Name: iFluor® 660 Styramide
Brand: AAT Bioquest
SKU: 44906
Price: 329 USD
Availability: InStock

iFluor® 660 Styramide

Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA™ utilizes the catalytic activity of horseradish peroxidase (HRP) for covalent deposition of fluorophores in situ. PSA™ radicals have much higher reactivity than tyramide radicals, making the PSA™ system much faster, more robust and sensitive than the traditional TSA reagents. iFluor® 660 Styramide is a new unique far red fluorescent PSA™ reagent for multicolor application with our existing PSA™ and TSA reagents. Its maximum fluorescence falls between Cy5/Alexa Fluor® 647 and Cy5.5/ Alexa Fluor® 647. AAT Bioquest offers the largest collection of TSA regents. We are the exclusive source of the superior PSA™ reagents for multicolor applications.

Fluorescence IHC of formaldehyde-fixed, paraffin-embedded human lung adenocarcinoma positive tissue using PSA<strong> ™</strong> amplified methods. Human lung adenocarcinoma positive tissue sections were stained with Mouse anti-EpCAM or Control Mouse IgG antibody and then incubated with polyHRP-labeled Goat anti-Mouse IgG secondary antibody followed by iFluor® 660 Styramide™ (Cat#44906).

Protocol

SDS

Catalog	Size	Price	Quantity
44906	100 Slides	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 660 Styramide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	68.356 µL	341.778 µL	683.555 µL	3.418 mL	6.836 mL
5 mM	13.671 µL	68.356 µL	136.711 µL	683.555 µL	1.367 mL
10 mM	6.836 µL	34.178 µL	68.356 µL	341.778 µL	683.555 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1462.94 g/mol	=	mL	x	mM	=	mmol

References

View all 4 references: Citation Explorer

Quantum dot-based FRET for sensitive determination of hydrogen peroxide and glucose using tyramide reaction.

Authors:

Huang, Xiangyi and Wang, Jinjie and Liu, Heng and Lan, Tao and Ren, Jicun

Journal:

Talanta (2013): 79-84

Dual enhancement of triple immunofluorescence using two antibodies from the same species.

Authors:

Nakamura, Ayako and Uchihara, Toshiki

Journal:

Journal of neuroscience methods (2004): 67-70

Rapid detection and enumeration of Naegleria fowleri in surface waters by solid-phase cytometry.

Authors:

Pougnard, Claire and Catala, Philippe and Drocourt, Jean-Louis and Legastelois, Stephane and Pernin, Pierre and Pringuez, Emmanuelle and Lebaron, Philippe

Journal:

Applied and environmental microbiology (2002): 3102-7

Oligonucleotides as hybridization probes to localize phytoplasmas in host plants and insect vectors.

Authors:

Webb, D R and Bonfiglioli, R G and Carraro, L and Osler, R and Symons, R H

Journal:

Phytopathology (1999): 894-901

Physical properties

Molecular weight	1462.94
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.07
Correction factor (280 nm)	0.08
Extinction coefficient (cm ^-1 M ^-1)	250000 ¹
Excitation (nm)	663
Emission (nm)	678
Quantum yield	0.26 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Instrument settings

Fluorescence microscope
Excitation	Cy5 filter set
Emission	Cy5 filter set
Recommended plate	Black wall/clear bottom

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Page updated on January 25, 2026