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iFluor® 594 PSA™ Imaging Kit with Goat Anti-Rabbit IgG
Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. In combination with our superior iFluor® dyes that have higher florescence intensity, increased photostability and enhanced water solubility, the iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA™ utilizes the catalytic activity of horseradish peroxidase (HRP) for covalent deposition of fluorophores in situ. PSA™ radicals have much higher reactivity than tyramide radicals, making the PSA™ system much faster, more robust and sensitive than the traditional TSA reagents. Compared to tyramide reagents, the Styramide™ conjugates have ability to label the target at higher efficiency and thus generate significantly higher fluorescence signal. Styramide™ conjugates also allow significantly less consumption of primary antibody compared to standard directly conjugate method or tyramide amplification with the same level of sensitivity. iFluor® 594 PSA™ kit is a much superior replacement for Alexa Fluor 594 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.
Fluorescence IHC in formaldehyde-fixed paraffin-embedded tissue. Human lung adenocarcinoma sections were incubated with Rabbit mAb EpCAM then stained a HRP-labeled Goat anti-Rabbit IgG secondary antibody followed by iFluor® 594 Styramide™ (Left) or Alexa Fluor® 594 tyramide (Right), respectively. Fluorescence images were taken using the TRITC filter set and under the same exposure time. Cell nucleus was stained with Nuclear Blue™ DCS1 (Cat#17548).
Fluorescence IHC in formaldehyde-fixed paraffin-embedded tissue. Human lung adenocarcinoma sections were incubated with Rabbit mAb EpCAM then stained a HRP-labeled Goat anti-Rabbit IgG secondary antibody followed by iFluor® 594 Styramide™ (Left) or Alexa Fluor® 594 tyramide (Right), respectively. Fluorescence images were taken using the TRITC filter set and under the same exposure time. Cell nucleus was stained with Nuclear Blue™ DCS1 (Cat#17548).
protocol
Protocol
sds
SDS
CatalogSize
Price
Quantity
45230100 Tests
Price
 
Spectral properties
Absorbance (nm)587
Correction factor (260 nm)0.05
Correction factor (280 nm)0.04
Extinction coefficient (cm -1 M -1)
200000
1
Excitation (nm)587
Emission (nm)603
Quantum yield
0.53
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microscope
ExcitationCy3/TRITC filter set
EmissionCy3/TRITC filter set
Recommended plateBlack wall/clear bottom
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Power Styramide™ Signal Amplification (PSA™)
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Page updated on December 18, 2025