LAMP Green™ *50X DMSO Solution*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 578.73 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Overview | ![]() ![]() |
Molecular weight 578.73 |
Isothermal amplification methods provide detection of nucleic acid target sequence in a streamlined, exponential manner. The existing dyes that successfully detect the LAMP reaction are mostly based on the end point method using the colorimetric analysis. LAMP Green™ enables real-time fluorescence measurement of a LAMP reaction. The dye can be detected using the SYBR or FAM channel on common real-time PCR instruments. LAMP Green™ is compatible with the agarose gel electrophoresis, making it possible to run on a gel and analyze it by gel imager.
Example protocol
SAMPLE EXPERIMENTAL PROTOCOL
Table 1. 25 µL LAMP reaction mix.
Components | DNA/RNA target detection | No Template Control (NTC) |
LAMP master mix (2X) | 12.5 µL | 12.5 µL |
LAMP primer mix (10X) | 2.5 µL | 2.5 µL |
LAMP Green™ (50X) | 0.5 µL | 0.5 µL |
Target DNA/RNA | Varies | 0 µL |
ddH2O | Varies | 9.5 µL |
Total Volume | 25 µL | 25 µL |
- Thaw all components to be used at room temperature and place on ice. Vortex briefly to mix and centrifuge to collect material.
- Prepare reaction mix as described in Table 1. Volumes are listed for 25 µL/LAMP reaction. Adjust volume accordingly as per use. If necessary, LAMP Green™ concentration can be optimized.
- Vortex reaction mix and centrifuge to collect material.
- Seal the reaction vessel.
- Incubate at 65°C for 30 minutes. If necessary, time can be extended (i.e., for low copy targets, challenging sample types, or reactions known to produce slower amplification times).
- If using with a qPCR machine, the signal can be read with either a SYBR or FAM filter in real-time. For microplate reader-based assays, measure the signal at Ex/Em = 490/525 nm (Cutoff = 515 nm).
- If reaction products will be analyzed after LAMP reaction is completed, then deactivate the enzyme by heating at >80°C for 5 minutes.
Note Deactivation can be performed for LAMP master mix provided by the manufacturer. LAMP Green™ is compatible with agarose gel electrophoresis, so samples can be analyzed on an agarose gel.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of LAMP Green™ *50X DMSO Solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 172.792 µL | 863.961 µL | 1.728 mL | 8.64 mL | 17.279 mL |
5 mM | 34.558 µL | 172.792 µL | 345.584 µL | 1.728 mL | 3.456 mL |
10 mM | 17.279 µL | 86.396 µL | 172.792 µL | 863.961 µL | 1.728 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Product Family
Name | Excitation (nm) | Emission (nm) |
Indocyanine Green *CAS 3599-32-4* | 789 | 814 |
Helixyte™ Green *20X Aqueous PCR Solution* | 498 | 522 |
Helixyte™ Green *10,000X Aqueous PCR Solution* | 498 | 522 |
Thiolite™ Green | 510 | 525 |
CytoTell™ Green | 510 | 525 |
LysoBrite™ Green | 501 | 510 |
Q4ever™ Green *1250X DMSO Solution* | 503 | 527 |
Droplite™ Green | 421 | 521 |
Images
References
View all 16 references: Citation Explorer
Comparison of COVID-19 laboratory diagnosis by commercial kits: Effectivity of RT-PCR to the RT-LAMP.
Authors: Artik, Yakup and Coşğun, Alp B and Cesur, Nevra P and Hızel, Nedret and Uyar, Yavuz and Sur, Haydar and Ayan, Alp
Journal: Journal of medical virology (2022): 1998-2007
Authors: Artik, Yakup and Coşğun, Alp B and Cesur, Nevra P and Hızel, Nedret and Uyar, Yavuz and Sur, Haydar and Ayan, Alp
Journal: Journal of medical virology (2022): 1998-2007
SARS-CoV-2's high rate of genetic mutation under immune selective pressure: from oropharyngeal B.1.1.7 to intrapulmonary B.1.533 in a vaccinated patient.
Authors: Musso, Nicolò and Maugeri, Jessica Giuseppina and Bongiorno, Dafne and Stracquadanio, Stefano and Bartoloni, Giovanni and Stefani, Stefania and Di Stefano, Emanuela Daniela
Journal: International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases (2022): 169-172
Authors: Musso, Nicolò and Maugeri, Jessica Giuseppina and Bongiorno, Dafne and Stracquadanio, Stefano and Bartoloni, Giovanni and Stefani, Stefania and Di Stefano, Emanuela Daniela
Journal: International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases (2022): 169-172
Rapid quantification of fecal indicator bacteria in water using the most probable number - loop-mediated isothermal amplification (MPN-LAMP) approach on a polymethyl methacrylate (PMMA) microchip.
Authors: Fu, Jing and Chiang, Elaine Li Ching and Medriano, Carl Angelo Dulatre and Li, Liyan and Bae, Sungwoo
Journal: Water research (2021): 117172
Authors: Fu, Jing and Chiang, Elaine Li Ching and Medriano, Carl Angelo Dulatre and Li, Liyan and Bae, Sungwoo
Journal: Water research (2021): 117172
Severe long-delayed malaria caused by Plasmodium malariae in an elderly French patient.
Authors: Marteau, Anthony and Ouedraogo, Elise and Van der Meersch, Guillaume and Akhoundi, Mohammad and Souhail, Berenice and Cohen, Yves and Bouchaud, Olivier and Izri, Arezki
Journal: Malaria journal (2021): 337
Authors: Marteau, Anthony and Ouedraogo, Elise and Van der Meersch, Guillaume and Akhoundi, Mohammad and Souhail, Berenice and Cohen, Yves and Bouchaud, Olivier and Izri, Arezki
Journal: Malaria journal (2021): 337
Confirmed validation of an innovative PCR-assay without DNA extraction for multiplex diagnosis of factor V Leiden and prothrombin gene variants.
Authors: Desjonquères, A and Ménard, A and Detemmerman, L and Ternisien, C and Fouassier, M and Gillet, B and Béné, M C and Le Bris, Y
Journal: Thrombosis research (2019): 143-145
Authors: Desjonquères, A and Ménard, A and Detemmerman, L and Ternisien, C and Fouassier, M and Gillet, B and Béné, M C and Le Bris, Y
Journal: Thrombosis research (2019): 143-145
[Clinical Application of Loop-mediated Isothermal Amplification in Detection of Mycoplasma Pneumoniae].
Authors: Yan, Chun Xia and Lu, Wei Hong and He, Guo Chan and Wen, Ren Qing and Qian, Ying
Journal: Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae (2019): 203-207
Authors: Yan, Chun Xia and Lu, Wei Hong and He, Guo Chan and Wen, Ren Qing and Qian, Ying
Journal: Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae (2019): 203-207
Rapid and in-situ detection of fecal indicator bacteria in water using simple DNA extraction and portable loop-mediated isothermal amplification (LAMP) PCR methods.
Authors: Lee, Seunguk and Khoo, Valerie Si Ling and Medriano, Carl Angelo Dulatre and Lee, Taewoo and Park, Sung-Yong and Bae, Sungwoo
Journal: Water research (2019): 371-379
Authors: Lee, Seunguk and Khoo, Valerie Si Ling and Medriano, Carl Angelo Dulatre and Lee, Taewoo and Park, Sung-Yong and Bae, Sungwoo
Journal: Water research (2019): 371-379
Cloning of BBTV (Banana Bunchy Top Virus) components and screening of BBTV using functionalized gold nanoparticles.
Authors: Kumar, P and Arun, V and Lokeswari, T S
Journal: 3 Biotech (2017): 225
Authors: Kumar, P and Arun, V and Lokeswari, T S
Journal: 3 Biotech (2017): 225
Genetic variability and discrimination of low doses of Toxocara spp. from public areas soil inferred by loop-mediated isothermal amplification assay as a field-friendly molecular tool.
Authors: Ozlati, Maryam and Spotin, Adel and Shahbazi, Abbas and Mahami-Oskouei, Mahmoud and Hazratian, Teimour and Adibpor, Mohammad and Ahmadpour, Ehsan and Dolatkhah, Afsaneh and Khoshakhlagh, Paria
Journal: Veterinary world (2016): 1471-1477
Authors: Ozlati, Maryam and Spotin, Adel and Shahbazi, Abbas and Mahami-Oskouei, Mahmoud and Hazratian, Teimour and Adibpor, Mohammad and Ahmadpour, Ehsan and Dolatkhah, Afsaneh and Khoshakhlagh, Paria
Journal: Veterinary world (2016): 1471-1477
LAMP-PCR detection of ochratoxigenic Aspergillus species collected from peanut kernel.
Authors: Al-Sheikh, H M
Journal: Genetics and molecular research : GMR (2015): 634-44
Authors: Al-Sheikh, H M
Journal: Genetics and molecular research : GMR (2015): 634-44