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iFluor® 594 PSA™ Imaging Kit with Goat Anti-Mouse IgG
Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. In combination with our superior iFluor® dyes that have higher florescence intensity, increased photostability and enhanced water solubility, the iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA™ utilizes the catalytic activity of horseradish peroxidase (HRP) for covalent deposition of fluorophores in situ. PSA™ radicals have much higher reactivity than tyramide radicals, making the PSA™ system much faster, more robust and sensitive than the traditional TSA reagents. Compared to tyramide reagents, the Styramide™ conjugates have ability to label the target at higher efficiency and thus generate significantly higher fluorescence signal. Styramide™ conjugates also allow significantly less consumption of primary antibody compared to standard directly conjugate method or tyramide amplification with the same level of sensitivity. iFluor® 594 PSA™ kit is a much superior replacement for Alexa Fluor 594 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.
Fluorescence IHC of formaldehyde-fixed, paraffin-embedded using PSA<strong> &trade; </strong>&nbsp;and TSA amplified methods. Human lung adenocarcinoma positive tissue sections were stained with mouse anti-EpCam antibody and then followed by PSA&trade;&nbsp;method using iFluor 594&trade; PSA&trade; Imaging Kit with Goat Anti-Mouse IgG (Cat#45280) or TSA method using&nbsp; Alexa Fluor&reg; 594 tyramide&nbsp; respectively.&nbsp; Images showed that PSA&trade; super signal amplification can increase the sensitivity of fluorescence IHC over Alexa Fluor&reg; 594 TSA method. Cell nucleus were stained with Nuclear Blue&trade; DCS1 (Cat#17548).
Fluorescence IHC of formaldehyde-fixed, paraffin-embedded using PSA<strong> &trade; </strong>&nbsp;and TSA amplified methods. Human lung adenocarcinoma positive tissue sections were stained with mouse anti-EpCam antibody and then followed by PSA&trade;&nbsp;method using iFluor 594&trade; PSA&trade; Imaging Kit with Goat Anti-Mouse IgG (Cat#45280) or TSA method using&nbsp; Alexa Fluor&reg; 594 tyramide&nbsp; respectively.&nbsp; Images showed that PSA&trade; super signal amplification can increase the sensitivity of fluorescence IHC over Alexa Fluor&reg; 594 TSA method. Cell nucleus were stained with Nuclear Blue&trade; DCS1 (Cat#17548).
CatalogSize
Price
Quantity
45280100 Tests
Price
 
Spectral properties

Absorbance (nm)587
Correction factor (260 nm)0.05
Correction factor (280 nm)0.04
Extinction coefficient (cm -1 M -1)
200000
1
Excitation (nm)587
Emission (nm)603
Quantum yield
0.53
1
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings

Fluorescence microscope
ExcitationCy3/TRITC filter set
EmissionCy3/TRITC filter set
Recommended plateBlack wall/clear bottom
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Page updated on December 19, 2025