Name: iFluor®647-PEG12-dUTP *1 mM in TE Buffer (pH 7.5)*
Brand: AAT Bioquest
SKU: 17043
Price: 107 USD
Availability: InStock

iFluor®647-PEG12-dUTP

1 mM in TE Buffer (pH 7.5)

iFluor® 647-PEG12-dUTP is a far-red fluorescent, PEG12-linked nucleotide designed for enzymatic incorporation into DNA for fluorescence-based detection and imaging applications.

Enzymatic Labeling: Supports DNA labeling through PCR, nick translation, random priming, and reverse transcription.
PEG12 Spacer: Enhances incorporation efficiency by DNA polymerases.
Spectral Properties: Excitation/emission maxima of 655/670 nm.
High Fluorescence: Bright, photostable fluorescence with a quantum yield of 0.25.
Applications: Suitable for FISH probe preparation and DNA microarray analysis.

Fluorescence In Situ Hybridization of Cy5 and iFluor® 647-dUTP labelled Telomere probes in metaphase HeLa cells.

Datasheet

SDS

Catalog	Size	Price	Quantity
17043	25 nmoles	Price

Overview

The dye-modified deoxyuridine 5'-triphosphates are one of the most common methods to produce dye-labeled DNA via conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This iFluor® 647-PEG12-dUTP conjugate has the same spectral properties as ChromaTide™ Alexa Fluor™ 647-dUTP. Its PEG12 spacer makes it much more effectively incorporated into DNAs by polymerase. It is used as a far-red fluorescence color with a SpectrumFRed™ filter set. It provides a bright signal that has high photostability and is not affected by pH. ChromaTide™ and Alexa Fluor™ are the trademarks of ThermoFisher, and SpectrumFRed™ is the trademark of Vysis.

References

View all 38 references: Citation Explorer

Loss of PDPK1 abrogates resistance to gemcitabine in label-retaining pancreatic cancer cells.

Authors:

Li, Dandan and Mullinax, John E and Aiken, Taylor and Xin, Hongwu and Wiegand, Gordon and Anderson, Andrew and Thorgeirsson, Snorri and Avital, Itzhak and Rudloff, Udo

Journal:

BMC cancer (2018): 772

dUTPs conjugated with zwitterionic Cy3 or Cy5 fluorophore analogues are effective substrates for DNA amplification and labelling by Taq polymerase.

Authors:

Zasedateleva, Olga A and Vasiliskov, Vadim A and Surzhikov, Sergey A and Kuznetsova, Viktoriya E and Shershov, Valeriy E and Guseinov, Timur O and Smirnov, Igor P and Yurasov, Roman A and Spitsyn, Maksim A and Chudinov, Alexander V

Journal:

Nucleic acids research (2018): e73

Mass-spectrometry analysis of modifications at DNA termini induced by DNA polymerases.

Authors:

Smirnov, Igor P and Kolganova, Natalia A and Vasiliskov, Vadim A and Chudinov, Alexander V and Timofeev, Edward N

Journal:

Scientific reports (2017): 6674

Separation of replication and transcription domains in nucleoli.

Authors:

Smirnov, E and Borkovec, J and Kováčik, L and Svidenská, S and Schröfel, A and Skalníková, M and Švindrych, Z and Křížek, P and Ovesný, M and Hagen, G M and Juda, P and Michalová, K and Cardoso, M C and Cmarko, D and Raška, I

Journal:

Journal of structural biology (2014): 259-66

A prenatally ascertained, maternally inherited 14.8 Mb duplication of chromosomal bands Xq13.2-q21.31 associated with multiple congenital abnormalities in a male fetus.

Authors:

Sismani, C and Donoghue, J and Alexandrou, A and Karkaletsi, M and Christopoulou, S and Konstantinidou, A E and Livanos, P and Patsalis, P C and Velissariou, V

Journal:

Gene (2013): 138-42

Physical properties

Molecular weight	2293.72
Solvent	Water

Spectral properties

Correction factor (260 nm)	0.03
Correction factor (280 nm)	0.03
Correction factor (656 nm)	0.0793
Extinction coefficient (cm ^-1 M ^-1)	220000 ¹
Excitation (nm)	655
Emission (nm)	670
Quantum yield	0.25 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 31, 2026