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iFluor® 860-streptavidin conjugate

AAT Bioquest's bright and photostable iFluor® dyes cover the entire spectrum of UV, Vis and IR from 200 to 900 nm. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, 633, 647 and 860 nm). iFluor® dye-labeled streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin.
AAT Bioquest's bright and photostable iFluor® dyes cover the entire spectrum of UV, Vis and IR from 200 to 900 nm. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, 633, 647 and 860 nm). iFluor® dye-labeled streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin.
Ordering information
Price ()
Catalog Number16979
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight52000
SolventWater
Spectral properties
Correction Factor (260 nm)0.1
Correction Factor (280 nm)0.14
Extinction coefficient (cm -1 M -1)2500001
Excitation (nm)853
Emission (nm)878
Storage, safety and handling
Intended useResearch Use Only (RUO)
StorageFreeze (< -15 °C); Minimize light exposure
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iFluor® 750 maleimide
iFluor® 350 amine
iFluor® 405 amine
iFluor® 488 amine
iFluor® 555 amine
iFluor® 647 amine
iFluor® 660 amine
iFluor® 680 amine
iFluor® 700 amine
iFluor® 710 amine
iFluor® 750 amine
iFluor® 350 hydrazide
iFluor® 488 hydrazide
iFluor® 555 hydrazide
iFluor® 647 hydrazide
iFluor® 680 hydrazide
iFluor® 700 hydrazide
iFluor® 750 hydrazide
iFluor® 647 alkyne
iFluor® 647 azide
iFluor® 790 acid
iFluor® 790 amine
iFluor® 790 hydrazide
iFluor® 790 maleimide
iFluor® 488 tyramide
iFluor® 350 goat anti-mouse IgG (H+L)
iFluor® 405 goat anti-mouse IgG (H+L)
iFluor® 488 goat anti-mouse IgG (H+L)
iFluor® 514 goat anti-mouse IgG (H+L)
iFluor® 532 goat anti-mouse IgG (H+L)
iFluor® 555 goat anti-mouse IgG (H+L)
iFluor® 594 goat anti-mouse IgG (H+L)
iFluor® 633 goat anti-mouse IgG (H+L)
iFluor® 647 goat anti-mouse IgG (H+L)
iFluor® 680 goat anti-mouse IgG (H+L)
iFluor® 700 goat anti-mouse IgG (H+L)
iFluor® 750 goat anti-mouse IgG (H+L)
iFluor® 790 goat anti-mouse IgG (H+L)
iFluor® 350 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 405 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 488 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 514 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 532 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 555 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 594 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 633 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 647 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 680 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 700 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 750 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 790 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 350 goat anti-rabbit IgG (H+L)
iFluor® 405 goat anti-rabbit IgG (H+L)
iFluor® 488 goat anti-rabbit IgG (H+L)
iFluor® 514 goat anti-rabbit IgG (H+L)
iFluor® 532 goat anti-rabbit IgG (H+L)
iFluor® 555 goat anti-rabbit IgG (H+L)
iFluor® 594 goat anti-rabbit IgG (H+L)
iFluor® 633 goat anti-rabbit IgG (H+L)
iFluor® 647 goat anti-rabbit IgG (H+L)
iFluor® 680 goat anti-rabbit IgG (H+L)
iFluor® 700 goat anti-rabbit IgG (H+L)
iFluor® 750 goat anti-rabbit IgG (H+L)
iFluor® 790 goat anti-rabbit IgG (H+L)
iFluor® 350 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 405 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 488 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 514 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 532 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 555 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 594 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 633 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 647 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 680 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 700 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 750 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 790 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor 750™ RGD Conjugate
iFluor® 488 azide
iFluor® 405 hydrazide
iFluor® 800 acid
iFluor® 800 maleimide
iFluor® 810 acid
iFluor® 810 maleimide
iFluor® 820 acid
iFluor® 820 maleimide
iFluor® 860 acid
iFluor® 860 maleimide
iFluor® 546 goat anti-mouse IgG (H+L)
iFluor® 546 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 568 goat anti-mouse IgG (H+L)
iFluor® 568 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 546 goat anti-rabbit IgG (H+L)
iFluor® 546 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 568 goat anti-rabbit IgG (H+L)
iFluor® 568 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 555 alkyne
iFluor® 555 azide
iFluor® 532 maleimide
iFluor® 594 maleimide
iFluor® 488 alkyne
iFluor® 405 maleimide
iFluor® 430 maleimide
iFluor® 568 maleimide
iFluor® 633 maleimide
iFluor® 450 maleimide
iFluor® 350 Styramide *Superior Replacement for Alexa Fluor 350 tyramide*
iFluor® 488 Styramide *Superior Replacement for Alexa Fluor 488 tyramide and Opal 520*
iFluor® 546 Styramide *Superior Replacement for Alexa Fluor 546 tyramide*
iFluor® 555 Styramide *Superior Replacement for Alexa Fluor 555 tyramide and Opal 570*
iFluor® 568 Styramide *Superior Replacement for Alexa Fluor 568 tyramide*
iFluor® 594 Styramide *Superior Replacement for Alexa Fluor 594 tyramide*
iFluor® 647 Styramide *Superior Replacement for Alexa Fluor 647 tyramide*
iFluor® 680 Styramide *Superior Replacement for Alexa Fluor 680 tyramide and Opal 690*
iFluor® 700 Styramide *Superior Replacement for Alexa Fluor 700 tyramide*
iFluor® 750 Styramide *Superior Replacement for Alexa Fluor 750 tyramide*
iFluor® 790 Styramide *Superior Replacement for Alexa Fluor 790 tyramide*
iFluor® 555 Tyramide
iFluor® 647 Tyramide
iFluor® 350 PSA™ Imaging Kit with Goat Anti-Rabbit IgG
iFluor® 488 PSA™ Imaging Kit with Goat Anti-Rabbit IgG
iFluor® 555 PSA™ Imaging Kit with Goat Anti-Rabbit IgG
iFluor® 594 PSA™ Imaging Kit with Goat Anti-Rabbit IgG
iFluor® 647 PSA™ Imaging Kit with Goat Anti-Rabbit IgG
iFluor 350™ PSA™ Imaging Kit with Goat Anti-Mouse IgG
iFluor 488™ PSA™ Imaging Kit with Goat Anti-Mouse IgG
iFluor 555™ PSA™ Imaging Kit with Goat Anti-Mouse IgG
iFluor 594™ PSA™ Imaging Kit with Goat Anti-Mouse IgG
iFluor 647™ PSA™ Imaging Kit with Goat Anti-Mouse IgG
iFluor® 840 acid
iFluor® 488-Wheat Germ Agglutinin (WGA) Conjugate
iFluor® 555-Wheat Germ Agglutinin (WGA) Conjugate
iFluor® 594-Wheat Germ Agglutinin (WGA) Conjugate
iFluor® 647-Wheat Germ Agglutinin (WGA) Conjugate
iFluor® 488-Concanavalin A Conjugate
iFluor® 555-Concanavalin A Conjugate
iFluor® 594-Concanavalin A Conjugate
iFluor® 647-Concanavalin A Conjugate
iFluor® 350 Tyramide
iFluor® 546 Tyramide
iFluor® 568 Tyramide
iFluor® 594 Tyramide
iFluor® 460 maleimide
iFluor® 488 TCO
iFluor® 555 TCO
iFluor® 594 TCO
iFluor® 647 TCO
iFluor® 488 Tetrazine
iFluor® 555 Tetrazine
iFluor® 594 Tetrazine
iFluor® 647 Tetrazine
iFluor® 440-dUTP *1 mM in Tris Buffer (pH 7.5)*
iFluor® 665 maleimide
iFluor®488-dUTP *1 mM in Tris Buffer (pH 7.5)*
iFluor® 450 Styramide *Superior Replacement for Opal Polaris 480*
iFluor® 633 tyramide
iFluor® 514 Styramide *Superior Replacement for Opal 540*
iFluor® 532 Styramide
iFluor® 633 Styramide *Superior Replacement for Opal 650*
iFluor® 440 Styramide
iFluor® 460 Styramide
iFluor® 610 Styramide
iFluor® 660 Styramide
iFluor® 405 Styramide
iFluor®488-PEG12-dUTP *1 mM in Tris Buffer (pH 7.5)*
iFluor®555-PEG12-dUTP *1 mM in Tris Buffer (pH 7.5)*
iFluor®647-PEG12-dUTP *1 mM in Tris Buffer (pH 7.5)*
iFluor® 800 goat anti-mouse IgG (H+L)
iFluor® 800 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 810 goat anti-mouse IgG (H+L)
iFluor® 810 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 820 goat anti-mouse IgG (H+L)
iFluor® 820 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 840 goat anti-mouse IgG (H+L)
iFluor® 840 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 860 goat anti-mouse IgG (H+L)
iFluor® 860 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 800 goat anti-rabbit IgG (H+L)
iFluor® 800 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 810 goat anti-rabbit IgG (H+L)
iFluor® 810 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 820 goat anti-rabbit IgG (H+L)
iFluor® 820 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 840 goat anti-rabbit IgG (H+L)
iFluor® 840 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 860 goat anti-rabbit IgG (H+L)
iFluor® 860 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 430 Tyramide *Superior Replacement for Opal 480*
iFluor® 450 Tyramide *Superior Replacement for Opal 480*
iFluor® 546 maleimide
iFluor® 840 maleimide
iFluor® 770 maleimide
iFluor® 780 maleimide
iFluor® 350 succinimidyl ester
iFluor® 405 succinimidyl ester
iFluor® 488 succinimidyl ester
iFluor® 514 succinimidyl ester
iFluor® 532 succinimidyl ester
iFluor® 555 succinimidyl ester
iFluor® 594 succinimidyl ester
iFluor® 633 succinimidyl ester
iFluor® 647 succinimidyl ester
iFluor® 660 succinimidyl ester
iFluor® 680 succinimidyl ester
iFluor® 700 succinimidyl ester
iFluor® 750 succinimidyl ester
iFluor® 610 succinimidyl ester
iFluor® 710 succinimidyl ester
iFluor® 790 succinimidyl ester
iFluor® 800 succinimidyl ester
iFluor® 810 succinimidyl ester
iFluor® 820 succinimidyl ester
iFluor® 860 succinimidyl ester
iFluor® 546 succinimidyl ester
iFluor® 568 succinimidyl ester
iFluor® 430 succinimidyl ester
iFluor® 450 succinimidyl ester
iFluor® 840 succinimidyl ester
iFluor® 560 succinimidyl ester
iFluor® 670 succinimidyl ester
iFluor® 460 succinimidyl ester
iFluor® 440 succinimidyl ester
iFluor® 665 succinimidyl ester
iFluor® 690 succinimidyl ester
iFluor® Ultra 594 succinimidyl ester
iFluor® Ultra 647 succinimidyl ester
iFluor® Ultra 750 succinimidyl ester
iFluor® 720 succinimidyl ester
iFluor® 740 succinimidyl ester
iFluor® 597 succinimidyl ester
iFluor® 770 succinimidyl ester
iFluor® 780 succinimidyl ester
iFluor® 570 succinimidyl ester
iFluor® 830 acid
iFluor® 830 maleimide
iFluor® 830 succinimidyl ester
iFluor™ 405 azide
iFluor® 514 maleimide
iFluor® 660 maleimide
iFluor® 670 maleimide
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RPE-iFluor® 750-streptavidin conjugate
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Texas Red®-streptavidin conjugate
Cy3®-streptavidin conjugate
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mFluor™ Violet 510-streptavidin conjugate
mFluor™ Violet 540-streptavidin conjugate
mFluor™ Blue 570-streptavidin conjugate
mFluor™ Green 620-streptavidin conjugate
mFluor™ Yellow 630-streptavidin conjugate
mFluor™ Red 700-streptavidin conjugate
mFluor™ Red 780-streptavidin conjugate
XFD350-streptavidin conjugate *XFD350 Same Structure to Alexa Fluor™ 350*
XFD488-streptavidin conjugate *XFD488 Same Structure to Alexa Fluor™ 488*
XFD594-streptavidin conjugate *XFD594 Same Structure to Alexa Fluor™ 594*
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Show More (276)

OverviewpdfSDSpdfProtocol


Molecular weight
52000
Correction Factor (260 nm)
0.1
Correction Factor (280 nm)
0.14
Extinction coefficient (cm -1 M -1)
2500001
Excitation (nm)
853
Emission (nm)
878
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. A variety of the complementary biotinylated reagents are available from numerous commercial vendors. This iFluor® 860-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with iFluor® 860 covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. iFluor® 860-streptavidin conjugates has fluorescence excitation and emission maxima of ~852 nm and ~877 nm respectively. These spectral characteristics make it an excellent alternative to IRDye® 800CW (IRDye® 800CW is the trademark of Li-COR) due to its much longer excitation wavelength (making it much more tissue-penetrating than IRDye® 800CW). It is a very valuable tool for biotin-streptavidin-based biological assays and tests.

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of iFluor® 860-streptavidin conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM1.923 µL9.615 µL19.231 µL96.154 µL192.308 µL
5 mM384.615 nL1.923 µL3.846 µL19.231 µL38.462 µL
10 mM192.308 nL961.538 nL1.923 µL9.615 µL19.231 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Correction Factor (260 nm)0.1
Correction Factor (280 nm)0.14
Extinction coefficient (cm -1 M -1)2500001
Excitation (nm)853
Emission (nm)878

Product family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor® 350-streptavidin conjugate3454502000010.9510.830.23
iFluor® 405-streptavidin conjugate4034273700010.9110.480.77
iFluor® 488-streptavidin conjugate4915167500010.910.210.11
iFluor® 514-streptavidin conjugate5115277500010.8310.2650.116
iFluor® 532-streptavidin conjugate5375609000010.6810.260.16
iFluor® 555-streptavidin conjugate55757010000010.6410.230.14
iFluor® 594-streptavidin conjugate58860418000010.5310.050.04
iFluor® 633-streptavidin conjugate64065425000010.2910.0620.044
iFluor® 647-streptavidin conjugate65667025000010.2510.030.03
iFluor® 680-streptavidin conjugate68470122000010.2310.0970.094
iFluor® 700-streptavidin conjugate69071322000010.2310.090.04
iFluor® 750-streptavidin conjugate75777927500010.1210.0440.039
iFluor® 546-streptavidin conjugate54155710000010.6710.250.15
iFluor® 568-streptavidin conjugate56858710000010.5710.340.15
iFluor® 790-streptavidin conjugate78781225000010.1310.10.09
iFluor® 800-streptavidin conjugate80182025000010.1110.030.08
iFluor® 820-streptavidin conjugate8228502500001-0.110.16
iFluor® 840-streptavidin conjugate8368792000001-0.20.09
Show More (19)

Citations


View all 15 citations: Citation Explorer
Aptamer Structure Switch Fluorescence Anisotropy Assay for Small Molecules Using Streptavidin as an Effective Signal Amplifier Based on Proximity Effect
Authors: Li, Y., Zhao, Q.
Journal: Anal Chem (2019): 7379-7384
Streptavidin cooperative allosterism upon binding biotin observed by differential changes in intrinsic fluorescence
Authors: Waner, M. J., Hiznay, J. M., Mustovich, A. T., Patton, W., Ponyik, C., Mascotti, D. P.
Journal: Biochem Biophys Rep (2019): 127-131
Streptavidin interfacing as a general strategy to localize fluorescent membrane tension probes in cells
Authors: Goujon, A., Strakova, K., Sakai, N., Matile, S.
Journal: Chem Sci (2019): 310-319
Streptavidin-conjugated gold nanoclusters as ultrasensitive fluorescent sensors for early diagnosis of HIV infection
Authors: Kurdekar, A. D., Avinash Chunduri, L. A., Manohar, C. S., Haleyurgirisetty, M. K., Hewlett, I. K., Venkataramaniah, K.
Journal: Sci Adv (2018): eaar6280
Design and Fabrication of Streptavidin-Functionalized, Fluorescently Labeled Polymeric Nanocarriers
Authors: Jo, A., Zhang, R., Allen, I. C., Riffle, J. S., Davis, R. M.
Journal: Langmuir (2018): 15783-15794
Optimized Streptavidin for Fluorescent Labeling of Biotinylated Targets
Authors: Hytonen, V. P.
Journal: Cell Chem Biol (2017): 921-922
Quantum dot-fluorescence in situ hybridisation for Ectromelia virus detection based on biotin-streptavidin interactions
Authors: Wang, T., Zheng, Z., Zhang, X. E., Wang, H.
Journal: Talanta (2016): 179-184
Streptavidin-triggered signal amplified fluorescence polarization for analysis of DNA-protein interactions
Authors: Choi, J. W., Jo, B. G., deMello, A. J., Choo, J., Kim, H. Y.
Journal: Analyst (2016): 6499-6502
Novel biosensor system model based on fluorescence quenching by a fluorescent streptavidin and carbazole-labeled biotin
Authors: Zhu, X., Shinohara, H., Miyatake, R., Hohsaka, T.
Journal: J Mol Recognit (2016): 485-91
Discrimination between streptavidin and avidin with fluorescent affinity-based probes
Authors: Sun, Q., Tian, H., Qu, H., Sun, D., Chen, Z., Duan, L., Zhang, W., Qian, J.
Journal: Analyst (2015): 4648-53

References


View all 1 references: Citation Explorer
Aluminum phthalocyanine-streptavidin: new, sensitive fluorescent tracer for immunoassay
Authors: Kelly TA, Hunter CA, Schindele DC, Pepich BV.
Journal: Clin Chem (1991): 1283