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AAT Bioquest

JUN Dye qPCR Calibration Solution *10,000X*

Real-time thermal cyclers require calibration to generate a spectral profile for the fluorescence signal. Since instruments can differ in fluorescence detection sensitivity, the concentrations needed to calibrate any dye, such as JUN, will depend on the device used. The AAT Bioquest JUN dye qPCR Calibration Solution supplied as a 10,000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ 7 systems. Calibration and verification should be run every six months or as needed. Please refer to the instrument maintenance guide for complete instructions on use.

Example protocol

SAMPLE EXPERIMENTAL PROTOCOL

This generic protocol is suitable for the ABI7500 FAST qPCR instrument. For complete instructions on use, refer to the maintenance guide for your instrument.

Prepare a JUN calibration plate:
  1. Prepare a JUN Dye qPCR Calibration working solution by mixing 1 µL of the 10,000X JUN Dye qPCR Calibration Solution with 10 mL of nuclease-free water or qPCR buffer.

    Note: The final dye concentration must be optimized based on the calibration requirements for your instrument. Refer to the manufacturer’s maintenance guide for detailed instructions.

  2. Add 20 µL of the JUN Dye qPCR Calibration working solution to all wells of a reaction plate.

    Note: The volume may vary depending on your instrument. Refer to the manufacturer’s maintenance guide for detailed instructions.

  3. Seal the plate with an optical adhesive cover.

  4. Centrifuge the plate for 2 minutes at < 1500 rpm.

  5. Check that the liquid in each well of the plate is at the bottom of the well. If not, centrifuge the plate again at a slightly higher rpm and for a longer period of time.

  6. For complete calibration instructions, refer to the maintenance guide for your instrument.

Spectrum

References

View all 50 references: Citation Explorer
MAP4 acts as an oncogene and prognostic marker and affects radioresistance by mediating epithelial-mesenchymal transition in lung adenocarcinoma.
Authors: Xia, Xiaochun and Ge, Yangyang and Ge, Fanghong and Gu, Pei and Liu, Yuanyuan and Li, Peng and Xu, Pengqin
Journal: Journal of cancer research and clinical oncology (2024): 88
Integration of Single-Cell and Bulk RNA-seq Data to Identify the Cancer-Associated Fibroblast Subtypes and Risk Model in Glioma.
Authors: Yan, Xiuwei and Gao, Xin and Dong, Jiawei and Wang, Fang and Jiang, Xiaoyan and Hu, Xueyan and Zhang, Jiheng and Wang, Nan and Xu, Lei and Liu, Zhihui and Hu, Shaoshan and Zhao, Hongtao
Journal: Biochemical genetics (2024)
Novel Prognostic Model Construction of Tongue Squamous Cell Carcinoma Based on Apigenin-Associated Genes.
Authors: Lai, Jianfei and Fang, Chen and Zhang, Guohua and Shi, Chao and Yu, Feng and Gu, Weiguo and Deng, Jianxiong and Xu, Jingbiao and Liu, Chaoxing and Qiu, Feng
Journal: Frontiers in bioscience (Landmark edition) (2024): 65
Mitochondrial energy metabolism-related gene signature as a prognostic indicator for pancreatic adenocarcinoma.
Authors: Ma, Yu and Tang, Ronghao and Huang, Peilin and Li, Danhua and Liao, Meijian and Gao, Shoucui
Journal: Frontiers in pharmacology (2024): 1332042
Prognostic stratification of sepsis through DNA damage response based RiskScore system: insights from single-cell RNA-sequencing and transcriptomic profiling.
Authors: Lin, Qingjiang and Zeng, Rongyao and Yang, Jinfeng and Xu, Zebo and Jin, Shaoxiong and Wei, Guan
Journal: Frontiers in immunology (2024): 1345321
Page updated on November 7, 2024

Ordering information

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Catalog Number67038
Quantity
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Physical properties

Solvent

Water

Spectral properties

Extinction coefficient (cm -1 M -1)

1100001

Excitation (nm)

610

Emission (nm)

628

Quantum yield

0.851

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

Platform

qPCR

Recommended platePCR Microplate
Instrument specification(s)JUN Filter
ABI 7500 FAST system JUN dye spectrum.
ABI 7500 FAST system JUN dye spectrum.
ABI 7500 FAST system JUN dye spectrum.