iFluor™ 546-streptavidin conjugate

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<p>The excitation and emission spectra of iFluor<sup>TM </sup>546. </p>
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Unit Size: Cat No: Price (USD): Qty:
200 ug 16958 $75


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Overview

Ex/Em (nm)541/557
SolventWater
Storage Freeze (<-15 °C)
Minimize light exposure
Category Immunology
Streptavidin Conjugates
Related
Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This iFluor™ 546-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with iFluor™ 546 covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. iFluor™ 546-streptavidin conjugates has fluorescence excitation and emission maxima essentially identical to those of Alexa Fluor® 546 conjugates. These spectral characteristics make it an excellent alternative to Alexa Fluor® 546-streptavidin conjugate (Alexa Fluor® is the trademark of Invitrogen). It is a very valuable tool for biotin-streptavidin-based biological assays and tests. Under the same conditions we tested, iFluor™ 546-streptavidin conjugate is brighter and more photostable than the corresponding Alexa Fluo® 546 conjugate. These spectral and labeling characteristics make the iFluor™ 546 dye family a superior alternative to Alexa Fluor® 546.




Spectrum Advanced Spectrum Viewer

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References & Citations

Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer
Authors: Hongyu Li, Hui Zhang, Shujun Zhao, Yun Shi, Junge Yao, Yanyan Zhang, Huanhuan Guo, Xingsuo Liu
Journal: Oncology letters (2015): 1989--1996






Additional Documents

 
Safety Data Sheet (SDS)


Catalogs
1. Fluorescent Labeling Probes & Kits

Certificate of Analysis