iFluor® 350-Wheat Germ Agglutinin (WGA) Conjugate
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Solvent | Water |
Correction Factor (260 nm) | 0.83 |
Correction Factor (280 nm) | 0.23 |
Extinction coefficient (cm -1 M -1) | 200001 |
Excitation (nm) | 345 |
Emission (nm) | 450 |
Quantum yield | 0.951 |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
Correction Factor (260 nm) 0.83 | Correction Factor (280 nm) 0.23 | Extinction coefficient (cm -1 M -1) 200001 | Excitation (nm) 345 | Emission (nm) 450 | Quantum yield 0.951 |
Platform
Fluorescence microscope
Excitation | DAPI filter set |
Emission | DAPI filter set |
Recommended plate | Black wall/clear bottom |
Example protocol
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles
Add 500 µL of ddH2O into the powder form to make a 2 mg/mL stock solution.
Note: The reconstituted conjugate solution can be stored at 2-8 °C for short-term storage or at -20 °C for long-term storage.
PREPARATION OF WORKING SOLUTION
Add 5 µL of 200X WGA conjugate solution to 1 mL HHBS Buffer.
Note: The optimized staining concentration may be different with different cell lines. The recommended starting concentration is 5-10 µg/mL for live cells.
SAMPLE EXPERIMENTAL PROTOCOL
Warm the vial to room temperature centrifuge briefly before opening. Staining protocols vary with applications. Appropriate dilution of conjugates should be determined experimentally.
- Wash cells twice with a HHBS buffer.
Add 100 µL iFluor® 350-WGA working solution.
- Incubate cells with WGA working solution for 10-30 minutes at 37 °C.
- Wash cells twice with HHBS buffer.
Image cells on a fluorescence microscope using DAPI filter set.
WGA conjugates can be also used to stain fixed cells.
Fix cells with 4% Formaldehyde in PBS.
Note: For fixed cell membrane staining, it is recommended to stain without the permeabilization step. A permeabilization step after fixation can facilitate staining intracellular compartments such as Golgi and Endoplasmic Reticulum (ER) structures.
Add 100 µL iFluor® 350-WGA working solution.
- Incubate cells with WGA working solution for 10-30 minutes at room temperature.
- Wash cells twice with HHBS buffer.
Image cells on a fluorescence microscope using DAPI filter set.
Spectrum

Spectral properties
Correction Factor (260 nm) | 0.83 |
Correction Factor (280 nm) | 0.23 |
Extinction coefficient (cm -1 M -1) | 200001 |
Excitation (nm) | 345 |
Emission (nm) | 450 |
Quantum yield | 0.951 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 488-Wheat Germ Agglutinin (WGA) Conjugate | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 555-Wheat Germ Agglutinin (WGA) Conjugate | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 594-Wheat Germ Agglutinin (WGA) Conjugate | 588 | 604 | 1800001 | 0.531 | 0.05 | 0.04 |
iFluor® 647-Wheat Germ Agglutinin (WGA) Conjugate | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
iFluor® 532-Wheat Germ Agglutinin (WGA) Conjugate | 537 | 560 | 900001 | 0.681 | 0.26 | 0.16 |
iFluor® 680-Wheat Germ Agglutinin (WGA) Conjugate | 684 | 701 | 2200001 | 0.231 | 0.097 | 0.094 |
iFluor® 700-Wheat Germ Agglutinin (WGA) Conjugate | 690 | 713 | 2200001 | 0.231 | 0.09 | 0.04 |
iFluor® 750-Wheat Germ Agglutinin (WGA) Conjugate | 757 | 779 | 2750001 | 0.121 | 0.044 | 0.039 |
iFluor® 790-Wheat Germ Agglutinin (WGA) Conjugate | 787 | 812 | 2500001 | 0.131 | 0.1 | 0.09 |
Images
References
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