Wheat Germ Agglutinin (WGA)

iFluor® 594-Wheat Germ Agglutinin (WGA) Conjugate

Wheat germ agglutinin (WGA) is a lectin that binds to N-acetyl-D-glucosamine and sialic acid. It is of the most studied and useful lectins for it biological applications. Since WGA binds to glycoconjugates its derivatives and conjugates are widely used to label cell membranes and fibrotic scar tissue for fluorescence imaging and analysis. The carbohydrate-binding specificity of WGA is directed against sequences of β-1,4-GlcNAc-linked residues, the chitodextrins. Each monomer contains two identical, non-interacting binding sites which are complementary to 3 or 4 β-1,4-GlcNAc units. Of the monosaccharides examined, only GlcNAc binds to WGA. ManNAc does not bind and GalNAc binds only weakly. iFluor®594 conjugate of WGA might be the brightest red fluorescent WGA conjugate. It exhibits the bright, red fluorescence of the iFluor® 594 dye. iFluor®594 WGA conjugate binds to sialic acid and N-acetylglucosaminyl residues as AF594 WGA conjugate does.

Catalog	Size	Price	Quantity
25550	1 mg	Price

References

View all 50 references: Citation Explorer

Wheat germ agglutinin is involved in the protective action of 24-epibrassinolide on the roots of wheat seedlings under drought conditions.

Authors:

Avalbaev, Azamat and Bezrukova, Marina and Allagulova, Chulpan and Lubyanova, Alsu and Kudoyarova, Guzel and Fedorova, Kristina and Maslennikova, Dilara and Yuldashev, Ruslan and Shakirova, Farida

Journal:

Plant physiology and biochemistry : PPB (2020): 420-427

Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men.

Authors:

Janković, Tamara and Goč, Sanja and Mitić, Ninoslav and Danilović Luković, Jelena and Janković, Miroslava

Journal:

Upsala journal of medical sciences (2020): 10-18

Succinylated Wheat Germ Agglutinin Colocalizes with the Toxoplasma gondii Cyst Wall Glycoprotein CST1.

Authors:

Guevara, Rebekah B and Fox, Barbara A and Bzik, David J

Journal:

mSphere (2020)

Wheat germ agglutinin is a biomarker of whole grain content in wheat flour and pasta.

Authors:

Killilea, David W and McQueen, Rebecca and Abegania, Judi R

Journal:

Journal of food science (2020): 808-815

Wheat germ agglutinin liposomes with surface grafted cyclodextrins as bioadhesive dual-drug delivery nanocarriers to treat oral cells.

Authors:

Wijetunge, Sashini S and Wen, Jianchuan and Yeh, Chih-Ko and Sun, Yuyu

Journal:

Colloids and surfaces. B, Biointerfaces (2020): 110572

Physical properties

Solvent

Water

Spectral properties

Absorbance (nm)	587
Correction factor (260 nm)	0.05
Correction factor (280 nm)	0.04
Extinction coefficient (cm ^-1 M ^-1)	200000 ¹
Excitation (nm)	587
Emission (nm)	603
Quantum yield	0.53 ¹

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Instrument settings

Fluorescence microscope
Excitation	Cy3/TRITC filter set
Emission	Cy3/TRITC filter set
Recommended plate	Black wall/clear bottom

Documents

Protocol
Safety Data Sheet
Certificate of Origin
Certificate of Analysis

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Page updated on October 8, 2024