iFluor®555-PEG12-dUTP *1 mM in TE Buffer (pH 7.5)*

iFluor®555-PEG12-dUTP

1 mM in TE Buffer (pH 7.5)

The dye-modified deoxyuridine 5'-triphosphates are one of the most common methods to produce dye-labelled DNA via the conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This iFluor®555-PEG12-dUTP conjugate has the same spectral properties to ChromaTide™Alexa Fluor 555-dUTP. Its PEG12 spacer makes it much more effectively incorporated into DNAs by polymerase. It is used as a red fluorescence color with SpectrumOrange™ filter set. It provides a bright signal that has high photostability and is not affected by pH. ChromaTide™ and Alexa Fluor™ are the trademarks of ThermoFisher, and SpectrumOrange™ is the trademark of Vysis.

Fluorescence In Situ Hybridization of Cy3 and iFluor® 555-dUTP labelled Telomere probes in metaphase HeLa cells.

Protocol

SDS

Catalog	Size	Price	Quantity
17042	25 nmoles	Price

References

View all 48 references: Citation Explorer

A Fluorescent DNA Hydrogel Aptasensor Based on the Self-Assembly of Rolling Circle Amplification Products for Sensitive Detection of Ochratoxin A.

Authors:

Hao, Liling and Wang, Wei and Shen, Xueqing and Wang, Shuliu and Li, Qian and An, Faliang and Wu, Shijia

Journal:

Journal of agricultural and food chemistry (2020): 369-375

Engineering nicking enzymes that preferentially nick 5-methylcytosine-modified DNA.

Authors:

Gutjahr, Alice and Xu, Shuang-yong

Journal:

Nucleic acids research (2014): e77

Digital detection of multiple minority mutants in stool DNA for noninvasive colorectal cancer diagnosis.

Authors:

Deng, Lili and Qi, Zongtai and Zou, Binjie and Wu, Haiping and Huang, Huan and Kajiyama, Tomoharu and Kambara, Hideki and Zhou, Guohua

Journal:

Analytical chemistry (2012): 5645-52

Digital analysis of the expression levels of multiple colorectal cancer-related genes by multiplexed digital-PCR coupled with hydrogel bead-array.

Authors:

Qi, Zongtai and Ma, Yinjiao and Deng, Lili and Wu, Haiping and Zhou, Guohua and Kajiyama, Tomoharu and Kambara, Hideki

Journal:

The Analyst (2011): 2252-9

Microisolation and microcloning of bovine X-chromosomes for identification of sorted buffalo (Bubalus bubalis) spermatozoa.

Authors:

Zhuang, Xin-jie and Lu, Yang-qing and Zhang, Ming and Lu, Sheng-sheng and Lu, Ke-huan

Journal:

Animal reproduction science (2011): 32-6

Physical properties

Molecular weight	2454.14
Solvent	Water

Spectral properties

Correction factor (260 nm)	0.23
Correction factor (280 nm)	0.14
Extinction coefficient (cm ^-1 M ^-1)	100000 ¹
Excitation (nm)	557
Emission (nm)	570
Quantum yield	0.64 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Flyer: Labeled dUTPs

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Page updated on October 29, 2025