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AAT Bioquest

iFluor® 555-streptavidin conjugate

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. A variety of the complementary biotinylated reagents are available from numerous commercial vendors.This iFluor® 555-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with iFluor® 555 covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. iFluor® 555-streptavidin conjugates has fluorescence excitation and emission maxima of ~559 nm and ~569 nm respectively. These spectral characteristics make it an excellent alternative to Alexa Fluor® 555-streptavidin conjugate (Alexa Fluor® is the trademark of Invitrogen). It is a very valuable tool for biotin-streptavidin-based biological assays and tests.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor® 350-streptavidin conjugate3454502000010.9510.830.23
iFluor® 405-streptavidin conjugate4034273700010.9110.480.77
iFluor® 488-streptavidin conjugate4915167500010.910.210.11
iFluor® 514-streptavidin conjugate5115277500010.8310.2650.116
iFluor® 532-streptavidin conjugate5375609000010.6810.260.16
iFluor® 594-streptavidin conjugate58760320000010.5310.050.04
iFluor® 633-streptavidin conjugate64065425000010.2910.0620.044
iFluor® 647-streptavidin conjugate65667025000010.2510.030.03
iFluor® 680-streptavidin conjugate68470122000010.2310.0970.094
iFluor® 700-streptavidin conjugate69071322000010.2310.090.04
iFluor® 750-streptavidin conjugate75777927500010.1210.0440.039
iFluor® 546-streptavidin conjugate54155710000010.6710.250.15
iFluor® 568-streptavidin conjugate56858710000010.5710.340.15
iFluor® 790-streptavidin conjugate78781225000010.1310.10.09
iFluor® 800-streptavidin conjugate80182025000010.1110.030.08
iFluor® 820-streptavidin conjugate82285025000010.110.16
iFluor® 840-streptavidin conjugate8368792000001-0.20.09
iFluor® 860-streptavidin conjugate85387825000010.10.14
Phalloidin-iFluor® 555 Conjugate55757010000010.6410.230.14
Show More (10)

Citations

View all 4 citations: Citation Explorer
An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress
Authors: N{\"u}chel, Julian and Tauber, Marina and Nolte, Janica L and M{\"o}rgelin, Matthias and T{\"u}rk, Clara and Eckes, Beate and Demetriades, Constantinos and Plomann, Markus
Journal: Molecular cell (2021): 3275--3293
Prostaglandin A1 Decreases the Phosphorylation of Tau by Activating Protein Phosphatase 2A via a Michael Addition Mechanism at Cysteine 377
Authors: Xu, Guo-Biao and Guan, Pei-Pei and Wang, Pu
Journal: Molecular Neurobiology (2020): 1--14
Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer
Authors: Li, Hongyu and Zhang, Hui and Zhao, Shujun and Shi, Yun and Yao, Junge and Zhang, Yanyan and Guo, Huanhuan and Liu, Xingsuo
Journal: Oncology letters (2015): 1989--1996

References

View all 47 references: Citation Explorer
A streptavidin paramagnetic-particle based competition assay for the evaluation of the optical selectivity of quadruplex nucleic acid fluorescent probes
Authors: Largy E, Hamon F, Teulade-Fichou MP.
Journal: Methods. (2012)
Biotin-4-fluorescein based fluorescence quenching assay for determination of biotin binding capacity of streptavidin conjugated quantum dots
Authors: Mittal R, Bruchez MP.
Journal: Bioconjug Chem (2011): 362
Iminobiotin binding induces large fluorescent enhancements in avidin and streptavidin fluorescent conjugates and exhibits diverging pH-dependent binding affinities
Authors: Raphael MP, Rappole CA, Kurihara LK, Christodoulides JA, Qadri SN, Byers JM.
Journal: J Fluoresc (2011): 647
Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex
Authors: Kim JH, Chang TM, Graham AN, Choo KH, Kalitsis P, Hudson DF.
Journal: BMC Biochem (2010): 50
Multimodality nuclear and fluorescence tumor imaging in mice using a streptavidin nanoparticle
Authors: Liang M, Liu X, Cheng D, Liu G, Dou S, Wang Y, Rusckowski M, Hnatowich DJ.
Journal: Bioconjug Chem (2010): 1385
Page updated on October 9, 2024

Ordering information

Price
Unit size
200 ug
1 mg
Catalog Number
Quantity
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Additional ordering information

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Physical properties

Molecular weight

~52000

Solvent

Water

Spectral properties

Correction Factor (260 nm)

0.23

Correction Factor (280 nm)

0.14

Extinction coefficient (cm -1 M -1)

1000001

Excitation (nm)

557

Emission (nm)

570

Quantum yield

0.641

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Image of HeLa cells. HeLa cells were incubated with (Tubulin+) or without (Control) rabbit anti-tubulin, followed by biotinylated goat anti-rabbit IgG (H&amp;L) (Cat# 16794) stain, and finally visualized with iFluor® 555-streptavidin conjugate (Cat# 16959).
Image of HeLa cells. HeLa cells were incubated with (Tubulin+) or without (Control) rabbit anti-tubulin, followed by biotinylated goat anti-rabbit IgG (H&amp;L) (Cat# 16794) stain, and finally visualized with iFluor® 555-streptavidin conjugate (Cat# 16959).
Image of HeLa cells. HeLa cells were incubated with (Tubulin+) or without (Control) rabbit anti-tubulin, followed by biotinylated goat anti-rabbit IgG (H&amp;L) (Cat# 16794) stain, and finally visualized with iFluor® 555-streptavidin conjugate (Cat# 16959).
HeLa cells were incubated with mouse anti-tubulin and biotin goat anti-mouse IgG followed by AAT&rsquo;s iFluor® 555-streptavidin conjugate (Red). Cell nuclei were stained with Hoechst 33342 (Blue).