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iFluor® 750-streptavidin conjugate
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. A variety of the complementary biotinylated reagents are available from numerous commercial vendors.This iFluor® 750-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with iFluor® 750 covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. iFluor® 750-streptavidin conjugates has fluorescence excitation and emission maxima of ~753 nm and ~779 nm respectively. These spectral characteristics make it an excellent alternative to Alexa Fluor® 750-streptavidin conjugate (Alexa Fluor® is the trademark of Invitrogen). It is a very valuable tool for biotin-streptavidin-based biological assays and tests.
<p>HL-60 cells were incubated with (Red, +) or without (Green, -) mouse Anti-Human HLA-ABC Biotin (W6/32-Biotin) followed by iFluor® 750-streptavidin conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in APC-C7 channel.</p>
<p>HL-60 cells were incubated with (Red, +) or without (Green, -) mouse Anti-Human HLA-ABC Biotin (W6/32-Biotin) followed by iFluor® 750-streptavidin conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in APC-C7 channel.</p>
protocol
Protocol
CatalogSize
Price
Quantity
16973200 ug
Price
169991 mg
Price
 
Physical properties
Molecular weight~52000
SolventWater
Spectral properties
Correction factor (260 nm)0.044
Correction factor (280 nm)0.039
Correction factor (565 nm)0.0250
Correction factor (650 nm)0.1413
Extinction coefficient (cm -1 M -1)
275000
1
Excitation (nm)757
Emission (nm)779
Quantum yield
0.12
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Documents
Protocol
Safety Data Sheet (Catalog 16973)
Safety Data Sheet (Catalog 16999)
Certificate of Analysis
Brochure: Secondary Detection Probes & Kits
Brochure: Fluorescent Infrared Dyes & Probes
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Page updated on October 30, 2025