Indo-1 AM | AAT Bioquest

Indo-1, AM

CAS 112926-02-0

Indo-1 AM is a UV-excitable, cell-permeable ratiometric calcium indicator that shifts emission upon calcium binding, enabling quantitative measurement of intracellular calcium-ion dynamics.

Ratiometric detection: Distinct shift in emission from 480 nm to 400 nm upon binding to calcium
Optimized for flow cytometry: Requires only a single UV excitation source, such as 351–364 nm spectral lines, simplifying experimental setups
Versatile applications: Compatible with various techniques, including fluorescence microscopy, spectroscopy, microplate readers, and flow cytometry

Fluorescence emission spectra of Indo-1 in solutions conctaining 0 to 39uM free Ca2+.

Protocol

COO

COA

Catalog	Size	Price	Quantity
21030	1 mg	Price
21033	50 mg	Price

Overview

Indo-1 AM is based on the principle of a distinct shift in emission upon binding to Ca²⁺, transitioning from 480 nm (in the absence of Ca²⁺) to 400 nm (when bound to Ca²⁺). Indo-1 AM's high calcium-binding affinity (K_d ~230 nM) makes it highly sensitive to transient calcium ion changes within the cell. Indo-1 AM enables quantitative detection of calcium flux dynamics through real-time emission ratio measurements. The excitation and emission characteristics of Indo-1 AM makes it ideal for multiple applications like fluorescence microscopy, spectroscopy, microplate readers and flow cytometry in particular, as it only requires a single UV excitation source, such as the 351–364 nm lines from an argon-ion laser channel. It delivers superior performance with minimal background, ensuring reliable and reproducible results for complex cellular assays.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Indo-1, AM *CAS 112926-02-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	99.019 µL	495.094 µL	990.187 µL	4.951 mL	9.902 mL
5 mM	19.804 µL	99.019 µL	198.037 µL	990.187 µL	1.98 mL
10 mM	9.902 µL	49.509 µL	99.019 µL	495.094 µL	990.187 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1009.91 g/mol	=	mL	x	mM	=	mmol

Citations

View all 6 citations: Citation Explorer

Modeling the atrioventricular conduction axis using human pluripotent stem cell-derived cardiac assembloids

Authors:

Li, Jiuru and Wiesinger, Alexandra and Fokkert, Lianne and Bakker, Priscilla and de Vries, Dylan K and Tijsen, Anke J and Pinto, Yigal M and Verkerk, Arie O and Christoffels, Vincent M and Boink, Gerard JJ and others,

Journal:

Cell Stem Cell (2024)

Development of a deep two-photon calcium imaging method for the analysis of cortical processing in the mammalian brain

Authors:

Birkner, Antje

Nifedipine stimulates proliferation and migration of different breast cancer cells by distinct pathways

Authors:

Zhao, Tao and Guo, Dongqing and Gu, Yuchun and Ling, Yang

Journal:

Molecular Medicine Reports (2017): 2259--2263

Inhibition of pyruvate kinase M2 by reactive oxygen species contributes to the development of pulmonary arterial hypertension

Authors:

Guo, Dongqing and Gu, Junzhong and Jiang, Hui and Ahmed, Asif and Zhang, Zhiren and Gu, Yuchun

Journal:

Journal of molecular and cellular cardiology (2016): 179--187

Nifedipine promotes the proliferation and migration of breast cancer cells

Authors:

Guo, Dong-Qing and Zhang, Hao and Tan, Sheng-Jiang and Gu, Yu-Chun

Journal:

PloS one (2014): e113649

References

View all 84 references: Citation Explorer

Measurement of [Ca2+] in cell suspensions using indo-1

Authors:

Nelemans A., undefined

Journal:

Methods Mol Biol (2006): 47

A flow cytometric comparison of Indo-1 to fluo-3 and Fura Red excited with low power lasers for detecting Ca(2+) flux

Authors:

Bailey S, Macardle PJ.

Journal:

J Immunol Methods (2006): 220

Ratiometric intracellular calcium imaging in the isolated beating rat heart using indo-1 fluorescence

Authors:

Eerbeek O, Mik EG, Zuurbier CJ, van 't Loo M, Donkersloot C, Ince C.

Journal:

J Appl Physiol (2004): 2042

Negative inotropic effects of angiotensin II, endothelin-1 and phenylephrine in indo-1 loaded adult mouse ventricular myocytes

Authors:

Sakurai K, Norota I, Tanaka H, Kubota I, Tomoike H, Endo M.

Journal:

Life Sci (2002): 1173

Usefulness of the analytic method of intracellular calcium and the problems--aequorin and indo-1 signal

Authors:

Endoh M., undefined

Journal:

Nippon Yakurigaku Zasshi (2000): 361

Physical properties

Dissociation constant (K_d, nM)	230
Molecular weight	1009.91
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	33000
Excitation (nm)	330
Emission (nm)	404

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	112926-02-0

Instrument settings

Fluorescence microscope
Excitation	Indo-1 filter set
Emission	Indo-1 filter set
Recommended plate	Black wall/clear bottom

Fluorescence microplate reader
Excitation	340
Emission	400, 475
Cutoff	Ex/Em = 340/400, no cut off. Ex/Em = 340/475, cut off 455
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode/Programmable liquid handling

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Page updated on September 20, 2024