Name: iFluor® 594 PSA™ Imaging Kit with Goat Anti-Mouse IgG
Brand: AAT Bioquest
SKU: 45280
Price: 594 USD
Availability: InStock

iFluor® 594 PSA™ Imaging Kit with Goat Anti-Mouse IgG

iFluor® 594 PSA™ Imaging Kit with Goat Anti-Mouse IgG is a fluorescence-based signal amplification kit designed for sensitive detection of low-abundance targets in cells and tissues using mouse primary antibodies.

Detection Principle: Uses HRP-mediated Styramide™ deposition for fluorescence signal amplification.
High Sensitivity: Generates 10–50 times stronger signals than conventional tyramide reagents.
Spectral Properties: Excitation/emission maxima of 587/603 nm.
Applications: Suitable for immunocytochemistry, immunofluorescence, and immunohistochemistry.

Fluorescence IHC of formaldehyde-fixed, paraffin-embedded using PSA<strong> ™ </strong> and TSA amplified methods. Human lung adenocarcinoma positive tissue sections were stained with mouse anti-EpCam antibody and then followed by PSA™ method using iFluor 594™ PSA™ Imaging Kit with Goat Anti-Mouse IgG (Cat#45280) or TSA method using  Alexa Fluor® 594 tyramide  respectively.  Images showed that PSA™ super signal amplification can increase the sensitivity of fluorescence IHC over Alexa Fluor® 594 TSA method. Cell nucleus were stained with Nuclear Blue™ DCS1 (Cat#17548).

Protocol

SDS

Catalog	Size	Price	Quantity
45280	100 Tests	Price

Overview

Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. In combination with our superior iFluor® dyes that have higher florescence intensity, increased photostability and enhanced water solubility, the iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA™ utilizes the catalytic activity of horseradish peroxidase (HRP) for covalent deposition of fluorophores in situ. PSA™ radicals have much higher reactivity than tyramide radicals, making the PSA™ system much faster, more robust and sensitive than the traditional TSA reagents. Compared to tyramide reagents, the Styramide™ conjugates have ability to label the target at higher efficiency and thus generate significantly higher fluorescence signal. Styramide™ conjugates also allow significantly less consumption of primary antibody compared to standard directly conjugate method or tyramide amplification with the same level of sensitivity. iFluor® 594 PSA™ kit is a much superior replacement for Alexa Fluor 594 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.

Spectral properties

Absorbance (nm)	587
Correction factor (260 nm)	0.05
Correction factor (280 nm)	0.04
Extinction coefficient (cm ^-1 M ^-1)	200000 ¹
Excitation (nm)	587
Emission (nm)	603
Quantum yield	0.53 ¹

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microscope
Excitation	Cy3/TRITC filter set
Emission	Cy3/TRITC filter set
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 28, 2026

Component A: iFluor® 594 Styramide™ conjugate	1 vial (lyophilized powder)
Component B: Styramide™ Reaction Buffer	1 bottle (10 mL)
Component C: DMSO	1 vial (100 µL)
Component D: Secondary Antibody-HRP (Goat Anti-Mouse IgG-HRP)	1 vial (100 µL) (100X)
Component E: Stabilized 3% Hydrogen Peroxide (H2O2)	1 vial (200 µL)