorescence excitation and emission maxima of ~345 nm and ~450 nm respectively. These spectral characteristics make it an excellent alternative to Alexa Fluor® 350-streptavidin conjugate (Alexa Fluor® is the trademark of Invitrogen). It is a very valuable tool for biotin-streptavidin-based biological assays and tests.
<p>HeLa cells were incubated with mouse anti-tubulin and biotin goat anti-mouse IgG followed by AAT&rsquo;s iFluor® 350-streptavidin conjugate.</p>
<p>HeLa cells were incubated with mouse anti-tubulin and biotin goat anti-mouse IgG followed by AAT&rsquo;s iFluor® 350-streptavidin conjugate.</p>
CatalogSize
Price
Quantity
16950200 ug
Price
169801 mg
Price
 
Physical properties

Molecular weight~52000
SolventWater
Spectral properties

Correction factor (260 nm)0.83
Correction factor (280 nm)0.23
Extinction coefficient (cm -1 M -1)
20000
1
Excitation (nm)345
Emission (nm)450
Quantum yield
0.95
1
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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Page updated on October 8, 2025