Name: iFluor® 460 Styramide
Brand: AAT Bioquest
SKU: 44902
Price: 329 USD
Availability: InStock

iFluor® 460 Styramide

Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. In combination with our superior iFluor® dyes that have higher florescence intensity, increased photostability and enhanced water solubility, the iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. Compared to tyramide reagents, the Styramide™ conjugates have ability to label the target at higher efficiency and thus generate significantly higher fluorescence signal. Styramide™ conjugates also allow significantly less consumption of primary antibody compared to standard directly conjugate method or tyramide amplification with the same level of sensitivity. iFluor® 460 Styramide is a new unique PSA™ reagent for multicolor application with our existing PSA™ and TSA reagents. It is optimized with the instruments equipped with the 460 nm laser or other equivalent light sources such as LEDs.

Fluorescence IHC of formaldehyde-fixed, paraffin-embedded human lung adenocarcinoma positive tissue using PSA<strong> ™</strong> amplified methods. Human lung adenocarcinoma positive tissue sections were stained with Mouse anti-EpCAM or Control Mouse IgG antibody and then incubated with polyHRP-labeled Goat anti-Mouse IgG secondary antibody followed by iFluor® 460 Styramide™ (Cat#44902).

Protocol

SDS

Catalog	Size	Price	Quantity
44902	100 Slides	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 460 Styramide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	102.536 µL	512.679 µL	1.025 mL	5.127 mL	10.254 mL
5 mM	20.507 µL	102.536 µL	205.071 µL	1.025 mL	2.051 mL
10 mM	10.254 µL	51.268 µL	102.536 µL	512.679 µL	1.025 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	975.27 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

Immunofluorescent Staining of Adult Murine Paraffin-Embedded Skeletal Tissue.

Authors:

Felsenthal, Neta and Zelzer, Elazar

Journal:

Methods in molecular biology (Clifton, N.J.) (2021): 337-344

An Advanced Method for the Immunohistochemical Detection of Nitric Oxide Synthase (NOS) in the Female Genital Tract.

Authors:

Ückert, Stefan and Richter, Karin and Fischer, Klaus-Dieter and Albrecht, Knut and Kuczyk, Markus A

Journal:

Analytical biochemistry (2021): 114264

Single-cell RNA sequencing of human liver reveals hepatic stellate cell heterogeneity.

Authors:

Payen, Valéry L and Lavergne, Arnaud and Alevra Sarika, Niki and Colonval, Megan and Karim, Latifa and Deckers, Manon and Najimi, Mustapha and Coppieters, Wouter and Charloteaux, Benoît and Sokal, Etienne M and El Taghdouini, Adil

Journal:

JHEP reports : innovation in hepatology (2021): 100278

Neutrophils use selective autophagy receptor Sqstm1/p62 to target Staphylococcus aureus for degradation in vivo in zebrafish.

Authors:

Gibson, Josie F and Prajsnar, Tomasz K and Hill, Christopher J and Tooke, Amy K and Serba, Justyna J and Tonge, Rebecca D and Foster, Simon J and Grierson, Andrew J and Ingham, Philip W and Renshaw, Stephen A and Johnston, Simon A

Journal:

Autophagy (2021): 1448-1457

Multiplex Immunohistochemistry Analysis of Melanoma Tumor-Infiltrating Lymphocytes.

Authors:

Nguyen, Thu and Kocovski, Nikolce and Macdonald, Sean and Yeang, Han Xian Aw and Wang, Minyu and Neeson, Paul J

Journal:

Methods in molecular biology (Clifton, N.J.) (2021): 557-572

Physical properties

Molecular weight	975.27
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.98
Correction factor (280 nm)	0.46
Extinction coefficient (cm ^-1 M ^-1)	80000 ¹
Excitation (nm)	468
Emission (nm)	493
Quantum yield	~0.8 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Instrument settings

Fluorescence microscope
Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom

Telephone
Fax
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Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on January 26, 2026