Wheat Germ Agglutinin (WGA)

iFluor® 555-Wheat Germ Agglutinin (WGA) Conjugate

iFluor® 555-Wheat Germ Agglutinin (WGA) Conjugate is an orange-fluorescent lectin probe for detecting cell-surface glycoconjugates and visualizing cell membranes in live and fixed cells.

Selective Glycoconjugate Binding: Recognizes N-acetylglucosamine and sialic acid residues.
Spectral Properties: Excitation at 557 nm and emission at 570 nm.
Cell Membrane Labeling: Membrane-impermeant probe enables cell-surface staining of intact cells.
Filter Compatibility: Supports fluorescence imaging using standard Cy3/TRITC filter sets.
Applications: Suitable for live- and fixed-cell imaging, with potential intracellular staining following permeabilization.

Live HeLa cells were stained with iFluor® 555-Wheat Germ Agglutinin (WGA) Conjugate at 5 µg/mL for 30 minutes followed by Hoechst 33342 (AAT Cat# 17535). Image was acquired using fluorescence microscopy using Cy3/TRITC and DAPI filter set.

Catalog	Size	Price	Quantity
25539	1 mg	Price

Overview

Wheat germ agglutinin (WGA) is a lectin that binds to N-acetyl-D-glucosamine and sialic acid. It is of the most studied and useful lectins for it biological applications. Since WGA binds to glycoconjugates its derivatives and conjugates are widely used to label cell membranes and fibrotic scar tissue for fluorescence imaging and analysis. The carbohydrate-binding specificity of WGA is directed against sequences of β-1,4-GlcNAc-linked residues, the chitodextrins. Each monomer contains two identical, non-interacting binding sites which are complementary to 3 or 4 β-1,4-GlcNAc units. Of the monosaccharides examined, only GlcNAc binds to WGA. ManNAc does not bind and GalNAc binds only weakly. It exhibits the bright, red fluorescence of the iFluor® 555 dye. iFluor®555 WGA conjugate binds to sialic acid and N-acetylglucosaminyl residues as AF555 WGA conjugate does.

Citations

View all 6 citations: Citation Explorer

GNPs-pIL-4 reprograms macrophage polarization and activates the OSM/GSNOR/ENG axis to improve angiogenesis in ischemic limbs

Authors:

Zhang, Peng and Zhuang, Jinman and Hao, Yizhou and Zhu, Xiangrui and Liang, Hongbiao and Hu, Guizimeng and Li, Peiyi and Song, Yuwei and Liu, Qijia and Yang, Guangxin and others

Journal:

Science Advances (2026): eaed5692

PPP2R3A-RGS19 interaction links Wnt/$\beta$-catenin signaling to pathological cardiomyocyte apoptosis in heart failure

Authors:

Qin, Shuang and Su, Lingge and Gao, Jing and Wu, Chunyan and Zhang, Yating and Shi, Hongqiong and Song, Guibo

Journal:

Molecular Biology Reports (2026): 651

Deciphering the Role of Sarcospan in Cardiovascular Disease

Authors:

Kahmini, Aida Rahimi

Aging Reveals a Sex-Dependent Susceptibility of Sarcospan-Deficient Mice to Cardiometabolic Disease

Authors:

Rahimi Kahmini, Aida and Valera, Isela C and Crawford, Rhiannon Q and Samarah, Luaye and Reis, Gisienne and Elsheikh, Salma and Kanashiro-Takeuchi, Rosemeire M and Mohammadipoor, Nazanin and Olateju, Bolade S and Matthews, Aaron R and others

Journal:

American Journal of Physiology-Heart and Circulatory Physiology (2024)

Transformable nano-antibiotics for mechanotherapy and immune activation against drug-resistant Gram-negative bacteria

Authors:

Li, Rong Sheng and Liu, Jiahui and Wen, Cong and Shi, Yaru and Ling, Jian and Cao, Qiue and Wang, Lei and Shi, Hu and Huang, Cheng Zhi and Li, Na

Journal:

Science Advances (2023): eadg9601

References

View all 49 references: Citation Explorer

Wheat germ agglutinin is involved in the protective action of 24-epibrassinolide on the roots of wheat seedlings under drought conditions.

Authors:

Avalbaev, Azamat and Bezrukova, Marina and Allagulova, Chulpan and Lubyanova, Alsu and Kudoyarova, Guzel and Fedorova, Kristina and Maslennikova, Dilara and Yuldashev, Ruslan and Shakirova, Farida

Journal:

Plant physiology and biochemistry : PPB (2020): 420-427

Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men.

Authors:

Janković, Tamara and Goč, Sanja and Mitić, Ninoslav and Danilović Luković, Jelena and Janković, Miroslava

Journal:

Upsala journal of medical sciences (2020): 10-18

Succinylated Wheat Germ Agglutinin Colocalizes with the Toxoplasma gondii Cyst Wall Glycoprotein CST1.

Authors:

Guevara, Rebekah B and Fox, Barbara A and Bzik, David J

Journal:

mSphere (2020)

Wheat germ agglutinin is a biomarker of whole grain content in wheat flour and pasta.

Authors:

Killilea, David W and McQueen, Rebecca and Abegania, Judi R

Journal:

Journal of food science (2020): 808-815

Wheat germ agglutinin liposomes with surface grafted cyclodextrins as bioadhesive dual-drug delivery nanocarriers to treat oral cells.

Authors:

Wijetunge, Sashini S and Wen, Jianchuan and Yeh, Chih-Ko and Sun, Yuyu

Journal:

Colloids and surfaces. B, Biointerfaces (2020): 110572

Physical properties

Solvent

Water

Spectral properties

Correction factor (260 nm)	0.23
Correction factor (280 nm)	0.14
Extinction coefficient (cm ^-1 M ^-1)	100000 ¹
Excitation (nm)	557
Emission (nm)	570
Quantum yield	0.64 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Fluorescence microscope
Excitation	Cy3/TRITC filter set
Emission	Cy3/TRITC filter set
Recommended plate	Black wall/clear bottom

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Page updated on October 2, 2026