Name: iFluor® 790-streptavidin conjugate
Brand: AAT Bioquest
SKU: 16975
Price: 118 USD
Availability: InStock

iFluor® 790-streptavidin conjugate

iFluor® 790-streptavidin conjugate is a near-infrared fluorescent probe designed for detecting biotinylated proteins, antibodies, nucleic acids, and other biomolecules through high-affinity streptavidin-biotin binding.

Binding Specificity: Exhibits high-affinity binding to biotinylated targets.
Spectral Properties: Excitation/emission maxima of 787/812 nm.
Spectral Compatibility: Emission is well separated from Cy5, Cy7, and APC fluorophores.
Applications: Suitable for indirect immunofluorescence, near-infrared animal imaging, and two-color Western blot detection.

AAT Bioquest's bright and photostable iFluor® dyes cover the entire spectrum of UV, Vis and IR from 200 to 900 nm. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, 633, 647 and 860 nm). iFluor® dye-labeled streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin.

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
16975	200 ug	Price

Overview

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. A variety of the complementary biotinylated reagents are available from numerous commercial vendors. This iFluor® 790-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with iFluor® 790 covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. iFluor® 790-streptavidin conjugates has fluorescence excitation and emission maxima of ~786 nm and ~811 nm respectively. These spectral characteristics make it an excellent alternative to Alexa Fluor® 790-streptavidin conjugate (Alexa Fluor® is the trademark of Invitrogen). It is a very valuable tool for biotin-streptavidin-based biological assays and tests. iFluor® 790 dye emission is well separated from commonly used far-red fluorophores such as Cy5, Cy7 or allophycocyanin (APC), facilitating multicolor analysis. This fluorophore is also useful for small animal in-vivo imaging applications or for other imaging applications that require NIR detections such as the two-color western applications with the LI-COR® Odyssey® infrared imaging system.

Citations

View all 16 citations: Citation Explorer

Aptamer Structure Switch Fluorescence Anisotropy Assay for Small Molecules Using Streptavidin as an Effective Signal Amplifier Based on Proximity Effect

Authors:

Li, Y., Zhao, Q.

Journal:

Anal Chem (2019): 7379-7384

Streptavidin cooperative allosterism upon binding biotin observed by differential changes in intrinsic fluorescence

Authors:

Waner, M. J., Hiznay, J. M., Mustovich, A. T., Patton, W., Ponyik, C., Mascotti, D. P.

Journal:

Biochem Biophys Rep (2019): 127-131

Streptavidin interfacing as a general strategy to localize fluorescent membrane tension probes in cells

Authors:

Goujon, A., Strakova, K., Sakai, N., Matile, S.

Journal:

Chem Sci (2019): 310-319

Streptavidin-conjugated gold nanoclusters as ultrasensitive fluorescent sensors for early diagnosis of HIV infection

Authors:

Kurdekar, A. D., Avinash Chunduri, L. A., Manohar, C. S., Haleyurgirisetty, M. K., Hewlett, I. K., Venkataramaniah, K.

Journal:

Sci Adv (2018): eaar6280

Design and Fabrication of Streptavidin-Functionalized, Fluorescently Labeled Polymeric Nanocarriers

Authors:

Jo, A., Zhang, R., Allen, I. C., Riffle, J. S., Davis, R. M.

Journal:

Langmuir (2018): 15783-15794

Physical properties

Molecular weight	~52000
Solvent	Water

Spectral properties

Correction factor (260 nm)	0.1
Correction factor (280 nm)	0.09
Extinction coefficient (cm ^-1 M ^-1)	250000 ¹
Excitation (nm)	787
Emission (nm)	812
Quantum yield	0.13 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on November 6, 2026