iFluor® 514 Styramide *Superior Replacement for Opal 540*

iFluor® 514 Styramide

Superior Replacement for Opal 540

Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. In combination with our superior iFluor® dyes that have higher fluorescence intensity, increased photostability, and enhanced water solubility, the iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA utilizes the catalytic activity of horseradish peroxidase (HRP) for the covalent deposition of fluorophores in situ. PSA radicals have much higher reactivity than tyramide radicals, making the PSA system much faster, more robust, and more sensitive than traditional TSA reagents. Compared to tyramide reagents, the Styramide™ conjugates can label the target at higher efficiency and thus generate a significantly higher fluorescence signal. Styramide™ conjugates also allow substantially less consumption of primary antibodies compared to the standard directly conjugate method or tyramide amplification with the same level of sensitivity. iFluor® 514 Styramide is optimized to be a superior replacement for Opal Polaris 540 or other spectrally similar fluorescent tyramide conjugates or TSA reagents.

Fluorescence IHC of formaldehyde-fixed, paraffin-embedded human lung adenocarcinoma positive tissue using PSA™ amplified methods. Human lung adenocarcinoma positive tissue sections were stained with Mouse anti-EpCAM or Control Mouse IgG antibody and then incubated with polyHRP-labeled Goat anti-Mouse IgG secondary antibody followed by iFluor® 514 Styramide™ (Cat. 45022).

Catalog	Size	Price	Quantity
45022	100 Slides	Price

References

View all 50 references: Citation Explorer

A novel chemiluminescence imaging immunosensor for prostate specific antigen detection based on a multiple signal amplification strategy.

Authors:

Zhao, Li-Zhen and Fu, Yi-Zhuo and Ren, Shu-Wei and Cao, Jun-Tao and Liu, Yan-Ming

Journal:

Biosensors & bioelectronics (2021): 112729

Imaging sensor array coupled with dual-signal amplification strategy for ultrasensitive chemiluminescence immunoassay of multiple mycotoxins.

Authors:

Zong, Chen and Jiang, Fan and Wang, Xiaoyu and Li, Ping and Xu, Linru and Yang, Hua

Journal:

Biosensors & bioelectronics (2021): 112998

Tyramide Signal-Amplified Immunofluorescence of MYCN and MYC in Human Tissue Specimens and Cell Line Cultures.

Authors:

Schafer, Johanna M and Pietenpol, Jennifer A

Journal:

Bio-protocol (2020): e3677

Cascade signal amplification for sensitive detection of exosomes by integrating tyramide and surface-initiated enzymatic polymerization.

Authors:

Huang, Zhipeng and Lin, Qiuyuan and Yang, Bin and Ye, Xin and Chen, Hui and Weng, Wenhao and Kong, Jilie

Journal:

Chemical communications (Cambridge, England) (2020): 12793-12796

Detection of Cytokine Receptors Using Tyramide Signal Amplification for Immunofluorescence.

Authors:

Wang, Herui and Pangilinan, Ryan L and Zhu, Yan

Journal:

Methods in molecular biology (Clifton, N.J.) (2020): 89-97

Physical properties

Molecular weight	1195.24
Solvent	DMSO

Spectral properties

Absorbance (nm)	511
Correction factor (260 nm)	0.265
Correction factor (280 nm)	0.116
Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	511
Emission (nm)	527
Quantum yield	0.83 ¹

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Instrument settings

Fluorescence microscope
Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom

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Page updated on October 13, 2025