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iFluor® 450 Styramide
Superior Replacement for Opal Polaris 480
Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. In combination with our superior iFluor® dyes that have higher florescence intensity, increased photostability and enhanced water solubility, the iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA utilizes the catalytic activity of horseradish peroxidase (HRP) for covalent deposition of fluorophores in situ. PSA radicals have much higher reactivity than tyramide radicals, making the PSA system much faster, more robust and sensitive than the traditional TSA reagents. Compared to tyramide reagents, the Styramide™ conjugates have ability to label the target at higher efficiency and thus generate significantly higher fluorescence signal. Styramide™ conjugates also allow significantly less consumption of primary antibody compared to standard directly conjugate method or tyramide amplification with the same level of sensitivity. iFluor® 450 Styramide is optimized to be alternative to Opal Polaris 480 or other spectrally similar fluorescent tyramide conjugates or TSA reagents.
Fluorescence IHC of formaldehyde-fixed paraffin-embedded tissue. Human lung adenocarcinoma sections were incubated with Mouse mAb EpCAM and then incubated with HRP-labeled Goat anti-mouse IgG secondary antibody followed by iFluor® 450 Styramide™ (Cat#45010) stain. Fluorescence images were taken using the FITC filter set.
Fluorescence IHC of formaldehyde-fixed paraffin-embedded tissue. Human lung adenocarcinoma sections were incubated with Mouse mAb EpCAM and then incubated with HRP-labeled Goat anti-mouse IgG secondary antibody followed by iFluor® 450 Styramide™ (Cat#45010) stain. Fluorescence images were taken using the FITC filter set.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
45010100 Slides
Price
 
Physical properties
Molecular weight683.78
SolventDMSO
Spectral properties
Correction factor (260 nm)0.45
Correction factor (280 nm)0.27
Extinction coefficient (cm -1 M -1)
40000
1
Excitation (nm)451
Emission (nm)502
Quantum yield
0.82
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Instrument settings
Fluorescence microscope
ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall/clear bottom
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Page updated on October 13, 2025