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iFluor® 568-streptavidin conjugate
Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This iFluor® 568-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with iFluor® 568 covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. iFluor® 568-streptavidin conjugates has fluorescence excitation and emission maxima essentially identical to those of Alexa Fluor® 568 conjugates. These spectral characteristics make it an excellent alternative to Alexa Fluor® 568-streptavidin conjugate (Alexa Fluor® is the trademark of Invitrogen). It is a very valuable tool for biotin-streptavidin-based biological assays and tests. Under the same conditions we tested, iFluor® 568-streptavidin conjugate is brighter and more photostable than the corresponding Alexa Fluo® 568 conjugate. These spectral and labeling characteristics make the iFluor® 568 dye family a superior alternative to Alexa Fluor® 568.
HeLa cells were incubated with mouse anti-tubulin followed with iFluor<sup>TM</sup> 568 goat anti-mouse IgG conjugate (Red). Cell nuclei were stained with DAPI (Blue, Cat#17507).
HeLa cells were incubated with mouse anti-tubulin followed with iFluor<sup>TM</sup> 568 goat anti-mouse IgG conjugate (Red). Cell nuclei were stained with DAPI (Blue, Cat#17507).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
16960200 ug
Price
 
Physical properties
Molecular weight~52000
SolventWater
Spectral properties
Correction factor (260 nm)0.34
Correction factor (280 nm)0.15
Extinction coefficient (cm -1 M -1)
100000
1
Excitation (nm)568
Emission (nm)587
Quantum yield
0.57
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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Page updated on October 12, 2025