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iFluor®555-PEG12-dUTP
1 mM in TE Buffer (pH 7.5)
The dye-modified deoxyuridine 5'-triphosphates are one of the most common methods to produce dye-labelled DNA via the conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This iFluor®555-PEG12-dUTP conjugate has the same spectral properties to ChromaTide™Alexa Fluor 555-dUTP. Its PEG12 spacer makes it much more effectively incorporated into DNAs by polymerase. It is used as a red fluorescence color with SpectrumOrange™ filter set. It provides a bright signal that has high photostability and is not affected by pH. ChromaTide™ and Alexa Fluor™ are the trademarks of ThermoFisher, and SpectrumOrange™ is the trademark of Vysis.
Fluorescence In Situ Hybridization of Cy3 and iFluor® 555-dUTP labelled Telomere probes in metaphase HeLa cells.
Fluorescence In Situ Hybridization of Cy3 and iFluor® 555-dUTP labelled Telomere probes in metaphase HeLa cells.
CatalogSize
Price
Quantity
1704225 nmoles
Price
 
Physical properties

Molecular weight2454.14
SolventWater
Spectral properties

Correction factor (260 nm)0.23
Correction factor (280 nm)0.14
Extinction coefficient (cm -1 M -1)
100000
1
Excitation (nm)557
Emission (nm)570
Quantum yield
0.64
1
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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Page updated on October 3, 2025