iFluor® 800-streptavidin conjugate
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | ~52000 |
Solvent | Water |
Spectral properties
Correction Factor (260 nm) | 0.03 |
Correction Factor (280 nm) | 0.08 |
Extinction coefficient (cm -1 M -1) | 2500001 |
Excitation (nm) | 801 |
Emission (nm) | 820 |
Quantum yield | 0.111 |
Storage, safety and handling
Intended use | Research Use Only (RUO) |
Storage | Freeze (< -15 °C); Minimize light exposure |
Overview | ![]() ![]() |
See also: Biotin and Streptavidin
Molecular weight ~52000 | Correction Factor (260 nm) 0.03 | Correction Factor (280 nm) 0.08 | Extinction coefficient (cm -1 M -1) 2500001 | Excitation (nm) 801 | Emission (nm) 820 | Quantum yield 0.111 |
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. A variety of the complementary biotinylated reagents are available from numerous commercial vendors. This iFluor® 800-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with iFluor® 800 covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. iFluor® 800-streptavidin conjugates has fluorescence excitation and emission maxima of ~800 nm and ~820 nm respectively. These spectral characteristics make it an excellent alternative to IRDye® 800CW (IRDye® 800CW is the trademark of Li-COR) due to its much longer excitation wavelength (making it much more tissue-penetrating than IRDye® 800CW). It is a very valuable tool for biotin-streptavidin-based biological assays and tests.
Spectrum
Open in Advanced Spectrum Viewer


Spectral properties
Correction Factor (260 nm) | 0.03 |
Correction Factor (280 nm) | 0.08 |
Extinction coefficient (cm -1 M -1) | 2500001 |
Excitation (nm) | 801 |
Emission (nm) | 820 |
Quantum yield | 0.111 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 350-streptavidin conjugate | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
iFluor® 405-streptavidin conjugate | 403 | 427 | 370001 | 0.911 | 0.48 | 0.77 |
iFluor® 488-streptavidin conjugate | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 514-streptavidin conjugate | 511 | 527 | 750001 | 0.831 | 0.265 | 0.116 |
iFluor® 532-streptavidin conjugate | 537 | 560 | 900001 | 0.681 | 0.26 | 0.16 |
iFluor® 555-streptavidin conjugate | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 594-streptavidin conjugate | 588 | 604 | 1800001 | 0.531 | 0.05 | 0.04 |
iFluor® 633-streptavidin conjugate | 640 | 654 | 2500001 | 0.291 | 0.062 | 0.044 |
iFluor® 647-streptavidin conjugate | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
Show More (9) |
Images

Figure 1. AAT Bioquest's bright and photostable iFluor® dyes cover the entire spectrum of UV, Vis and IR from 200 to 900 nm. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, 633, 647 and 860 nm). iFluor® dye-labeled streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin.
Citations
View all 16 citations: Citation Explorer
Primary and Recall Immune Responses to SARS-CoV-2 in Breakthrough Infection
Authors: D’Orso, Silvia and Pirronello, Marta and Verdiani, Alice and Rossini, Angelo and Guerrera, Gisella and Picozza, Mario and Sambucci, Manolo and Misiti, Andrea and De Marco, Lorenzo and Salvia, Antonino and others,
Journal: Vaccines (2023): 1705
Authors: D’Orso, Silvia and Pirronello, Marta and Verdiani, Alice and Rossini, Angelo and Guerrera, Gisella and Picozza, Mario and Sambucci, Manolo and Misiti, Andrea and De Marco, Lorenzo and Salvia, Antonino and others,
Journal: Vaccines (2023): 1705
Aptamer Structure Switch Fluorescence Anisotropy Assay for Small Molecules Using Streptavidin as an Effective Signal Amplifier Based on Proximity Effect
Authors: Li, Y., Zhao, Q.
Journal: Anal Chem (2019): 7379-7384
Authors: Li, Y., Zhao, Q.
Journal: Anal Chem (2019): 7379-7384
Streptavidin cooperative allosterism upon binding biotin observed by differential changes in intrinsic fluorescence
Authors: Waner, M. J., Hiznay, J. M., Mustovich, A. T., Patton, W., Ponyik, C., Mascotti, D. P.
Journal: Biochem Biophys Rep (2019): 127-131
Authors: Waner, M. J., Hiznay, J. M., Mustovich, A. T., Patton, W., Ponyik, C., Mascotti, D. P.
Journal: Biochem Biophys Rep (2019): 127-131
Streptavidin interfacing as a general strategy to localize fluorescent membrane tension probes in cells
Authors: Goujon, A., Strakova, K., Sakai, N., Matile, S.
Journal: Chem Sci (2019): 310-319
Authors: Goujon, A., Strakova, K., Sakai, N., Matile, S.
Journal: Chem Sci (2019): 310-319
Streptavidin-conjugated gold nanoclusters as ultrasensitive fluorescent sensors for early diagnosis of HIV infection
Authors: Kurdekar, A. D., Avinash Chunduri, L. A., Manohar, C. S., Haleyurgirisetty, M. K., Hewlett, I. K., Venkataramaniah, K.
Journal: Sci Adv (2018): eaar6280
Authors: Kurdekar, A. D., Avinash Chunduri, L. A., Manohar, C. S., Haleyurgirisetty, M. K., Hewlett, I. K., Venkataramaniah, K.
Journal: Sci Adv (2018): eaar6280
Design and Fabrication of Streptavidin-Functionalized, Fluorescently Labeled Polymeric Nanocarriers
Authors: Jo, A., Zhang, R., Allen, I. C., Riffle, J. S., Davis, R. M.
Journal: Langmuir (2018): 15783-15794
Authors: Jo, A., Zhang, R., Allen, I. C., Riffle, J. S., Davis, R. M.
Journal: Langmuir (2018): 15783-15794
Optimized Streptavidin for Fluorescent Labeling of Biotinylated Targets
Authors: Hytonen, V. P.
Journal: Cell Chem Biol (2017): 921-922
Authors: Hytonen, V. P.
Journal: Cell Chem Biol (2017): 921-922
Quantum dot-fluorescence in situ hybridisation for Ectromelia virus detection based on biotin-streptavidin interactions
Authors: Wang, T., Zheng, Z., Zhang, X. E., Wang, H.
Journal: Talanta (2016): 179-184
Authors: Wang, T., Zheng, Z., Zhang, X. E., Wang, H.
Journal: Talanta (2016): 179-184
Streptavidin-triggered signal amplified fluorescence polarization for analysis of DNA-protein interactions
Authors: Choi, J. W., Jo, B. G., deMello, A. J., Choo, J., Kim, H. Y.
Journal: Analyst (2016): 6499-6502
Authors: Choi, J. W., Jo, B. G., deMello, A. J., Choo, J., Kim, H. Y.
Journal: Analyst (2016): 6499-6502
Novel biosensor system model based on fluorescence quenching by a fluorescent streptavidin and carbazole-labeled biotin
Authors: Zhu, X., Shinohara, H., Miyatake, R., Hohsaka, T.
Journal: J Mol Recognit (2016): 485-91
Authors: Zhu, X., Shinohara, H., Miyatake, R., Hohsaka, T.
Journal: J Mol Recognit (2016): 485-91
References
View all 1 references: Citation Explorer
Aluminum phthalocyanine-streptavidin: new, sensitive fluorescent tracer for immunoassay
Authors: Kelly TA, Hunter CA, Schindele DC, Pepich BV.
Journal: Clin Chem (1991): 1283
Authors: Kelly TA, Hunter CA, Schindele DC, Pepich BV.
Journal: Clin Chem (1991): 1283