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iFluor® 660 Styramide
Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA utilizes the catalytic activity of horseradish peroxidase (HRP) for covalent deposition of fluorophores in situ. PSA radicals have much higher reactivity than tyramide radicals, making the PSA system much faster, more robust and sensitive than the traditional TSA reagents. iFluor® 660 Styramide is a new unique far red fluorescent PSA reagent for multicolor application with our existing PSA and TSA reagents. Its maximum fluorescence falls between Cy5/Alexa Fluor® 647 and Cy5.5/ Alexa Fluor® 647. AAT Bioquest offers the largest collection of TSA regents. We are the exclusive source of the superior PSA reagents for multicolor applications.
Fluorescence IHC of formaldehyde-fixed, paraffin-embedded human lung adenocarcinoma positive tissue using PSA<strong>&nbsp;&trade;</strong>&nbsp;amplified methods. Human lung adenocarcinoma positive tissue sections were stained with&nbsp;Mouse anti-EpCAM or Control Mouse IgG antibody and then incubated with polyHRP-labeled Goat anti-Mouse IgG secondary antibody followed by iFluor® 660 Styramide&trade; (Cat#44906).
Fluorescence IHC of formaldehyde-fixed, paraffin-embedded human lung adenocarcinoma positive tissue using PSA<strong>&nbsp;&trade;</strong>&nbsp;amplified methods. Human lung adenocarcinoma positive tissue sections were stained with&nbsp;Mouse anti-EpCAM or Control Mouse IgG antibody and then incubated with polyHRP-labeled Goat anti-Mouse IgG secondary antibody followed by iFluor® 660 Styramide&trade; (Cat#44906).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
44906100 Slides
Price
 
Physical properties
Molecular weight1462.94
SolventDMSO
Spectral properties
Correction factor (260 nm)0.07
Correction factor (280 nm)0.08
Extinction coefficient (cm -1 M -1)
250000
1
Excitation (nm)663
Emission (nm)678
Quantum yield
0.26
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Instrument settings
Fluorescence microscope
ExcitationCy5 filter set
EmissionCy5 filter set
Recommended plateBlack wall/clear bottom
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Page updated on October 13, 2025