Wheat Germ Agglutinin (WGA)

iFluor® 647-Wheat Germ Agglutinin (WGA) Conjugate

iFluor® 647-Wheat Germ Agglutinin (WGA) Conjugate is a far-red fluorescent lectin probe designed for detecting and visualizing cell-surface glycoconjugates in live and fixed cells.

Binding Specificity: Binds N-acetyl-D-glucosamine and sialic acid residues.
Spectral Properties: Excitation/emission maxima of 655/670 nm.
Filter Compatibility: Compatible with Cy5 fluorescence microscope filter sets.
Applications: Suitable for cell membrane and fibrotic scar tissue staining.

Live HeLa cells were stained with iFluor® 647-Wheat Germ Agglutinin (WGA) Conjugate at 5 µg/mL for 30 minutes followed by Hoechst 33342 (AAT Cat# 17535). Image was acquired using fluorescence microscopy using Cy5 and DAPI filter set.

Catalog	Size	Price	Quantity
25559	1 mg	Price

Overview

Wheat germ agglutinin (WGA) is a lectin that binds to N-acetyl-D-glucosamine and sialic acid. It is of the most studied and useful lectins for it biological applications. Since WGA binds to glycoconjugates its derivatives and conjugates are widely used to label cell membranes and fibrotic scar tissue for fluorescence imaging and analysis. The carbohydrate-binding specificity of WGA is directed against sequences of β-1,4-GlcNAc-linked residues, the chitodextrins. Each monomer contains two identical, non-interacting binding sites which are complementary to 3 or 4 β-1,4-GlcNAc units. Of the monosaccharides examined, only GlcNAc binds to WGA. ManNAc does not bind and GalNAc binds only weakly. iFluor®647 conjugate of WGA exhibits the bright, red fluorescence of the iFluor® 647 dye. iFluor®647 WGA conjugate binds to sialic acid and N-acetylglucosaminyl residues as AF647 WGA conjugate does.

Citations

View all 11 citations: Citation Explorer

Dynamic Fluid Flow and Endothelial Crosstalk Regulate Mesangial Homeostasis in a Simplified 3D Co-culture Model

Authors:

Yoshihara, Maki and Nishiyama, Megumi and Matsubara, Ayano and Fukumoto, Hina and Narita, Takayuki and Kawasaki, Maki and Mine, Yumeka and Sakumoto, Takehisa and Iwamoto, Shuhei and Fukuda, Makoto and others,

Journal:

American Journal of Physiology-Renal Physiology (2026)

A probiotic--drug cascade system for inflammatory bowel disease via tryptophan metabolism and gut microbiome remodeling

Authors:

Yang, Junfei and Feng, Zuer and Sun, Shuxin and Pei, Tianqi and Li, Yunfei and Xue, Beiyang and Zhou, Chuangxi and Song, Qingling and Zhang, Yun and Wang, Lei

Journal:

Acta Pharmaceutica Sinica B (2026)

Integrin alpha8beta1 is Identified as a Potential CAR-T Target for BCMA-Resistant Relapsed Multiple Myeloma

Authors:

Liu, Suiping and Wu, Jieying and Liu, Jiayu and Wu, Kangxiu and Huang, Yanyan and Geng, Suxia and Wang, Yulian and Weng, Jianyu and Du, Xin and Lai, Peilong

Journal:

ImmunoTargets and Therapy (2026): 605729

Sesn2 is Associated with Attenuated Muscle Atrophy and Altered Expression of Key Myogenic and Autophagy Markers in Mdx Mice

Authors:

Song, Zubiao and Lin, Qing and Liang, Jiahui and He, Juanjuan and Zhang, Weixi

Journal:

Journal of Molecular Neuroscience (2026): 64

Localized Delivery of Hyaluronic Acid-Doxorubicin from a Surgical Paste for Post-operative Glioblastoma Treatment

Authors:

Rodella, Giulia and Pesce, Cristiano and Rampado, Riccardo and Garofalo, Mariangela and Wang, Mingchao and Ucakar, Bernard and Vanvarenberg, Kevin and Ma, Zhanjun and Joudiou, Nicolas and Pr{\'e}at, V{\'e}ronique and others,

Journal:

Materials Today Bio (2026): 103142

References

View all 49 references: Citation Explorer

Wheat germ agglutinin is a biomarker of whole grain content in wheat flour and pasta.

Authors:

Killilea, David W and McQueen, Rebecca and Abegania, Judi R

Journal:

Journal of food science (2020): 808-815

Wheat germ agglutinin-conjugated fluorescent pH sensors for visualizing proton fluxes.

Authors:

Zhang, Lejie and Zhang, Mei and Bellve, Karl and Fogarty, Kevin E and Castro, Maite A and Brauchi, Sebastian and Kobertz, William R

Journal:

The Journal of general physiology (2020)

Wheat germ agglutinin liposomes with surface grafted cyclodextrins as bioadhesive dual-drug delivery nanocarriers to treat oral cells.

Authors:

Wijetunge, Sashini S and Wen, Jianchuan and Yeh, Chih-Ko and Sun, Yuyu

Journal:

Colloids and surfaces. B, Biointerfaces (2020): 110572

Succinylated Wheat Germ Agglutinin Colocalizes with the Toxoplasma gondii Cyst Wall Glycoprotein CST1.

Authors:

Guevara, Rebekah B and Fox, Barbara A and Bzik, David J

Journal:

mSphere (2020)

Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men.

Authors:

Janković, Tamara and Goč, Sanja and Mitić, Ninoslav and Danilović Luković, Jelena and Janković, Miroslava

Journal:

Upsala journal of medical sciences (2020): 10-18

Physical properties

Solvent

Water

Spectral properties

Correction factor (260 nm)	0.03
Correction factor (280 nm)	0.03
Correction factor (656 nm)	0.0793
Extinction coefficient (cm ^-1 M ^-1)	220000 ¹
Excitation (nm)	655
Emission (nm)	670
Quantum yield	0.25 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Fluorescence microscope
Excitation	Cy5 filter set
Emission	Cy5 filter set
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 2, 2026