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iFluor® 555-Wheat Germ Agglutinin (WGA) Conjugate
Wheat germ agglutinin (WGA) is a lectin that binds to N-acetyl-D-glucosamine and sialic acid. It is of the most studied and useful lectins for it biological applications. Since WGA binds to glycoconjugates its derivatives and conjugates are widely used to label cell membranes and fibrotic scar tissue for fluorescence imaging and analysis. The carbohydrate-binding specificity of WGA is directed against sequences of β-1,4-GlcNAc-linked residues, the chitodextrins. Each monomer contains two identical, non-interacting binding sites which are complementary to 3 or 4 β-1,4-GlcNAc units. Of the monosaccharides examined, only GlcNAc binds to WGA. ManNAc does not bind and GalNAc binds only weakly. It exhibits the bright, red fluorescence of the iFluor® 555 dye. iFluor®555 WGA conjugate binds to sialic acid and N-acetylglucosaminyl residues as AF555 WGA conjugate does.
Live HeLa cells were stained with iFluor® 555-Wheat Germ Agglutinin (WGA) Conjugate at 5 µg/mL for 30 minutes followed by Hoechst 33342 (AAT Cat# 17535). Image was acquired using fluorescence microscopy using Cy3/TRITC and DAPI filter set.
Live HeLa cells were stained with iFluor® 555-Wheat Germ Agglutinin (WGA) Conjugate at 5 µg/mL for 30 minutes followed by Hoechst 33342 (AAT Cat# 17535). Image was acquired using fluorescence microscopy using Cy3/TRITC and DAPI filter set.
CatalogSize
Price
Quantity
255391 mg
Price
 
Physical properties

SolventWater
Spectral properties

Correction factor (260 nm)0.23
Correction factor (280 nm)0.14
Extinction coefficient (cm -1 M -1)
100000
1
Excitation (nm)557
Emission (nm)570
Quantum yield
0.64
1
Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Instrument settings

Fluorescence microscope
ExcitationCy3/TRITC filter set
EmissionCy3/TRITC filter set
Recommended plateBlack wall/clear bottom
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Page updated on October 8, 2024