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LDS 751 *CAS 181885-68-7*
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Application notes
LDS 751 is a fluorescent cell-permeant nucleic acid stain that can be well excited with 488 nm laser line although it has a peak excitation at ~543 nm on dsDNA. It might be an alternative to DRAQ 5™, useful in multicolor analyses due to its long wavelength emission maximum (~712 nm). Upon binding to dsDNA of LDS 751 has ~20-fold fluorescence enhancement. It has been reported that LDS-751 is excluded from the nucleus and binds the polarized membranes of mitochondria. Thus cautions need be taken when LDS 751 is used for analyzing live cells and LDS-751 fluorescence as being indicative of nuclear status. Thiazole orange (an RNA dye) and LDS-751 (a DNA dye) composition has been used to separate reticulocytes from other cell populations. LDS 751 has been also used to separate red blood cells from nucleated cells.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of LDS 751 *CAS 181885-68-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 211.873 µL | 1.059 mL | 2.119 mL | 10.594 mL | 21.187 mL |
| 5 mM | 42.375 µL | 211.873 µL | 423.747 µL | 2.119 mL | 4.237 mL |
| 10 mM | 21.187 µL | 105.937 µL | 211.873 µL | 1.059 mL | 2.119 mL |
Molarity calculator
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| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) |
| LDS 651 | 557 | 630 |
References
View all 29 references: Citation Explorer
Interaction of LDS-751 with the drug-binding site of P-glycoprotein: a Trp fluorescence steady-state and lifetime study
Authors: Lugo MR, Sharom FJ.
Journal: Arch Biochem Biophys (2009): 17
Authors: Lugo MR, Sharom FJ.
Journal: Arch Biochem Biophys (2009): 17
Hybridization-sensitive fluorescent probe for long-term monitoring of intracellular RNA
Authors: Kubota T, Ikeda S, Yanagisawa H, Yuki M, Okamoto A.
Journal: Bioconjug Chem (2009): 1256
Authors: Kubota T, Ikeda S, Yanagisawa H, Yuki M, Okamoto A.
Journal: Bioconjug Chem (2009): 1256
Exciton-controlled fluorescence: application to hybridization-sensitive fluorescent DNA probe
Authors: Okamoto A, Ikeda S, Kubota T, Yuki M, Yanagisawa H.
Journal: Nucleic Acids Symp Ser (Oxf) (2009): 49
Authors: Okamoto A, Ikeda S, Kubota T, Yuki M, Yanagisawa H.
Journal: Nucleic Acids Symp Ser (Oxf) (2009): 49
Flow cytometric assessment of homeostatic aging of reticulocytes in rats
Authors: Wiczling P, Krzyzanski W.
Journal: Exp Hematol (2008): 119
Authors: Wiczling P, Krzyzanski W.
Journal: Exp Hematol (2008): 119
Comparison of sample fixation and the use of LDS-751 or anti-CD45 for leukocyte identification in mouse whole blood for flow cytometry
Authors: Maes ML, Davidson LB, McDonagh PF, Ritter LS.
Journal: J Immunol Methods (2007): 79
Authors: Maes ML, Davidson LB, McDonagh PF, Ritter LS.
Journal: J Immunol Methods (2007): 79