iFluor®488-PEG12-dUTP *1 mM in TE Buffer (pH 7.5)*
The dye-modified deoxyuridine 5'-triphosphates are one of the most common methods to produce dye-labelled DNA via the conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This iFluor®488-PEG12-dUTP conjugate has the same spectral properties to ChromaTide™Alexa Fluor 488-dUTP. Its PEG12 spacer makes it much more effectively incorporated into DNAs by polymerase. It is used as a green fluorescence color with SpectrumGreen™ filter set. It provides a bright signal that has high photostability and is not affected by pH. ChromaTide™ and Alexa Fluor™ are the trademarks of ThermoFisher, and SpectrumGreen™ is the trademark of Vysis.
Spectrum
Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) | Correction Factor (656 nm) |
iFluor®555-PEG12-dUTP *1 mM in TE Buffer (pH 7.5)* | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 | - |
iFluor®647-PEG12-dUTP *1 mM in TE Buffer (pH 7.5)* | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 | 0.0793 |
References
View all 50 references: Citation Explorer
Mangiferin Inhibits Apoptosis in Doxorubicin-Induced Vascular Endothelial Cells via the Nrf2 Signaling Pathway.
Authors: Ismail, Mohammad Bani and Rajendran, Peramaiyan and AbuZahra, Hamad Mohammed and Veeraraghavan, Vishnu Priya
Journal: International journal of molecular sciences (2021)
Authors: Ismail, Mohammad Bani and Rajendran, Peramaiyan and AbuZahra, Hamad Mohammed and Veeraraghavan, Vishnu Priya
Journal: International journal of molecular sciences (2021)
Therapeutic effect of carbon monoxide-releasing molecule-3 on acute lung injury after hemorrhagic shock and resuscitation.
Authors: Kumada, Yuta and Takahashi, Toru and Shimizu, Hiroko and Nakamura, Ryu and Omori, Emiko and Inoue, Kazuyoshi and Morimatsu, Hiroshi
Journal: Experimental and therapeutic medicine (2019): 3429-3440
Authors: Kumada, Yuta and Takahashi, Toru and Shimizu, Hiroko and Nakamura, Ryu and Omori, Emiko and Inoue, Kazuyoshi and Morimatsu, Hiroshi
Journal: Experimental and therapeutic medicine (2019): 3429-3440
Activation of β-catenin signaling in aggrecan-expressing cells in temporomandibular joint causes osteoarthritis-like defects.
Authors: Hui, Tianqian and Zhou, Yachuan and Wang, Tingyu and Li, Jun and Zhang, Shanxing and Liao, Lifan and Gu, Jianhong and Ye, Ling and Zhao, Lan and Chen, Di
Journal: International journal of oral science (2018): 13
Authors: Hui, Tianqian and Zhou, Yachuan and Wang, Tingyu and Li, Jun and Zhang, Shanxing and Liao, Lifan and Gu, Jianhong and Ye, Ling and Zhao, Lan and Chen, Di
Journal: International journal of oral science (2018): 13
Adenovirus-delivered wwox inhibited lung cancer growth in vivo in a mouse model.
Authors: Zhou, Y and Shou, F and Zhang, H and You, Q
Journal: Cancer gene therapy (2016): 1-6
Authors: Zhou, Y and Shou, F and Zhang, H and You, Q
Journal: Cancer gene therapy (2016): 1-6
Corn silk maysin induces apoptotic cell death in PC-3 prostate cancer cells via mitochondria-dependent pathway.
Authors: Lee, Jisun and Lee, Seul and Kim, Sun-Lim and Choi, Ji Won and Seo, Jeong Yeon and Choi, Doo Jin and Park, Yong Il
Journal: Life sciences (2014): 47-55
Authors: Lee, Jisun and Lee, Seul and Kim, Sun-Lim and Choi, Ji Won and Seo, Jeong Yeon and Choi, Doo Jin and Park, Yong Il
Journal: Life sciences (2014): 47-55