Wheat Germ Agglutinin (WGA)

iFluor® 532-Wheat Germ Agglutinin (WGA) Conjugate

iFluor® 532-Wheat Germ Agglutinin (WGA) Conjugate is a yellow-fluorescent lectin probe for detecting cell-surface glycoconjugates and visualizing cellular membranes in live and fixed cells.

Selective Glycoconjugate Binding: Recognizes N-acetylglucosamine and sialic acid residues.
Spectral Properties: Excitation at 537 nm and emission at 560 nm.
Cell Membrane Labeling: Membrane-impermeant probe enables fluorescent staining of cell-surface glycoconjugates.
Filter Compatibility: Supports fluorescence imaging using standard Cy3/TRITC filter sets.
Applications: Suitable for live-cell staining, fixed-cell imaging, and intracellular glycoconjugate localization following permeabilization.

Live and fixed HeLa cells were stained with iFluor® 532-Wheat Germ Agglutinin (WGA) Conjugate at 10 µg/mL for 30 minutes. The image was acquired on a fluorescence microscope using Cy3/TRITC filter set.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
25532	1 mg	Price

Overview

Wheat germ agglutinin (WGA) is a well-studied lectin known for its binding affinity to N-acetyl-D-glucosamine and sialic acid, making it a valuable tool in various biological applications. Its interaction with glycoconjugates enables widespread use of WGA derivatives and conjugates for fluorescence imaging and analysis, facilitating the labeling of yeast bud scars, fibrotic scar tissue, and the cell membranes of gram bacteria and mammalian cells. WGA specifically targets sequences of β-1,4-GlcNAc-linked residues known as chitodextrins. Each monomer contains two identical, non-interacting binding sites complementary to 3 or 4 β-1,4-GlcNAc units. Among the monosaccharides tested, only GlcNAc shows strong binding to WGA, while ManNAc demonstrates no binding, and GalNAc exhibits weak binding. The iFluor® 532 conjugate of WGA possesses a bright, red fluorescence characteristic of the iFluor® 532 dye (Ex/Em = 537/560 nm). Notably, the iFluor® 532 WGA conjugate, like Alexa Fluor® 532 WGA conjugate, retains its ability to bind to sialic acid and N-acetylglucosaminyl residues, enhancing its utility in fluorescence imaging and analysis for various scientific investigations.

References

View all 10 references: Citation Explorer

Chitin distribution in the Oithona digestive and reproductive systems revealed by fluorescence microscopy.

Authors:

Sugier, Kevin and Vacherie, Benoit and Cornils, Astrid and Wincker, Patrick and Jamet, Jean-Louis and Madoui, Mohammed-Amin

Journal:

PeerJ (2018): e4685

Chairside molecular imaging of aberrant glycosylation in subjects with suspicious oral lesions using fluorescently labeled wheat germ agglutinin.

Authors:

Baeten, John and Johnson, Alexander and Sunny, Sumsum and Suresh, Amritha and Birur, Praveen and Uma, K and Kademani, Deepak

Journal:

Head & neck (2018): 292-301

Antifungal curcumin promotes chitin accumulation associated with decreased virulence of Sporothrix schenckii.

Authors:

Huang, Lilin and Zhang, Jing and Song, Tianzhang and Yuan, Liyan and Zhou, Junjie and Yin, Hongling and He, Tailong and Gao, Wenchao and Sun, Yao and Hu, Xuchu and Huang, Huaiqiu

Journal:

International immunopharmacology (2016): 263-270

Endothelial glycocalyx structure in the intact carotid artery: a two-photon laser scanning microscopy study.

Authors:

Reitsma, Sietze and oude Egbrink, Mirjam G A and Vink, Hans and van den Berg, Bernard M and Passos, Valéria Lima and Engels, Wim and Slaaf, Dick W and van Zandvoort, Marc A M J

Journal:

Journal of vascular research (2011): 297-306

Isolation and characterization of Saccharomyces cerevisiae mutants resistant to Calcofluor white.

Authors:

Roncero, C and Valdivieso, M H and Ribas, J C and Durán, A

Journal:

Journal of bacteriology (1988): 1950-4

Physical properties

Solvent

Water

Spectral properties

Absorbance (nm)	536
Correction factor (260 nm)	0.26
Correction factor (280 nm)	0.16
Extinction coefficient (cm ^-1 M ^-1)	90000 ¹
Excitation (nm)	537
Emission (nm)	560
Quantum yield	0.68 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Fluorescence microscope
Excitation	Cy3/TRITC filter set
Emission	Cy3/TRITC filter set
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 2, 2026