iFluor®488-PEG12-dUTP *1 mM in TE Buffer (pH 7.5)*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 2274.60 |
Solvent | Water |
Spectral properties
Correction Factor (260 nm) | 0.21 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 750001 |
Excitation (nm) | 491 |
Emission (nm) | 516 |
Quantum yield | 0.91 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
Molecular weight 2274.60 | Correction Factor (260 nm) 0.21 | Correction Factor (280 nm) 0.11 | Extinction coefficient (cm -1 M -1) 750001 | Excitation (nm) 491 | Emission (nm) 516 | Quantum yield 0.91 |
The dye-modified deoxyuridine 5'-triphosphates are one of the most common methods to produce dye-labelled DNA via the conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This iFluor®488-PEG12-dUTP conjugate has the same spectral properties to ChromaTide™Alexa Fluor 488-dUTP. Its PEG12 spacer makes it much more effectively incorporated into DNAs by polymerase. It is used as a green fluorescence color with SpectrumGreen™ filter set. It provides a bright signal that has high photostability and is not affected by pH. ChromaTide™ and Alexa Fluor™ are the trademarks of ThermoFisher, and SpectrumGreen™ is the trademark of Vysis.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of iFluor®488-PEG12-dUTP *1 mM in TE Buffer (pH 7.5)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 43.964 µL | 219.819 µL | 439.638 µL | 2.198 mL | 4.396 mL |
5 mM | 8.793 µL | 43.964 µL | 87.928 µL | 439.638 µL | 879.275 µL |
10 mM | 4.396 µL | 21.982 µL | 43.964 µL | 219.819 µL | 439.638 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Correction Factor (260 nm) | 0.21 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 750001 |
Excitation (nm) | 491 |
Emission (nm) | 516 |
Quantum yield | 0.91 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) | Correction Factor (656 nm) |
iFluor®555-PEG12-dUTP *1 mM in TE Buffer (pH 7.5)* | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 | - |
iFluor®647-PEG12-dUTP *1 mM in TE Buffer (pH 7.5)* | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 | 0.0793 |
References
View all 50 references: Citation Explorer
Mangiferin Inhibits Apoptosis in Doxorubicin-Induced Vascular Endothelial Cells via the Nrf2 Signaling Pathway.
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Journal: International journal of molecular sciences (2021)
Authors: Ismail, Mohammad Bani and Rajendran, Peramaiyan and AbuZahra, Hamad Mohammed and Veeraraghavan, Vishnu Priya
Journal: International journal of molecular sciences (2021)
Therapeutic effect of carbon monoxide-releasing molecule-3 on acute lung injury after hemorrhagic shock and resuscitation.
Authors: Kumada, Yuta and Takahashi, Toru and Shimizu, Hiroko and Nakamura, Ryu and Omori, Emiko and Inoue, Kazuyoshi and Morimatsu, Hiroshi
Journal: Experimental and therapeutic medicine (2019): 3429-3440
Authors: Kumada, Yuta and Takahashi, Toru and Shimizu, Hiroko and Nakamura, Ryu and Omori, Emiko and Inoue, Kazuyoshi and Morimatsu, Hiroshi
Journal: Experimental and therapeutic medicine (2019): 3429-3440
Activation of β-catenin signaling in aggrecan-expressing cells in temporomandibular joint causes osteoarthritis-like defects.
Authors: Hui, Tianqian and Zhou, Yachuan and Wang, Tingyu and Li, Jun and Zhang, Shanxing and Liao, Lifan and Gu, Jianhong and Ye, Ling and Zhao, Lan and Chen, Di
Journal: International journal of oral science (2018): 13
Authors: Hui, Tianqian and Zhou, Yachuan and Wang, Tingyu and Li, Jun and Zhang, Shanxing and Liao, Lifan and Gu, Jianhong and Ye, Ling and Zhao, Lan and Chen, Di
Journal: International journal of oral science (2018): 13
Adenovirus-delivered wwox inhibited lung cancer growth in vivo in a mouse model.
Authors: Zhou, Y and Shou, F and Zhang, H and You, Q
Journal: Cancer gene therapy (2016): 1-6
Authors: Zhou, Y and Shou, F and Zhang, H and You, Q
Journal: Cancer gene therapy (2016): 1-6
Corn silk maysin induces apoptotic cell death in PC-3 prostate cancer cells via mitochondria-dependent pathway.
Authors: Lee, Jisun and Lee, Seul and Kim, Sun-Lim and Choi, Ji Won and Seo, Jeong Yeon and Choi, Doo Jin and Park, Yong Il
Journal: Life sciences (2014): 47-55
Authors: Lee, Jisun and Lee, Seul and Kim, Sun-Lim and Choi, Ji Won and Seo, Jeong Yeon and Choi, Doo Jin and Park, Yong Il
Journal: Life sciences (2014): 47-55
Hepatoprotective properties of sesamin against CCl4 induced oxidative stress-mediated apoptosis in mice via JNK pathway.
Authors: Ma, Jie-Qiong and Ding, Jie and Zhang, Li and Liu, Chan-Min
Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (2014): 41-8
Authors: Ma, Jie-Qiong and Ding, Jie and Zhang, Li and Liu, Chan-Min
Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (2014): 41-8
Inhibition of APE1/Ref-1 redox activity rescues human retinal pigment epithelial cells from oxidative stress and reduces choroidal neovascularization.
Authors: Li, Y and Liu, X and Zhou, T and Kelley, M R and Edwards, P and Gao, H and Qiao, X
Journal: Redox biology (2014): 485-94
Authors: Li, Y and Liu, X and Zhou, T and Kelley, M R and Edwards, P and Gao, H and Qiao, X
Journal: Redox biology (2014): 485-94
Immunoliposome co-delivery of bufalin and anti-CD40 antibody adjuvant induces synergetic therapeutic efficacy against melanoma.
Authors: Li, Ying and Yuan, Jiani and Yang, Qian and Cao, Wei and Zhou, Xuanxuan and Xie, Yanhua and Tu, Honghai and Zhang, Ya and Wang, Siwang
Journal: International journal of nanomedicine (2014): 5683-700
Authors: Li, Ying and Yuan, Jiani and Yang, Qian and Cao, Wei and Zhou, Xuanxuan and Xie, Yanhua and Tu, Honghai and Zhang, Ya and Wang, Siwang
Journal: International journal of nanomedicine (2014): 5683-700
Differential expression of the P2X7 receptor in ovarian surface epithelium during the oestrous cycle in the mouse.
Authors: Vázquez-Cuevas, F G and Cruz-Rico, A and Garay, E and García-Carrancá, A and Pérez-Montiel, D and Juárez, B and Arellano, R O
Journal: Reproduction, fertility, and development (2013): 971-84
Authors: Vázquez-Cuevas, F G and Cruz-Rico, A and Garay, E and García-Carrancá, A and Pérez-Montiel, D and Juárez, B and Arellano, R O
Journal: Reproduction, fertility, and development (2013): 971-84
Effects of ionizing radiation on Capsicum baccatum var. pendulum (Solanaceae).
Authors: Scaldaferro, M A and Prina, A R and Moscone, E A and Kwasniewska, J
Journal: Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine (2013): 103-8
Authors: Scaldaferro, M A and Prina, A R and Moscone, E A and Kwasniewska, J
Journal: Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine (2013): 103-8
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