Name: iFluor® 532-dUTP *1 mM in TE Buffer (pH 7.5)*
Brand: AAT Bioquest
SKU: 17046
Price: 118 USD
Availability: InStock

iFluor® 532-dUTP

1 mM in TE Buffer (pH 7.5)

Dye-modified deoxyuridine 5'-triphosphates are widely used for the production of dye-labeled DNA via conventional enzymatic incorporation techniques, including reverse transcription, nick translation, random primed labeling, and PCR. These enzymatic fluorescence labeling methods are commonly employed in both fluorescence in situ hybridization (FISH) probes and microarray-based experiments. The iFluor® 532-dUTP conjugate, which emits yellow fluorescence, is compatible with the SpectrumGold™ filter set (SpectrumGold™ is a trademark of Vysis). Compared to Alexa Fluor® 532-labeled dNTPs, iFluor® 532 offers a significantly brighter signal, enhanced photostability, and greater resilience to pH fluctuations, maintaining stability even in higher pH conditions (e.g., pH 8-10, commonly used in DNA sequencing).

Datasheet

SDS

Catalog	Size	Price	Quantity
17046	25 nmoles	Price

References

View all 50 references: Citation Explorer

Fluorescence in situ hybridization-negative intra-articular myxoid liposarcoma with complex rearrangements involving EWSR1::DDIT3 detected using nanopore sequencing.

Authors:

Makise, Naohiro and Lin, Jason and Kageyama, Hajime and Takeda, Naoki and Oikawa, Mariko and Sugiyama, Takahiro and Kawana, Hidetada and Araki, Akinobu and Kinoshita, Hideyuki and Kamoda, Hiroto and Hagiwara, Yoko and Yoshida, Akihiko and Yonemoto, Tsukasa and Kawazu, Masahito and Itami, Makiko

Journal:

Pathology international (2024)

In-situ evaluation the fluctuation of hypochlorous acid in acute liver injury mice models with a mitochondria-targeted NIR ratiometric fluorescent probe.

Authors:

Liang, Yueyin and Zhang, Chunjie and Meng, Zhiyuan and Gong, Shuai and Tian, Jixiang and Li, Ruoming and Wang, Zhonglong and Wang, Shifa

Journal:

Talanta (2024): 126355

A New Peptide Nucleic Acid Fluorescence In Situ Hybridization Probe for the Specific Detection of Salmonella Species in Food Matrices.

Authors:

Sousa, Mário and Rocha, Rui and Araújo, Daniela and Castro, Joana and Barbosa, Ana and Azevedo, Nuno F and Cerqueira, Laura and Almeida, Carina

Journal:

Foodborne pathogens and disease (2024): 298-305

Formamide denaturation of double-stranded DNA for fluorescence in situ hybridization (FISH) distorts nanoscale chromatin structure.

Authors:

Shim, Anne R and Frederick, Jane and Pujadas, Emily M and Kuo, Tiffany and Ye, I Chae and Pritchard, Joshua A and Dunton, Cody L and Gonzalez, Paola Carrillo and Acosta, Nicolas and Jain, Surbhi and Anthony, Nicholas M and Almassalha, Luay M and Szleifer, Igal and Backman, Vadim

Journal:

PloS one (2024): e0301000

The Application of Fluorescence In Situ Hybridization in the Prescreening of Veronica Hybrids.

Authors:

Park, Hye-Wan and Sevilleno, Samantha Serafin and Ha, My Khanh Tran Thi and Cabahug-Braza, Raisa Aone and Yi, Ji-Hun and Lim, Ki-Byung and Cho, Wonwoo and Hwang, Yoon-Jung

Journal:

Plants (Basel, Switzerland) (2024)

Physical properties

Solvent

Water

Spectral properties

Absorbance (nm)	536
Correction factor (260 nm)	0.26
Correction factor (280 nm)	0.16
Extinction coefficient (cm ^-1 M ^-1)	90000 ¹
Excitation (nm)	537
Emission (nm)	560
Quantum yield	0.68 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on March 15, 2026