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LAMP Green™ *50X DMSO Solution*
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FAQ
Application notes
Isothermal amplification methods provide detection of nucleic acid target sequence in a streamlined, exponential manner. The existing dyes that successfully detect the LAMP reaction are mostly based on the end point method using the colorimetric analysis. LAMP Green™ enables real-time fluorescence measurement of a LAMP reaction. The dye can be detected using the SYBR or FAM channel on common real-time PCR instruments. LAMP Green™ is compatible with the agarose gel electrophoresis, making it possible to run on a gel and analyze it by gel imager.
Example protocol
SAMPLE EXPERIMENTAL PROTOCOL
Table 1. 25 µL LAMP reaction mix.
| Components | DNA/RNA target detection | No Template Control (NTC) |
| LAMP master mix (2X) | 12.5 µL | 12.5 µL |
| LAMP primer mix (10X) | 2.5 µL | 2.5 µL |
| LAMP Green™ (50X) | 0.5 µL | 0.5 µL |
| Target DNA/RNA | Varies | 0 µL |
| ddH2O | Varies | 9.5 µL |
| Total Volume | 25 µL | 25 µL |
- Thaw all components to be used at room temperature and place on ice. Vortex briefly to mix and centrifuge to collect material.
- Prepare reaction mix as described in Table 1. Volumes are listed for 25 µL/LAMP reaction. Adjust volume accordingly as per use. If necessary, LAMP Green™ concentration can be optimized.
- Vortex reaction mix and centrifuge to collect material.
- Seal the reaction vessel.
- Incubate at 65°C for 30 minutes. If necessary, time can be extended (i.e., for low copy targets, challenging sample types, or reactions known to produce slower amplification times).
- If using with a qPCR machine, the signal can be read with either a SYBR or FAM filter in real-time. For microplate reader-based assays, measure the signal at Ex/Em = 490/525 nm (Cutoff = 515 nm).
- If reaction products will be analyzed after LAMP reaction is completed, then deactivate the enzyme by heating at >80°C for 5 minutes.
Note Deactivation can be performed for LAMP master mix provided by the manufacturer. LAMP Green™ is compatible with agarose gel electrophoresis, so samples can be analyzed on an agarose gel.
Product family
| Name | Excitation (nm) | Emission (nm) |
| Indocyanine Green *CAS 3599-32-4* | 789 | 813 |
| Helixyte™ Green *20X Aqueous PCR Solution* | 498 | 522 |
| Helixyte™ Green *10,000X Aqueous PCR Solution* | 498 | 522 |
| Thiolite™ Green | 505 | 524 |
| CytoTell™ Green | 510 | 525 |
| LysoBrite™ Green | 501 | 510 |
| Q4ever™ Green *1250X DMSO Solution* | 503 | 527 |
| Droplite™ Green | 421 | 521 |
| FerroBrite™ Green | 453 | 552 |
References
View all 16 references: Citation Explorer
SARS-CoV-2's high rate of genetic mutation under immune selective pressure: from oropharyngeal B.1.1.7 to intrapulmonary B.1.533 in a vaccinated patient.
Authors: Musso, Nicolò and Maugeri, Jessica Giuseppina and Bongiorno, Dafne and Stracquadanio, Stefano and Bartoloni, Giovanni and Stefani, Stefania and Di Stefano, Emanuela Daniela
Journal: International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases (2022): 169-172
Authors: Musso, Nicolò and Maugeri, Jessica Giuseppina and Bongiorno, Dafne and Stracquadanio, Stefano and Bartoloni, Giovanni and Stefani, Stefania and Di Stefano, Emanuela Daniela
Journal: International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases (2022): 169-172
Comparison of COVID-19 laboratory diagnosis by commercial kits: Effectivity of RT-PCR to the RT-LAMP.
Authors: Artik, Yakup and Coşğun, Alp B and Cesur, Nevra P and Hızel, Nedret and Uyar, Yavuz and Sur, Haydar and Ayan, Alp
Journal: Journal of medical virology (2022): 1998-2007
Authors: Artik, Yakup and Coşğun, Alp B and Cesur, Nevra P and Hızel, Nedret and Uyar, Yavuz and Sur, Haydar and Ayan, Alp
Journal: Journal of medical virology (2022): 1998-2007
Severe long-delayed malaria caused by Plasmodium malariae in an elderly French patient.
Authors: Marteau, Anthony and Ouedraogo, Elise and Van der Meersch, Guillaume and Akhoundi, Mohammad and Souhail, Berenice and Cohen, Yves and Bouchaud, Olivier and Izri, Arezki
Journal: Malaria journal (2021): 337
Authors: Marteau, Anthony and Ouedraogo, Elise and Van der Meersch, Guillaume and Akhoundi, Mohammad and Souhail, Berenice and Cohen, Yves and Bouchaud, Olivier and Izri, Arezki
Journal: Malaria journal (2021): 337
Rapid quantification of fecal indicator bacteria in water using the most probable number - loop-mediated isothermal amplification (MPN-LAMP) approach on a polymethyl methacrylate (PMMA) microchip.
Authors: Fu, Jing and Chiang, Elaine Li Ching and Medriano, Carl Angelo Dulatre and Li, Liyan and Bae, Sungwoo
Journal: Water research (2021): 117172
Authors: Fu, Jing and Chiang, Elaine Li Ching and Medriano, Carl Angelo Dulatre and Li, Liyan and Bae, Sungwoo
Journal: Water research (2021): 117172
Rapid and in-situ detection of fecal indicator bacteria in water using simple DNA extraction and portable loop-mediated isothermal amplification (LAMP) PCR methods.
Authors: Lee, Seunguk and Khoo, Valerie Si Ling and Medriano, Carl Angelo Dulatre and Lee, Taewoo and Park, Sung-Yong and Bae, Sungwoo
Journal: Water research (2019): 371-379
Authors: Lee, Seunguk and Khoo, Valerie Si Ling and Medriano, Carl Angelo Dulatre and Lee, Taewoo and Park, Sung-Yong and Bae, Sungwoo
Journal: Water research (2019): 371-379