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JUN Dye qPCR Calibration Solution *10,000X*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Solvent | Water |
Spectral properties
| Extinction coefficient (cm -1 M -1) | 1100001 |
| Excitation (nm) | 610 |
| Emission (nm) | 628 |
| Quantum yield | 0.851 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
| Overview |  SDSProtocol |
Extinction coefficient (cm -1 M -1) 1100001 | Excitation (nm) 610 | Emission (nm) 628 | Quantum yield 0.851 |
Real-time thermal cyclers require calibration to generate a spectral profile for the fluorescence signal. Since instruments can differ in fluorescence detection sensitivity, the concentrations needed to calibrate any dye, such as JUN, will depend on the device used. The AAT Bioquest JUN dye qPCR Calibration Solution supplied as a 10,000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ 7 systems. Calibration and verification should be run every six months or as needed. Please refer to the instrument maintenance guide for complete instructions on use.
Platform
qPCR
| Recommended plate | PCR Microplate |
| Instrument specification(s) | JUN Filter |
Example protocol
SAMPLE EXPERIMENTAL PROTOCOL
This generic protocol is suitable for the ABI7500 FAST qPCR instrument. For complete instructions on use, refer to the maintenance guide for your instrument.
Prepare a JUN calibration plate:
Prepare a JUN Dye qPCR Calibration working solution by mixing 1 µL of the 10,000X JUN Dye qPCR Calibration Solution with 10 mL of nuclease-free water or qPCR buffer.
Note: The final dye concentration must be optimized based on the calibration requirements for your instrument. Refer to the manufacturer’s maintenance guide for detailed instructions.
Add 20 µL of the JUN Dye qPCR Calibration working solution to all wells of a reaction plate.
Note: The volume may vary depending on your instrument. Refer to the manufacturer’s maintenance guide for detailed instructions.
Seal the plate with an optical adhesive cover.
Centrifuge the plate for 2 minutes at < 1500 rpm.
Check that the liquid in each well of the plate is at the bottom of the well. If not, centrifuge the plate again at a slightly higher rpm and for a longer period of time.
For complete calibration instructions, refer to the maintenance guide for your instrument.
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Extinction coefficient (cm -1 M -1) | 1100001 |
| Excitation (nm) | 610 |
| Emission (nm) | 628 |
| Quantum yield | 0.851 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| VIC Dye qPCR Calibration Solution *10,000X* | 526 | 543 | - |
| NED Dye qPCR Calibration Solution *10,000X* | 545 | 567 | 740001 |
| FAM Dye qPCR Calibration Solution *10,000X* | 493 | 517 | 83000 |
| Cy5 Dye qPCR Calibration Solution *10,000X* | 651 | 670 | 2500001 |
| ROX Dye qPCR Calibration Solution *10,000X* | 578 | 595 | - |
| SYBR Dye qPCR Calibration Solution *10,000X* | 498 | 522 | - |
| TAMRA Dye qPCR Calibration Solution *10,000X* | 552 | 578 | 90000 |
| ABY Dye qPCR Calibration Solution *10,000X* | 559 | 570 | 1200001 |
| MP650 Dye qPCR Calibration Solution *10,000X* | 639 | 653 | 2500001 |
Show More (1) | |||
Images
Figure 1. ABI 7500 FAST system JUN dye spectrum.
References
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Integration of Single-Cell and Bulk RNA-seq Data to Identify the Cancer-Associated Fibroblast Subtypes and Risk Model in Glioma.
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Mitochondrial energy metabolism-related gene signature as a prognostic indicator for pancreatic adenocarcinoma.
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Journal: Frontiers in pharmacology (2024): 1332042
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Journal: Frontiers in pharmacology (2024): 1332042
Prognostic stratification of sepsis through DNA damage response based RiskScore system: insights from single-cell RNA-sequencing and transcriptomic profiling.
Authors: Lin, Qingjiang and Zeng, Rongyao and Yang, Jinfeng and Xu, Zebo and Jin, Shaoxiong and Wei, Guan
Journal: Frontiers in immunology (2024): 1345321
Authors: Lin, Qingjiang and Zeng, Rongyao and Yang, Jinfeng and Xu, Zebo and Jin, Shaoxiong and Wei, Guan
Journal: Frontiers in immunology (2024): 1345321
Comparison of quantitative PCR and digital PCR assays for quantitative detection of infectious bronchitis virus (IBV) genome.
Authors: Isham, Ishara M and Najimudeen, Shahnas M and Cork, Susan C and Gupta, Ashish and Abdul-Careem, Mohamed Faizal
Journal: Journal of virological methods (2024): 114859
Authors: Isham, Ishara M and Najimudeen, Shahnas M and Cork, Susan C and Gupta, Ashish and Abdul-Careem, Mohamed Faizal
Journal: Journal of virological methods (2024): 114859
Exploration of Diagnostic Deubiquitinating Enzymes in Endometriosis and Its Immune Infiltration.
Authors: Yang, Xinyun and Yan, Kai and Zhan, Qitao and Chen, Hao and Pei, Chang-Zhu and Zhu, Linling
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Screening immune-related blood biomarkers for DKD-related HCC using machine learning.
Authors: Chen, Chao and Xie, Zhinan and Ni, Ying and He, Yuxi
Journal: Frontiers in immunology (2024): 1339373
Authors: Chen, Chao and Xie, Zhinan and Ni, Ying and He, Yuxi
Journal: Frontiers in immunology (2024): 1339373
An Efficient Probe-Based Quantitative PCR Assay Targeting Human-Specific DNA in ST6GALNAC3 for the Quantification of Human Cells in Preclinical Animal Models.
Authors: Ren, Jinfeng and Liu, Ke and Hu, Lang and Yang, Ruoning and Liu, Yuting and Wang, Siyu and Chen, Xinzhu and Zhao, Shuli and Jing, Luyao and Liu, Tiantian and Hu, Bin and Zhang, Xuefeng and Wang, Hui and Li, Hui
Journal: Molecular biotechnology (2024)
Authors: Ren, Jinfeng and Liu, Ke and Hu, Lang and Yang, Ruoning and Liu, Yuting and Wang, Siyu and Chen, Xinzhu and Zhao, Shuli and Jing, Luyao and Liu, Tiantian and Hu, Bin and Zhang, Xuefeng and Wang, Hui and Li, Hui
Journal: Molecular biotechnology (2024)
Internationally standardized respiratory viral load testing with limited resources: A derivative-of-care calibration strategy for SARS-CoV-2.
Authors: Tao, Lili and Chan, Allison and Maris, Alex and Schmitz, Jonathan E
Journal: Influenza and other respiratory viruses (2024): e13207
Authors: Tao, Lili and Chan, Allison and Maris, Alex and Schmitz, Jonathan E
Journal: Influenza and other respiratory viruses (2024): e13207