The Power Styramide™ Signal Amplification (PSA™) system is an advanced detection platform designed to achieve ultra-sensitive visualization of low-abundance targets in cells and tissues. PSA™ enhances fluorescence signal intensity by 10- to 50-fold compared to conventional tyramide signal amplification (TSA) methods. When combined with our high-performance iFluor® dyes, which exhibit superior quantum yield, enhanced photostability, and increased aqueous solubility, iFluor® dye-labeled Styramide™ conjugates provide fluorescence signals with over 100-fold greater sensitivity and spatial resolution compared to standard immunocytochemistry (ICC), immunofluorescence (IF), and immunohistochemistry (IHC) techniques.

The PSA™ system utilizes the catalytic activity of horseradish peroxidase (HRP) to mediate the covalent deposition of fluorophores onto target sites. PSA radicals exhibit higher reactivity compared to traditional tyramide radicals, leading to faster reaction kinetics and improved labeling efficiency. This results in increased fluorescence signal intensity and enhanced detection sensitivity for low-abundance targets.

Styramide™ conjugates also allow for a substantial reduction in primary antibody usage while maintaining or surpassing the sensitivity of direct conjugation and traditional TSA-based methodologies. iFluor® 860 Styramide provides emission properties within the near-infrared (NIR) spectral range, facilitating deeper tissue penetration, lower autofluorescence, and enhanced signal-to-noise ratios. These characteristics make it particularly well-suited for applications requiring high sensitivity in complex biological environments, such as in vivo imaging and multiplexed fluorescence detection.