Name: iFluor® 514-streptavidin conjugate
Brand: AAT Bioquest
SKU: 16956
Availability: InStock

iFluor® 514-streptavidin conjugate

iFluor® 514-Streptavidin Conjugate is a green-fluorescent, biotin-binding probe for sensitive detection of biotinylated proteins, antibodies, nucleic acids, and other biomolecules in fluorescence-based assays.

High-Affinity Biotin Binding: Streptavidin enables specific detection of biotinylated targets.
Spectral Properties: Excitation at 511 nm and emission at 527 nm.
Fluorescence Performance: Bright and photostable fluorescence.
Spectral Compatibility: Provides an alternative to Alexa Fluor® 514.
Applications: Suitable for indirect immunofluorescence staining and biotin-streptavidin-based assays.

Fluorescence image of HeLa cells stained for microtubules. Fixed HeLa cells were immunostained for tubulin using a biotinylated goat anti-mouse IgG secondary antibody, followed by Streptavidin-iFluor® 514 (Cat. #16956) for microtubule detection. Cells were counterstained with Hoechst 33342 (Cat. #17530) for nuclear staining. Images were acquired using FITC and DAPI filter sets and overlaid to show microtubules in green and nuclei in blue.

Datasheet

Catalog	Size	Price	Quantity
16956	200 ug	Price
16986	1 mg	Price

Overview

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules since streptavidin has a very high binding affinity for biotin. A variety of complementary biotinylated reagents are available from numerous commercial vendors. This iFluor® 514-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with iFluor® 514 covalently attached (as the fluorescent label). It is commonly used as a second-step reagent for indirect immunofluorescent staining in conjunction with biotinylated primary antibodies. iFluor® 514-streptavidin conjugates has fluorescence excitation and emission maxima of ~510 nm and ~527 nm respectively. These spectral characteristics make it an excellent alternative to Alexa Fluor® 514-streptavidin conjugate (Alexa Fluor® is the trademark of Invitrogen). It is a very valuable tool for biotin-streptavidin-based biological assays and tests.

References

View all 47 references: Citation Explorer

A streptavidin paramagnetic-particle based competition assay for the evaluation of the optical selectivity of quadruplex nucleic acid fluorescent probes

Authors:

Largy E, Hamon F, Teulade-Fichou MP.

Journal:

Methods. (2012)

Biotin-4-fluorescein based fluorescence quenching assay for determination of biotin binding capacity of streptavidin conjugated quantum dots

Authors:

Mittal R, Bruchez MP.

Journal:

Bioconjug Chem (2011): 362

Iminobiotin binding induces large fluorescent enhancements in avidin and streptavidin fluorescent conjugates and exhibits diverging pH-dependent binding affinities

Authors:

Raphael MP, Rappole CA, Kurihara LK, Christodoulides JA, Qadri SN, Byers JM.

Journal:

J Fluoresc (2011): 647

Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex

Authors:

Kim JH, Chang TM, Graham AN, Choo KH, Kalitsis P, Hudson DF.

Journal:

BMC Biochem (2010): 50

Determination of 17beta-oestradiol by fluorescence immunoassay with streptavidin-conjugated quantum dots as label

Authors:

Sun M, Du L, Gao S, Bao Y, Wang S.

Journal:

Steroids (2010): 400

Physical properties

Molecular weight	~52000
Solvent	Water

Spectral properties

Absorbance (nm)	511
Correction factor (260 nm)	0.265
Correction factor (280 nm)	0.116
Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	511
Emission (nm)	527
Quantum yield	0.83 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 28, 2026