Name: iFluor® 570 Styramide
Brand: AAT Bioquest
SKU: 45031
Price: 355 USD
Availability: InStock

iFluor® 570 Styramide

The Power Styramide™ Signal Amplification (PSA™) system is a highly sensitive method for detecting low-abundance targets in cells and tissues, with fluorescence signals 10-50 times higher than tyramide (TSA) reagents. Paired with our iFluor® dyes, known for their fluorescence intensity, photostability, and water solubility, the iFluor® dye-labeled Styramide™ conjugates achieve precision and sensitivity surpassing standard ICC/IF/IHC methods by over 100 times. PSA™ relies on horseradish peroxidase (HRP) catalytic activity to covalently deposit fluorophores in situ, with radicals displaying higher reactivity than tyramide radicals, making the PSA™ system faster, more robust, and more sensitive than traditional TSA reagents. The Styramide™ conjugates label targets with higher efficiency, leading to significantly greater fluorescence signals while also enabling reduced primary antibody consumption compared to standard methods. iFluor® 570 Styramide is a replacement for Alexa Fluor™ 568 tyramide and similar fluorescent tyramide conjugates or TSA reagents, offering unmatched performance in sensitive imaging applications.

Fluorescence IHC was performed on formaldehyde-fixed, paraffin-embedded human lung adenocarcinoma-positive tissue using PSA™ and TSA amplified methods. First, the tissue sections were stained with rabbit anti-EpCam antibody and then incubated with polyHRP-labeled Goat anti-Rabbit IgG secondary antibody. The signal was developed using either iFluor® 570 Styramide™ (Cat No. 45031) or Alexa Fluor® 568 tyramide stain, respectively, and detected with a Cy3/TRITC filter set. Finally, the nuclei (blue) were counterstained with DAPI (Cat No. 17507).

Protocol

SDS

Catalog	Size	Price	Quantity
45031	100 Slides	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 570 Styramide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	94.131 µL	470.655 µL	941.309 µL	4.707 mL	9.413 mL
5 mM	18.826 µL	94.131 µL	188.262 µL	941.309 µL	1.883 mL
10 mM	9.413 µL	47.065 µL	94.131 µL	470.655 µL	941.309 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1062.35 g/mol	=	mL	x	mM	=	mmol

References

View all 4 references: Citation Explorer

Multiplex Fluorescent Immunohistochemistry for Preservation of Tumor Microenvironment Architecture and Spatial Relationship of Cells in Tumor Tissues.

Authors:

McGue, Jake J and Edwards, Jacob J and Griffith, Brian D and Frankel, Timothy L

Journal:

Methods in molecular biology (Clifton, N.J.) (2023): 235-246

Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry.

Authors:

Quigley, Luke T and Pang, Lokman and Tavancheh, Elnaz and Ernst, Matthias and Behren, Andreas and Huynh, Jennifer and Da Gama Duarte, Jessica

Journal:

STAR protocols (2023): 101961

Multiplex Immunohistochemistry Analysis of Melanoma Tumor-Infiltrating Lymphocytes.

Authors:

Nguyen, Thu and Kocovski, Nikolce and Macdonald, Sean and Yeang, Han Xian Aw and Wang, Minyu and Neeson, Paul J

Journal:

Methods in molecular biology (Clifton, N.J.) (2021): 557-572

Mixed Multiplex Staining: Automated RNAscope™ and OPAL™ for Multiple Targets.

Authors:

Millar, Michael

Journal:

Methods in molecular biology (Clifton, N.J.) (2020): 277-298

Physical properties

Molecular weight	1062.35
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.049
Correction factor (280 nm)	0.074
Extinction coefficient (cm ^-1 M ^-1)	120000 ¹
Excitation (nm)	557
Emission (nm)	570
Quantum yield	0.71 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Instrument settings

Fluorescence microscope
Excitation	Cy3/TRITC filter set
Emission	Cy3/TRITC filter set
Recommended plate	Black wall/clear bottom

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Page updated on April 11, 2026