Name: iFluor® 532-dUTP *1 mM in TE Buffer (pH 7.5)*
Brand: AAT Bioquest
SKU: 17046
Price: 118 USD
Availability: InStock

iFluor® 532-dUTP

1 mM in TE Buffer (pH 7.5)

iFluor® 532-dUTP is a yellow-fluorescent nucleotide analog for enzymatic incorporation into DNA, enabling the preparation of labeled probes for nucleic acid detection and hybridization applications.

Enzymatic DNA Labeling: Enables fluorescent nucleotide incorporation during DNA synthesis.
Spectral Properties: Excitation at 537 nm and emission at 560 nm.
Fluorescence Performance: Bright, photostable fluorescence compatible with SpectrumGold™ filter sets.
Applications: Supports PCR, nick translation, random priming, and reverse transcription.

Datasheet

SDS

Catalog	Size	Price	Quantity
17046	25 nmoles	Price

Overview

Dye-modified deoxyuridine 5'-triphosphates are widely used for the production of dye-labeled DNA via conventional enzymatic incorporation techniques, including reverse transcription, nick translation, random primed labeling, and PCR. These enzymatic fluorescence labeling methods are commonly employed in both fluorescence in situ hybridization (FISH) probes and microarray-based experiments. The iFluor® 532-dUTP conjugate, which emits yellow fluorescence, is compatible with the SpectrumGold™ filter set (SpectrumGold™ is a trademark of Vysis). Compared to Alexa Fluor® 532-labeled dNTPs, iFluor® 532 offers a significantly brighter signal, enhanced photostability, and greater resilience to pH fluctuations, maintaining stability even in higher pH conditions (e.g., pH 8-10, commonly used in DNA sequencing).

References

View all 49 references: Citation Explorer

Fluorescence in situ hybridization-negative intra-articular myxoid liposarcoma with complex rearrangements involving EWSR1::DDIT3 detected using nanopore sequencing.

Authors:

Makise, Naohiro and Lin, Jason and Kageyama, Hajime and Takeda, Naoki and Oikawa, Mariko and Sugiyama, Takahiro and Kawana, Hidetada and Araki, Akinobu and Kinoshita, Hideyuki and Kamoda, Hiroto and Hagiwara, Yoko and Yoshida, Akihiko and Yonemoto, Tsukasa and Kawazu, Masahito and Itami, Makiko

Journal:

Pathology international (2024)

A Method Based on a Modified Fluorescence In Situ Hybridization (FISH) Approach for the Sensing of Staphylococcus aureus from Nasal Samples.

Authors:

Sami, Amtul Jamil and Bilal, Sehrish and Alam, Sadaf and Khalid, Madeeha and Mangat, Hammad Ahmad

Journal:

Applied biochemistry and biotechnology (2024)

In-situ evaluation the fluctuation of hypochlorous acid in acute liver injury mice models with a mitochondria-targeted NIR ratiometric fluorescent probe.

Authors:

Liang, Yueyin and Zhang, Chunjie and Meng, Zhiyuan and Gong, Shuai and Tian, Jixiang and Li, Ruoming and Wang, Zhonglong and Wang, Shifa

Journal:

Talanta (2024): 126355

A New Peptide Nucleic Acid Fluorescence In Situ Hybridization Probe for the Specific Detection of Salmonella Species in Food Matrices.

Authors:

Sousa, Mário and Rocha, Rui and Araújo, Daniela and Castro, Joana and Barbosa, Ana and Azevedo, Nuno F and Cerqueira, Laura and Almeida, Carina

Journal:

Foodborne pathogens and disease (2024): 298-305

Formamide denaturation of double-stranded DNA for fluorescence in situ hybridization (FISH) distorts nanoscale chromatin structure.

Authors:

Shim, Anne R and Frederick, Jane and Pujadas, Emily M and Kuo, Tiffany and Ye, I Chae and Pritchard, Joshua A and Dunton, Cody L and Gonzalez, Paola Carrillo and Acosta, Nicolas and Jain, Surbhi and Anthony, Nicholas M and Almassalha, Luay M and Szleifer, Igal and Backman, Vadim

Journal:

PloS one (2024): e0301000

Physical properties

Solvent

Water

Spectral properties

Absorbance (nm)	536
Correction factor (260 nm)	0.26
Correction factor (280 nm)	0.16
Extinction coefficient (cm ^-1 M ^-1)	90000 ¹
Excitation (nm)	537
Emission (nm)	560
Quantum yield	0.68 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 26, 2026