iFluor® 790-streptavidin conjugate
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. A variety of the complementary biotinylated reagents are available from numerous commercial vendors. This iFluor® 790-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with iFluor® 790 covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. iFluor® 790-streptavidin conjugates has fluorescence excitation and emission maxima of ~786 nm and ~811 nm respectively. These spectral characteristics make it an excellent alternative to Alexa Fluor® 790-streptavidin conjugate (Alexa Fluor® is the trademark of Invitrogen). It is a very valuable tool for biotin-streptavidin-based biological assays and tests. iFluor® 790 dye emission is well separated from commonly used far-red fluorophores such as Cy5, Cy7 or allophycocyanin (APC), facilitating multicolor analysis. This fluorophore is also useful for small animal in-vivo imaging applications or for other imaging applications that require NIR detections such as the two-color western applications with the LI-COR® Odyssey® infrared imaging system.
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 350-streptavidin conjugate | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
iFluor® 405-streptavidin conjugate | 403 | 427 | 370001 | 0.911 | 0.48 | 0.77 |
iFluor® 488-streptavidin conjugate | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 514-streptavidin conjugate | 511 | 527 | 750001 | 0.831 | 0.265 | 0.116 |
iFluor® 532-streptavidin conjugate | 537 | 560 | 900001 | 0.681 | 0.26 | 0.16 |
iFluor® 555-streptavidin conjugate | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 594-streptavidin conjugate | 587 | 603 | 2000001 | 0.531 | 0.05 | 0.04 |
iFluor® 633-streptavidin conjugate | 640 | 654 | 2500001 | 0.291 | 0.062 | 0.044 |
iFluor® 647-streptavidin conjugate | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
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Citations
View all 16 citations: Citation Explorer
Aptamer Structure Switch Fluorescence Anisotropy Assay for Small Molecules Using Streptavidin as an Effective Signal Amplifier Based on Proximity Effect
Authors: Li, Y., Zhao, Q.
Journal: Anal Chem (2019): 7379-7384
Authors: Li, Y., Zhao, Q.
Journal: Anal Chem (2019): 7379-7384
Streptavidin cooperative allosterism upon binding biotin observed by differential changes in intrinsic fluorescence
Authors: Waner, M. J., Hiznay, J. M., Mustovich, A. T., Patton, W., Ponyik, C., Mascotti, D. P.
Journal: Biochem Biophys Rep (2019): 127-131
Authors: Waner, M. J., Hiznay, J. M., Mustovich, A. T., Patton, W., Ponyik, C., Mascotti, D. P.
Journal: Biochem Biophys Rep (2019): 127-131
Streptavidin interfacing as a general strategy to localize fluorescent membrane tension probes in cells
Authors: Goujon, A., Strakova, K., Sakai, N., Matile, S.
Journal: Chem Sci (2019): 310-319
Authors: Goujon, A., Strakova, K., Sakai, N., Matile, S.
Journal: Chem Sci (2019): 310-319
Streptavidin-conjugated gold nanoclusters as ultrasensitive fluorescent sensors for early diagnosis of HIV infection
Authors: Kurdekar, A. D., Avinash Chunduri, L. A., Manohar, C. S., Haleyurgirisetty, M. K., Hewlett, I. K., Venkataramaniah, K.
Journal: Sci Adv (2018): eaar6280
Authors: Kurdekar, A. D., Avinash Chunduri, L. A., Manohar, C. S., Haleyurgirisetty, M. K., Hewlett, I. K., Venkataramaniah, K.
Journal: Sci Adv (2018): eaar6280
Design and Fabrication of Streptavidin-Functionalized, Fluorescently Labeled Polymeric Nanocarriers
Authors: Jo, A., Zhang, R., Allen, I. C., Riffle, J. S., Davis, R. M.
Journal: Langmuir (2018): 15783-15794
Authors: Jo, A., Zhang, R., Allen, I. C., Riffle, J. S., Davis, R. M.
Journal: Langmuir (2018): 15783-15794
References
View all 1 references: Citation Explorer
Aluminum phthalocyanine-streptavidin: new, sensitive fluorescent tracer for immunoassay
Authors: Kelly TA, Hunter CA, Schindele DC, Pepich BV.
Journal: Clin Chem (1991): 1283
Authors: Kelly TA, Hunter CA, Schindele DC, Pepich BV.
Journal: Clin Chem (1991): 1283
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