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iFluor® 568-streptavidin conjugate

HeLa cells were incubated with mouse anti-tubulin followed with iFluor<sup>TM</sup> 568 goat anti-mouse IgG conjugate (Red). Cell nuclei were stained with DAPI (Blue, Cat#17507).
HeLa cells were incubated with mouse anti-tubulin followed with iFluor<sup>TM</sup> 568 goat anti-mouse IgG conjugate (Red). Cell nuclei were stained with DAPI (Blue, Cat#17507).
HeLa cells were incubated with mouse anti-tubulin followed with iFluor<sup>TM</sup> 568 goat anti-mouse IgG conjugate (Red). Cell nuclei were stained with DAPI (Blue, Cat#17507).
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Physical properties
Molecular weight~52000
SolventWater
Spectral properties
Correction Factor (260 nm)0.34
Correction Factor (280 nm)0.15
Extinction coefficient (cm -1 M -1)1000001
Excitation (nm)568
Emission (nm)587
Quantum yield0.571
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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iFluor® 514 goat anti-mouse IgG (H+L)
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iFluor® 633 goat anti-mouse IgG (H+L)
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iFluor® 700 goat anti-mouse IgG (H+L)
iFluor® 750 goat anti-mouse IgG (H+L)
iFluor® 790 goat anti-mouse IgG (H+L)
iFluor® 350 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 405 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 488 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 514 goat anti-mouse IgG (H+L) *Cross Adsorbed*
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iFluor® 647 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 680 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 700 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 750 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 790 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 350 goat anti-rabbit IgG (H+L)
iFluor® 405 goat anti-rabbit IgG (H+L)
iFluor® 488 goat anti-rabbit IgG (H+L)
iFluor® 514 goat anti-rabbit IgG (H+L)
iFluor® 532 goat anti-rabbit IgG (H+L)
iFluor® 555 goat anti-rabbit IgG (H+L)
iFluor® 594 goat anti-rabbit IgG (H+L)
iFluor® 633 goat anti-rabbit IgG (H+L)
iFluor® 647 goat anti-rabbit IgG (H+L)
iFluor® 680 goat anti-rabbit IgG (H+L)
iFluor® 700 goat anti-rabbit IgG (H+L)
iFluor® 750 goat anti-rabbit IgG (H+L)
iFluor® 790 goat anti-rabbit IgG (H+L)
iFluor® 350 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 405 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 488 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 514 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
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iFluor® 680 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 700 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
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iFluor® 568 goat anti-mouse IgG (H+L)
iFluor® 568 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 546 goat anti-rabbit IgG (H+L)
iFluor® 546 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 568 goat anti-rabbit IgG (H+L)
iFluor® 568 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 555 alkyne
iFluor® 555 azide
iFluor® 532 maleimide
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iFluor® 594 PSA™ Imaging Kit with Goat Anti-Rabbit IgG
iFluor® 647 PSA™ Imaging Kit with Goat Anti-Rabbit IgG
iFluor® 350 PSA™ Imaging Kit with Goat Anti-Mouse IgG
iFluor® 488 PSA™ Imaging Kit with Goat Anti-Mouse IgG
iFluor® 555 PSA™ Imaging Kit with Goat Anti-Mouse IgG
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iFluor® 810 goat anti-rabbit IgG (H+L)
iFluor® 810 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
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iFluor® 860 goat anti-rabbit IgG (H+L)
iFluor® 860 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
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iFluor® 450 Tyramide *Superior Replacement for Opal 480*
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iFluor® 840 maleimide
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iFluor® 780 maleimide
iFluor® 350 succinimidyl ester
iFluor® 405 succinimidyl ester
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iFluor® 514 succinimidyl ester
iFluor® 532 succinimidyl ester
iFluor® 555 succinimidyl ester
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iFluor® 633 succinimidyl ester
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iFluor® 660 succinimidyl ester
iFluor® 680 succinimidyl ester
iFluor® 700 succinimidyl ester
iFluor® 750 succinimidyl ester
iFluor® 610 succinimidyl ester
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iFluor® 790 succinimidyl ester
iFluor® 800 succinimidyl ester
iFluor® 810 succinimidyl ester
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iFluor® 568 succinimidyl ester
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iFluor® 450 succinimidyl ester
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iFluor® 720 succinimidyl ester
iFluor® 740 succinimidyl ester
iFluor® 597 succinimidyl ester
iFluor® 770 succinimidyl ester
iFluor® 780 succinimidyl ester
iFluor® 570 succinimidyl ester
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iFluor® 625 maleimide
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iFluor® 540 succinimidyl ester
iFluor® 540 maleimide
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iFluor® 532-Wheat Germ Agglutinin (WGA) Conjugate
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iFluor® 700-Wheat Germ Agglutinin (WGA) Conjugate
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FITC-streptavidin conjugate
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mFluor™ Violet 450-streptavidin conjugate
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mFluor™ Red 700-streptavidin conjugate
mFluor™ Red 780-streptavidin conjugate
XFD350-streptavidin conjugate *XFD350 Same Structure to Alexa Fluor™ 350*
XFD488-streptavidin conjugate *XFD488 Same Structure to Alexa Fluor™ 488*
XFD594-streptavidin conjugate *XFD594 Same Structure to Alexa Fluor™ 594*
RPE-Cy7-streptavidin conjugate
MegaWox™ polyHRP-Streptavidin Conjugate
RPE-Cy5-streptavidin conjugate
mFluor™ UV455-streptavidin conjugate
mFluor™ Violet 545-streptavidin conjugate
mFluor™ Violet 550-streptavidin conjugate
mFluor™ Violet 590-streptavidin conjugate
mFluor™ Violet 610-streptavidin conjugate
mFluor™ UV420-streptavidin conjugate
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OverviewpdfSDSpdfProtocol


Molecular weight
~52000
Correction Factor (260 nm)
0.34
Correction Factor (280 nm)
0.15
Extinction coefficient (cm -1 M -1)
1000001
Excitation (nm)
568
Emission (nm)
587
Quantum yield
0.571
Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This iFluor® 568-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with iFluor® 568 covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. iFluor® 568-streptavidin conjugates has fluorescence excitation and emission maxima essentially identical to those of Alexa Fluor® 568 conjugates. These spectral characteristics make it an excellent alternative to Alexa Fluor® 568-streptavidin conjugate (Alexa Fluor® is the trademark of Invitrogen). It is a very valuable tool for biotin-streptavidin-based biological assays and tests. Under the same conditions we tested, iFluor® 568-streptavidin conjugate is brighter and more photostable than the corresponding Alexa Fluo® 568 conjugate. These spectral and labeling characteristics make the iFluor® 568 dye family a superior alternative to Alexa Fluor® 568.

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Correction Factor (260 nm)0.34
Correction Factor (280 nm)0.15
Extinction coefficient (cm -1 M -1)1000001
Excitation (nm)568
Emission (nm)587
Quantum yield0.571

Product Family


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iFluor® 514-streptavidin conjugate5115277500010.8310.2650.116
iFluor® 532-streptavidin conjugate5375609000010.6810.260.16
iFluor® 555-streptavidin conjugate55757010000010.6410.230.14
iFluor® 594-streptavidin conjugate58760320000010.5310.050.04
iFluor® 633-streptavidin conjugate64065425000010.2910.0620.044
iFluor® 647-streptavidin conjugate65667025000010.2510.030.03
iFluor® 680-streptavidin conjugate68470122000010.2310.0970.094
iFluor® 700-streptavidin conjugate69071322000010.2310.090.04
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iFluor® 546-streptavidin conjugate54155710000010.6710.250.15
iFluor® 790-streptavidin conjugate78781225000010.1310.10.09
iFluor® 800-streptavidin conjugate80182025000010.1110.030.08
iFluor® 820-streptavidin conjugate82285025000010.110.16
iFluor® 840-streptavidin conjugate8368792000001-0.20.09
iFluor® 860-streptavidin conjugate85387825000010.10.14
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Images


Citations


View all 1 citations: Citation Explorer
Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer
Authors: Li, Hongyu and Zhang, Hui and Zhao, Shujun and Shi, Yun and Yao, Junge and Zhang, Yanyan and Guo, Huanhuan and Liu, Xingsuo
Journal: Oncology letters (2015): 1989--1996

References


View all 47 references: Citation Explorer
A streptavidin paramagnetic-particle based competition assay for the evaluation of the optical selectivity of quadruplex nucleic acid fluorescent probes
Authors: Largy E, Hamon F, Teulade-Fichou MP.
Journal: Methods. (2012)
Biotin-4-fluorescein based fluorescence quenching assay for determination of biotin binding capacity of streptavidin conjugated quantum dots
Authors: Mittal R, Bruchez MP.
Journal: Bioconjug Chem (2011): 362
Iminobiotin binding induces large fluorescent enhancements in avidin and streptavidin fluorescent conjugates and exhibits diverging pH-dependent binding affinities
Authors: Raphael MP, Rappole CA, Kurihara LK, Christodoulides JA, Qadri SN, Byers JM.
Journal: J Fluoresc (2011): 647
Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex
Authors: Kim JH, Chang TM, Graham AN, Choo KH, Kalitsis P, Hudson DF.
Journal: BMC Biochem (2010): 50
Determination of 17beta-oestradiol by fluorescence immunoassay with streptavidin-conjugated quantum dots as label
Authors: Sun M, Du L, Gao S, Bao Y, Wang S.
Journal: Steroids (2010): 400
Multimodality nuclear and fluorescence tumor imaging in mice using a streptavidin nanoparticle
Authors: Liang M, Liu X, Cheng D, Liu G, Dou S, Wang Y, Rusckowski M, Hnatowich DJ.
Journal: Bioconjug Chem (2010): 1385
Site-dependent excited-state dynamics of a fluorescent probe bound to avidin and streptavidin
Authors: Furstenberg A, Kel O, Gradinaru J, Ward TR, Emery D, Bollot G, Mareda J, Vauthey E.
Journal: Chemphyschem (2009): 1517
Influence of streptavidin on the absorption and fluorescence properties of cyanine dyes
Authors: Luschtinetz F, Dosche C, Kumke MU.
Journal: Bioconjug Chem (2009): 576
Fluorescent nanoscale detection of biotin-streptavidin interaction using near-field scanning optical microscopy
Authors: Park HK, Gokarna A, Hulme JP, Park HG, Chung BH.
Journal: Nanotechnology (2008): 235103
Application of biotin-4-fluorescein in homogeneous fluorescence assays for avidin, streptavidin, and biotin or biotin derivatives
Authors: Ebner A, Marek M, Kaiser K, Kada G, Hahn CD, Lackner B, Gruber HJ.
Journal: Methods Mol Biol (2008): 73