iFluor® 680-streptavidin conjugate

iFluor® 680-streptavidin conjugate is a far-red fluorescent probe designed for detecting biotinylated proteins, antibodies, nucleic acids, and other biomolecules through high-affinity streptavidin-biotin binding.

Binding Specificity: Exhibits high-affinity binding to biotinylated targets.
Spectral Properties: Excitation/emission maxima of 684/701 nm.
High Fluorescence: Features bright, photostable iFluor® 680 dye with minimal protein quenching.
Dye Compatibility: Spectrally similar to Alexa Fluor® 680-streptavidin conjugate.

<p>HeLa cells were incubated with mouse anti-tubulin and biotin goat anti-mouse IgG followed by AAT’s iFluor® 680-streptavidin conjugate (Red). Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17530).</p>

Datasheet

Catalog	Size	Price	Quantity
16968	200 ug	Price
16997	1 mg	Price

Overview

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. A variety of the complementary biotinylated reagents are available from numerous commercial vendors.This iFluor® 680-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with iFluor® 680 covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. iFluor® 680-streptavidin conjugates has fluorescence excitation and emission maxima of ~682 nm and ~701 nm respectively. These spectral characteristics make it an excellent alternative to Alexa Fluor® 680-streptavidin conjugate (Alexa Fluor® is the trademark of Invitrogen). It is a very valuable tool for biotin-streptavidin-based biological assays and tests.

References

View all 47 references: Citation Explorer

A streptavidin paramagnetic-particle based competition assay for the evaluation of the optical selectivity of quadruplex nucleic acid fluorescent probes

Authors:

Largy E, Hamon F, Teulade-Fichou MP.

Journal:

Methods. (2012)

Biotin-4-fluorescein based fluorescence quenching assay for determination of biotin binding capacity of streptavidin conjugated quantum dots

Authors:

Mittal R, Bruchez MP.

Journal:

Bioconjug Chem (2011): 362

Iminobiotin binding induces large fluorescent enhancements in avidin and streptavidin fluorescent conjugates and exhibits diverging pH-dependent binding affinities

Authors:

Raphael MP, Rappole CA, Kurihara LK, Christodoulides JA, Qadri SN, Byers JM.

Journal:

J Fluoresc (2011): 647

Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex

Authors:

Kim JH, Chang TM, Graham AN, Choo KH, Kalitsis P, Hudson DF.

Journal:

BMC Biochem (2010): 50

Determination of 17beta-oestradiol by fluorescence immunoassay with streptavidin-conjugated quantum dots as label

Authors:

Sun M, Du L, Gao S, Bao Y, Wang S.

Journal:

Steroids (2010): 400

Physical properties

Molecular weight	~52000
Solvent	Water

Spectral properties

Correction factor (260 nm)	0.097
Correction factor (280 nm)	0.094
Extinction coefficient (cm ^-1 M ^-1)	220000 ¹
Excitation (nm)	684
Emission (nm)	701
Quantum yield	0.23 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 28, 2026