Name: iFluor® 570-dUTP *1 mM in TE Buffer (pH 7.5)*
Brand: AAT Bioquest
SKU: 17048
Price: 118 USD
Availability: InStock

iFluor® 570-dUTP

1 mM in TE Buffer (pH 7.5)

iFluor® 570-dUTP is a fluorescently modified nucleotide that incorporates into DNA during enzymatic synthesis, producing bright orange-labeled probes for FISH, microarray hybridization, and nucleic acid detection.

Broad Labeling Compatibility: Supports diverse techniques like reverse transcription, PCR, and nick translation
Superior Stability: Enhanced photostability vs. Alexa Fluor® 546-dNTPs
pH Resilient: Stable fluorescence in pH up to 10, ideal for DNA sequencing
Bright Yellow Signal: Compatible with SpectrumOrange™ filters

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17048	25 nmoles	Price

Overview

iFluor® 570-modified deoxyuridine 5'-triphosphates are widely used for the production of dye-labeled DNA via conventional enzymatic incorporation techniques, including reverse transcription, nick translation, random primed labeling, and PCR. These enzymatic fluorescence labeling methods are commonly employed in both fluorescence in situ hybridization (FISH) probes and microarray-based experiments. The iFluor® 570-dUTP conjugate, which emits yellow fluorescence, is compatible with the SpectrumOrange™ filter set (SpectrumOrange™ is a trademark of Vysis). Compared to Alexa Fluor® 546-labeled dNTPs, iFluor® 570 offers a significantly brighter signal, enhanced photostability, and greater resilience to pH fluctuations, maintaining stability even in higher pH conditions (e.g., pH 8–10, commonly used in DNA sequencing).

References

View all 50 references: Citation Explorer

The impact of crowding stress on growth and intestinal integrity in largemouth bass (Micropterus salmoides): Insights into ER stress, autophagy and apoptosis.

Authors:

Yan, Dadong and Gan, Lei and Dong, Xianghong and Tie, Huaimao and Luo, Chao and Wang, Zhenlu and Jiang, Haibo and Chen, Jiangfeng and An, Miao and Qin, Chuanjie and Lu, Zhiyuan

Journal:

Fish & shellfish immunology (2024): 109955

Hepatic melanomacrophage centers in the arctic cultured fish Cyclopterus lumpus are not indicative of its health state.

Authors:

Passantino, L and Corriero, A and Pousis, C and Zupa, R and Perillo, A and Superio, J and Kumari Swain, J and Foss, A and Galindo-Villegas, J and Ventriglia, G

Journal:

Aquaculture (Amsterdam, Netherlands) (2024): 740417

Immune response and apoptosis of gibel carp (Carassius auratus gibelio) gills to Chilodonella hexasticha infection: Insights from histopathological and multi-omics analyses.

Authors:

Bu, Xialian and Zhao, Weishan and Zou, Hong and Li, Wenxiang and Li, Ming and Wang, Guitang

Journal:

Fish & shellfish immunology (2024): 109429

Biomarkers of growth and carbohydrate metabolism in neonatal rats supplemented with fish oil and/or antioxidants during intermittent hypoxia.

Authors:

Galetaki, Despoina Myrsini and Cai, Charles L and Bhatia, Kulsajan S and Chin, Vivian and Aranda, Jacob V and Beharry, Kay D

Journal:

Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Socie (2023): 101513

Ginsenoside Rg1 alleviates sleep deprivation-induced learning and memory impairment by inhibiting excessive neuronal apoptosis in zebrafish.

Authors:

Lu, Haifei and Zhang, Yini and Ran, Simiao and Chen, Yumeng and Ye, Zijing and Huang, Mengying and Wang, Ping

Journal:

Neuroreport (2023): 566-574

Physical properties

Molecular weight	1435.30
Solvent	Water

Spectral properties

Correction factor (260 nm)	0.049
Correction factor (280 nm)	0.074
Extinction coefficient (cm ^-1 M ^-1)	120000 ¹
Excitation (nm)	557
Emission (nm)	570
Quantum yield	0.71 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

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Custom size	Inquire
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Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 13, 2025