iFluor® 546-streptavidin conjugate
Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This iFluor® 546-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with iFluor® 546 covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. iFluor® 546-streptavidin conjugates has fluorescence excitation and emission maxima essentially identical to those of Alexa Fluor® 546 conjugates. These spectral characteristics make it an excellent alternative to Alexa Fluor® 546-streptavidin conjugate (Alexa Fluor® is the trademark of Invitrogen). It is a very valuable tool for biotin-streptavidin-based biological assays and tests. Under the same conditions we tested, iFluor® 546-streptavidin conjugate is brighter and more photostable than the corresponding Alexa Fluo® 546 conjugate. These spectral and labeling characteristics make the iFluor® 546 dye family a superior alternative to Alexa Fluor® 546.
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 350-streptavidin conjugate | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
iFluor® 405-streptavidin conjugate | 403 | 427 | 370001 | 0.911 | 0.48 | 0.77 |
iFluor® 488-streptavidin conjugate | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 514-streptavidin conjugate | 511 | 527 | 750001 | 0.831 | 0.265 | 0.116 |
iFluor® 532-streptavidin conjugate | 537 | 560 | 900001 | 0.681 | 0.26 | 0.16 |
iFluor® 555-streptavidin conjugate | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 594-streptavidin conjugate | 587 | 603 | 2000001 | 0.531 | 0.05 | 0.04 |
iFluor® 633-streptavidin conjugate | 640 | 654 | 2500001 | 0.291 | 0.062 | 0.044 |
iFluor® 647-streptavidin conjugate | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
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Citations
View all 1 citations: Citation Explorer
Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer
Authors: Li, Hongyu and Zhang, Hui and Zhao, Shujun and Shi, Yun and Yao, Junge and Zhang, Yanyan and Guo, Huanhuan and Liu, Xingsuo
Journal: Oncology letters (2015): 1989--1996
Authors: Li, Hongyu and Zhang, Hui and Zhao, Shujun and Shi, Yun and Yao, Junge and Zhang, Yanyan and Guo, Huanhuan and Liu, Xingsuo
Journal: Oncology letters (2015): 1989--1996
References
View all 47 references: Citation Explorer
A streptavidin paramagnetic-particle based competition assay for the evaluation of the optical selectivity of quadruplex nucleic acid fluorescent probes
Authors: Largy E, Hamon F, Teulade-Fichou MP.
Journal: Methods. (2012)
Authors: Largy E, Hamon F, Teulade-Fichou MP.
Journal: Methods. (2012)
Biotin-4-fluorescein based fluorescence quenching assay for determination of biotin binding capacity of streptavidin conjugated quantum dots
Authors: Mittal R, Bruchez MP.
Journal: Bioconjug Chem (2011): 362
Authors: Mittal R, Bruchez MP.
Journal: Bioconjug Chem (2011): 362
Iminobiotin binding induces large fluorescent enhancements in avidin and streptavidin fluorescent conjugates and exhibits diverging pH-dependent binding affinities
Authors: Raphael MP, Rappole CA, Kurihara LK, Christodoulides JA, Qadri SN, Byers JM.
Journal: J Fluoresc (2011): 647
Authors: Raphael MP, Rappole CA, Kurihara LK, Christodoulides JA, Qadri SN, Byers JM.
Journal: J Fluoresc (2011): 647
Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex
Authors: Kim JH, Chang TM, Graham AN, Choo KH, Kalitsis P, Hudson DF.
Journal: BMC Biochem (2010): 50
Authors: Kim JH, Chang TM, Graham AN, Choo KH, Kalitsis P, Hudson DF.
Journal: BMC Biochem (2010): 50
Determination of 17beta-oestradiol by fluorescence immunoassay with streptavidin-conjugated quantum dots as label
Authors: Sun M, Du L, Gao S, Bao Y, Wang S.
Journal: Steroids (2010): 400
Authors: Sun M, Du L, Gao S, Bao Y, Wang S.
Journal: Steroids (2010): 400
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