Name: iFluor® 430 Tyramide
Brand: AAT Bioquest
SKU: 45096
Price: 329 USD
Availability: InStock

iFluor® 430 Tyramide

iFluor® 430 tyramide is optimized to a superior replacement for Opal 480 or other spectrally similar fluorescent tyramide conjugates or TSA reagents.

Detect extremely low-abundance targets in cells and tissues

<strong>Superior sensitivity with iFluor® 430 tyramide.</strong> HeLa cells were incubated with primary anti-tubulin antibodies followed by detection with HRP-Goat anti-Mouse IgG and iFluor® 430 tyramide™ (Left) or Alexa Fluor® 430 tyramide (Right). Fluorescence images were taken on a Keyence BZ-X710 fluorescence microscope equipped with a violet filter set.

Protocol

SDS

Catalog	Size	Price	Quantity
45096	200 Slides	Price

Overview

iFluor® 430 tyramide is optimized to a superior replacement for Opal 480 or other spectrally similar fluorescent tyramide conjugates or TSA reagents. Tyramide reagents can be used to detect extremely low-abundance targets in cells and tissues with significantly improved fluorescence signal than the direct fluorescence labeling reagents. In combination with our superior iFluor® dyes that have higher florescence intensity, increased photostability and enhanced water solubility, the iFluor® dye-labeled tyramide conjugates can generate fluorescence signal with significantly higher precision and sensitivity.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 430 Tyramide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	140.669 µL	703.344 µL	1.407 mL	7.033 mL	14.067 mL
5 mM	28.134 µL	140.669 µL	281.337 µL	1.407 mL	2.813 mL
10 mM	14.067 µL	70.334 µL	140.669 µL	703.344 µL	1.407 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	710.89 g/mol	=	mL	x	mM	=	mmol

Citations

View all 7 citations: Citation Explorer

CEBPB-high dormant tumor cells drive immune evasion via S100A8 orchestrated tumor-associated macrophages reprogramming

Authors:

Bai, Jin and Su, Huilan and Wang, Shunxi and Dong, Yang and Gao, Yong

Journal:

Theranostics (2026): 4883

The lipid-metabolic enzyme HSD17B12 drives lysosomal degradation of PD-L1 potentiating anti-tumor immunity in a mouse model

Authors:

Zhou, Zhihui and Lu, Ying and Li, Pan and Liu, Xin and Cheng, Wei and Chen, Hai-Ning and Dai, Lunzhi and Ren, Haiyan

Journal:

PLoS biology (2026): e3003603

CAR-T triggers TAM reeducation and adaptive anti-tumor response via TREM2 deficiency or CD40 agonist

Authors:

Liu, Ting and Gao, Huixin and Xi, Zhihui and Yu, TianTian and Gu, Yimei and Mai, Hanbing and Yuan, Hui and Liu, Yafang and Liu, Haikuan and Zhang, Qiaoxuan and others,

Journal:

Cell Reports Medicine (2026)

Immunostimulatory DNA Tetrahedron-Based Nanovaccine Combined With Immune Checkpoint PD-1 Blockade for Boosting Systemic Immune Responses Against Oral Squamous Cell Carcinoma

Authors:

Yang, Xueting and Wang, Yun and Yang, Yan and Zhang, Sicheng and Wang, Dianri and Luo, Yi and Shui, Chunyan and Cai, Yongcong and Yang, Ruoyi and Dong, Shuang and others,

Journal:

Aggregate (2025): e70061

Membrane progesterone receptor $\gamma$ (paqr5b) is essential for the formation of neurons in the zebrafish olfactory rosette

Authors:

Mustary, Umme Habiba and Maeno, Akiteru and Rahaman, Md Mostafizur and Ali, Md Hasan and Tokumoto, Toshinobu

Journal:

Scientific Reports (2024): 24354

References

View all 50 references: Citation Explorer

Phenoxy Radical Reactivity of Nucleic Acids: Practical Implications for Biotinylation.

Authors:

Wilbanks, Brandon and Garcia, Brian and Byrne, Shane and Dedon, Peter and Maher, L James

Journal:

Chembiochem : a European journal of chemical biology (2021): 1400-1404

Immunohistochemical Detection of 5-Hydroxymethylcytosine and 5-Carboxylcytosine in Sections of Zebrafish Embryos.

Authors:

Jessop, Peter and Gering, Martin

Journal:

Methods in molecular biology (Clifton, N.J.) (2021): 193-208

Accessibility-dependent topology studies of membrane proteins using a SpyTag/SpyCatcher protein-ligation system.

Authors:

Bae, Yoonji and Lee, Sang Kwon and Chae, Young Chan and Park, Chan Young and Kang, Sebyung

Journal:

International journal of biological macromolecules (2021): 171-178

Multiplexed In Situ Protein Profiling with High-Performance Cleavable Fluorescent Tyramide.

Authors:

Pham, Thai and Liao, Renjie and Labaer, Joshua and Guo, Jia

Journal:

Molecules (Basel, Switzerland) (2021)

Highly Sensitive and Multiplexed In Situ RNA Profiling with Cleavable Fluorescent Tyramide.

Authors:

Xiao, Lu and Labaer, Joshua and Guo, Jia

Journal:

Cells (2021)

Physical properties

Molecular weight	710.89
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.68
Correction factor (280 nm)	0.3
Extinction coefficient (cm ^-1 M ^-1)	20000 ¹
Excitation (nm)	433
Emission (nm)	497
Quantum yield	0.78 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Instrument settings

Fluorescence microscope
Excitation	Violet filter set
Emission	Violet filter set
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on April 18, 2026