Name: iFluor® 546-streptavidin conjugate
Brand: AAT Bioquest
SKU: 16958
Price: 92 USD
Availability: InStock

iFluor® 546-streptavidin conjugate

iFluor® 546-Streptavidin Conjugate is an orange-fluorescent, biotin-binding probe for sensitive detection of biotinylated proteins, antibodies, nucleic acids, and other biomolecules in fluorescence-based assays.

High-Affinity Biotin Binding: Streptavidin enables specific detection of biotinylated targets.
Spectral Properties: Excitation at 541 nm and emission at 557 nm.
Enhanced Brightness: Manufacturer-reported brighter and more photostable fluorescence than comparable Alexa Fluor® 546-streptavidin conjugates.
Applications: Suitable for indirect immunofluorescence staining and biotin-streptavidin-based assays.

<p>The excitation and emission spectra of iFluor<sup>TM </sup>546. </p>

Datasheet

SDS

Catalog	Size	Price	Quantity
16958	200 ug	Price

Overview

Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This iFluor® 546-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with iFluor® 546 covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. iFluor® 546-streptavidin conjugates has fluorescence excitation and emission maxima essentially identical to those of Alexa Fluor® 546 conjugates. These spectral characteristics make it an excellent alternative to Alexa Fluor® 546-streptavidin conjugate (Alexa Fluor® is the trademark of Invitrogen). It is a very valuable tool for biotin-streptavidin-based biological assays and tests. Under the same conditions we tested, iFluor® 546-streptavidin conjugate is brighter and more photostable than the corresponding Alexa Fluo® 546 conjugate. These spectral and labeling characteristics make the iFluor® 546 dye family a superior alternative to Alexa Fluor® 546.

References

View all 47 references: Citation Explorer

A streptavidin paramagnetic-particle based competition assay for the evaluation of the optical selectivity of quadruplex nucleic acid fluorescent probes

Authors:

Largy E, Hamon F, Teulade-Fichou MP.

Journal:

Methods. (2012)

Biotin-4-fluorescein based fluorescence quenching assay for determination of biotin binding capacity of streptavidin conjugated quantum dots

Authors:

Mittal R, Bruchez MP.

Journal:

Bioconjug Chem (2011): 362

Iminobiotin binding induces large fluorescent enhancements in avidin and streptavidin fluorescent conjugates and exhibits diverging pH-dependent binding affinities

Authors:

Raphael MP, Rappole CA, Kurihara LK, Christodoulides JA, Qadri SN, Byers JM.

Journal:

J Fluoresc (2011): 647

Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex

Authors:

Kim JH, Chang TM, Graham AN, Choo KH, Kalitsis P, Hudson DF.

Journal:

BMC Biochem (2010): 50

Determination of 17beta-oestradiol by fluorescence immunoassay with streptavidin-conjugated quantum dots as label

Authors:

Sun M, Du L, Gao S, Bao Y, Wang S.

Journal:

Steroids (2010): 400

Physical properties

Molecular weight	~52000
Solvent	Water

Spectral properties

Correction factor (260 nm)	0.25
Correction factor (280 nm)	0.15
Extinction coefficient (cm ^-1 M ^-1)	100000 ¹
Excitation (nm)	541
Emission (nm)	557
Quantum yield	0.67 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 28, 2026