Name: iFluor® 488-streptavidin conjugate
Brand: AAT Bioquest
SKU: 16955
Availability: InStock

iFluor® 488-streptavidin conjugate

iFluor® 488-Streptavidin Conjugate is a green-fluorescent, biotin-binding probe for sensitive detection of biotinylated proteins, antibodies, nucleic acids, and other biomolecules in fluorescence-based assays.

High-Affinity Biotin Binding: Streptavidin enables specific detection of biotinylated targets.
Spectral Properties: Excitation at 491 nm and emission at 516 nm.
Fluorescence Performance: Bright, photostable fluorescence with a quantum yield of 0.90.
Spectral Compatibility: Alternative to Alexa Fluor® 488-streptavidin.
Applications: Suitable for indirect immunofluorescence staining and biotin-streptavidin-based assays.

HeLa cells were incubated with mouse anti-tubulin and biotin goat anti-mouse IgG followed by AAT's iFluorTM 488-streptavidin conjugate (Green, Left) or streptavidin conjugated with Alexa Fluor® 488 (Green, Right), respectively. Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17530).

Datasheet

Catalog	Size	Price	Quantity
16955	200 ug	Price
16985	1 mg	Price

Overview

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. A variety of the complementary biotinylated reagents are available from numerous commercial vendors.This iFluor® 488-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with iFluor® 488 covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. iFluor® 488-streptavidin conjugates has fluorescence excitation and emission maxima of ~491 nm and ~514 nm respectively. These spectral characteristics make it an excellent alternative to Alexa Fluor® 488-streptavidin conjugate (Alexa Fluor® is the trademark of Invitrogen). It is a very valuable tool for biotin-streptavidin-based biological assays and tests.

Citations

View all 7 citations: Citation Explorer

CAR-T triggers TAM reeducation and adaptive anti-tumor response via TREM2 deficiency or CD40 agonist

Authors:

Liu, Ting and Gao, Huixin and Xi, Zhihui and Yu, TianTian and Gu, Yimei and Mai, Hanbing and Yuan, Hui and Liu, Yafang and Liu, Haikuan and Zhang, Qiaoxuan and others,

Journal:

Cell Reports Medicine (2026)

Glycan Signatures on Neutrophils in an Equine Model for Autoimmune Uveitis

Authors:

Sprenzel, Carolin J and Amann, Barbara and Deeg, Cornelia A and Degroote, Roxane L

Journal:

Biomolecules (2025): 1444

Isolation, characterization, and structure-based engineering of a neutralizing nanobody against SARS-CoV-2

Authors:

Li, Tingting and Zhou, Bingjie and Li, Yaning and Huang, Suqiong and Luo, Zhipu and Zhou, Yuanze and Lai, Yanling and Gautam, Anupriya and Bourgeau, Salome and Wang, Shurui and others,

Journal:

International Journal of Biological Macromolecules (2022): 1379--1388

A synthetic nanobody targeting RBD protects hamsters from SARS-CoV-2 infection

Authors:

Li, Tingting and Cai, Hongmin and Yao, Hebang and Zhou, Bingjie and Zhang, Ning and van Vlissingen, Martje Fentener and Kuiken, Thijs and Han, Wenyu and GeurtsvanKessel, Corine H and Gong, Yuhuan and others,

Journal:

Nature communications (2021): 1--13

A potent synthetic nanobody targets RBD and protects mice from SARS-CoV-2 infection

Authors:

Li, Tingting and Cai, Hongmin and Yao, Hebang and Zhou, Bingjie and Zhang, Ning and Gong, Yuhuan and Zhao, Yapei and Shen, Quan and Qin, Wenming and Hutter, Cedric AJ and others,

Journal:

BioRxiv (2020)

References

View all 47 references: Citation Explorer

A streptavidin paramagnetic-particle based competition assay for the evaluation of the optical selectivity of quadruplex nucleic acid fluorescent probes

Authors:

Largy E, Hamon F, Teulade-Fichou MP.

Journal:

Methods. (2012)

Biotin-4-fluorescein based fluorescence quenching assay for determination of biotin binding capacity of streptavidin conjugated quantum dots

Authors:

Mittal R, Bruchez MP.

Journal:

Bioconjug Chem (2011): 362

Iminobiotin binding induces large fluorescent enhancements in avidin and streptavidin fluorescent conjugates and exhibits diverging pH-dependent binding affinities

Authors:

Raphael MP, Rappole CA, Kurihara LK, Christodoulides JA, Qadri SN, Byers JM.

Journal:

J Fluoresc (2011): 647

Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex

Authors:

Kim JH, Chang TM, Graham AN, Choo KH, Kalitsis P, Hudson DF.

Journal:

BMC Biochem (2010): 50

Determination of 17beta-oestradiol by fluorescence immunoassay with streptavidin-conjugated quantum dots as label

Authors:

Sun M, Du L, Gao S, Bao Y, Wang S.

Journal:

Steroids (2010): 400

Physical properties

Solvent

Water

Spectral properties

Correction factor (260 nm)	0.21
Correction factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 23, 2026