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iFluor® 460 Styramide
Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. In combination with our superior iFluor® dyes that have higher florescence intensity, increased photostability and enhanced water solubility, the iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. Compared to tyramide reagents, the Styramide™ conjugates have ability to label the target at higher efficiency and thus generate significantly higher fluorescence signal. Styramide™ conjugates also allow significantly less consumption of primary antibody compared to standard directly conjugate method or tyramide amplification with the same level of sensitivity. iFluor® 460 Styramide is a new unique PSA reagent for multicolor application with our existing PSA and TSA reagents. It is optimized with the instruments equipped with the 460 nm laser or other equivalent light sources such as LEDs.
Fluorescence IHC of formaldehyde-fixed, paraffin-embedded human lung adenocarcinoma positive tissue using PSA<strong>&nbsp;&trade;</strong>&nbsp;amplified methods. Human lung adenocarcinoma positive tissue sections were stained with&nbsp;Mouse anti-EpCAM or Control Mouse IgG antibody and then incubated with polyHRP-labeled Goat anti-Mouse IgG secondary antibody followed by iFluor® 460 Styramide&trade; (Cat#44902).
Fluorescence IHC of formaldehyde-fixed, paraffin-embedded human lung adenocarcinoma positive tissue using PSA<strong>&nbsp;&trade;</strong>&nbsp;amplified methods. Human lung adenocarcinoma positive tissue sections were stained with&nbsp;Mouse anti-EpCAM or Control Mouse IgG antibody and then incubated with polyHRP-labeled Goat anti-Mouse IgG secondary antibody followed by iFluor® 460 Styramide&trade; (Cat#44902).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
44902100 Slides
Price
 
Physical properties
Molecular weight975.27
SolventDMSO
Spectral properties
Correction factor (260 nm)0.98
Correction factor (280 nm)0.46
Extinction coefficient (cm -1 M -1)
80000
1
Excitation (nm)468
Emission (nm)493
Quantum yield
~0.8
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Instrument settings
Fluorescence microscope
ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall/clear bottom
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Page updated on October 13, 2025