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iFluor® 514 Styramide
Superior Replacement for Opal 540
Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. In combination with our superior iFluor® dyes that have higher fluorescence intensity, increased photostability, and enhanced water solubility, the iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA utilizes the catalytic activity of horseradish peroxidase (HRP) for the covalent deposition of fluorophores in situ. PSA radicals have much higher reactivity than tyramide radicals, making the PSA system much faster, more robust, and more sensitive than traditional TSA reagents. Compared to tyramide reagents, the Styramide™ conjugates can label the target at higher efficiency and thus generate a significantly higher fluorescence signal. Styramide™ conjugates also allow substantially less consumption of primary antibodies compared to the standard directly conjugate method or tyramide amplification with the same level of sensitivity. iFluor® 514 Styramide is optimized to be a superior replacement for Opal Polaris 540 or other spectrally similar fluorescent tyramide conjugates or TSA reagents.
Fluorescence IHC of formaldehyde-fixed, paraffin-embedded human lung adenocarcinoma positive tissue using PSA™ amplified methods. Human lung adenocarcinoma positive tissue sections were stained with Mouse anti-EpCAM or Control Mouse IgG antibody and then incubated with polyHRP-labeled Goat anti-Mouse IgG secondary antibody followed by iFluor® 514 Styramide™ (Cat. 45022).
Fluorescence IHC of formaldehyde-fixed, paraffin-embedded human lung adenocarcinoma positive tissue using PSA™ amplified methods. Human lung adenocarcinoma positive tissue sections were stained with Mouse anti-EpCAM or Control Mouse IgG antibody and then incubated with polyHRP-labeled Goat anti-Mouse IgG secondary antibody followed by iFluor® 514 Styramide™ (Cat. 45022).
protocol
Protocol
sds
SDS
CatalogSize
Price
Quantity
45022100 Slides
Price
 
Physical properties
Molecular weight1195.24
SolventDMSO
Spectral properties
Absorbance (nm)511
Correction factor (260 nm)0.265
Correction factor (280 nm)0.116
Extinction coefficient (cm -1 M -1)
75000
1
Excitation (nm)511
Emission (nm)527
Quantum yield
0.83
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Instrument settings
Fluorescence microscope
ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall/clear bottom
Documents
Protocol
Safety Data Sheet
Certificate of Origin
Certificate of Analysis
Brochure: Power Styramide™ Signal Amplification (PSA™)
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Page updated on October 30, 2025