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iFluor® 488-streptavidin conjugate
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. A variety of the complementary biotinylated reagents are available from numerous commercial vendors.This iFluor® 488-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with iFluor® 488 covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. iFluor® 488-streptavidin conjugates has fluorescence excitation and emission maxima of ~491 nm and ~514 nm respectively. These spectral characteristics make it an excellent alternative to Alexa Fluor® 488-streptavidin conjugate (Alexa Fluor® is the trademark of Invitrogen). It is a very valuable tool for biotin-streptavidin-based biological assays and tests.
HeLa cells were incubated with mouse anti-tubulin and biotin goat anti-mouse IgG followed by AAT's iFluorTM 488-streptavidin conjugate (Green, Left) or streptavidin conjugated with Alexa Fluor® 488 (Green, Right), respectively. Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17530).
HeLa cells were incubated with mouse anti-tubulin and biotin goat anti-mouse IgG followed by AAT's iFluorTM 488-streptavidin conjugate (Green, Left) or streptavidin conjugated with Alexa Fluor® 488 (Green, Right), respectively. Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17530).
protocol
Protocol
COA
CatalogSize
Price
Quantity
16955200 ug
Price
169851 mg
Price
 
Physical properties
SolventWater
Spectral properties
Correction factor (260 nm)0.21
Correction factor (280 nm)0.11
Extinction coefficient (cm -1 M -1)
75000
1
Excitation (nm)491
Emission (nm)516
Quantum yield
0.9
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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Page updated on October 8, 2025