Lissamine Rhodamine B Sulfonyl Chloride [Sulforhodamine B sulfonyl chloride] CAS 62796-29-6

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Physical properties

Molecular weight	577.11
Solvent	DMF

Spectral properties

Correction Factor (260 nm)	0.21
Correction Factor (280 nm)	0.171
Excitation (nm)	558
Emission (nm)	575

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

62796-29-6

Molecular weight

577.11

Correction Factor (260 nm)

0.21

Correction Factor (280 nm)

0.171

Excitation (nm)

558

Emission (nm)

575

SRB sulfonyl chloride (also called Lissamine rhodamine sulfonyl chloride™) is highly fluorescent. It is quite unstable in water, especially at the higher pH required for reaction with aliphatic amines. Protein modification by this reagent is frequently done at low temperature (preferably at 4 °C). This reagent reacts with amine compounds such as amino acids, peptides and proteins to give bright red fluorescent conjugates that are extremely stable, and resistant to protease-catalyzed hydrolysis. Unlike succinimidyl esters, sulfonyl chlorides react with both aliphatic amines and aromatic amines indiscriminatingly.

Calculators

Common stock solution preparation

Table 1. Volume of DMF needed to reconstitute specific mass of Lissamine Rhodamine B Sulfonyl Chloride [Sulforhodamine B sulfonyl chloride] *CAS 62796-29-6* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	173.277 µL	866.386 µL	1.733 mL	8.664 mL	17.328 mL
5 mM	34.655 µL	173.277 µL	346.554 µL	1.733 mL	3.466 mL
10 mM	17.328 µL	86.639 µL	173.277 µL	866.386 µL	1.733 mL

Molarity calculator

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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.21
Correction Factor (280 nm)	0.171
Excitation (nm)	558
Emission (nm)	575

Images

Figure 1. Chemical structure for Lissamine Rhodamine B Sulfonyl Chloride [Sulforhodamine B sulfonyl chloride] *CAS 62796-29-6*

Citations

View all 1 citations: Citation Explorer

Entry of 6-Residue Antimicrobial Peptide Derived from Lactoferricin B into Single Vesicles and E. coli Cells without Damaging their Membranes
Authors: Moniruzzaman, Md and Islam, Md Zahidul and Sharmin, Sabrina and Dohra, Hideo and Yamazaki, Masahito
Journal: Biochemistry (2017)

References

View all 22 references: Citation Explorer

One-pot fluorescent labeling of xyloglucan oligosaccharides with sulforhodamine
Authors: Kosik O, Farkas V.
Journal: Anal Biochem (2008): 232

O-oligosaccharidyl-1-amino-1-deoxyalditols as intermediates for fluorescent labelling of oligosaccharides
Authors: Miller JG, Farkas V, Sharples SC, Fry SC.
Journal: Carbohydr Res (2007): 44

A novel conjugable translocator protein ligand labeled with a fluorescence dye for in vitro imaging
Authors: Bai M, Wyatt SK, Han Z, Papadopoulos V, Bornhop DJ.
Journal: Bioconjug Chem (2007): 1118

Psoralen conjugates for visualization of genomic interstrand cross-links localized by laser photoactivation
Authors: Thazhathveetil AK, Liu ST, Indig FE, Seidman MM.
Journal: Bioconjug Chem (2007): 431

De novo identification of highly active fluorescent kappa opioid ligands from a rhodamine labeled tetrapeptide positional scanning library
Authors: Houghten RA, Dooley CT, Appel JR.
Journal: Bioorg Med Chem Lett (2004): 1947

Fluorescent probes for monitoring virus fusion kinetics: comparative evaluation of reliability
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Journal: Biochim Biophys Acta (2002): 65

Chemistry of sulforhodamine--amine conjugates
Authors: Corrie JE, Davis CT, Eccleston JF.
Journal: Bioconjug Chem (2001): 186

Increased liposome extravasation in selected tissues: effect of substance P
Authors: Rosenecker J, Zhang W, Hong K, Lausier J, Geppetti P, Yoshihara S, Papahadjopoulos D, Nadel JA.
Journal: Proc Natl Acad Sci U S A (1996): 7236

Texas Res-X and rhodamine Red-X, new derivatives of sulforhodamine 101 and lissamine rhodamine B with improved labeling and fluorescence properties
Authors: Lefevre C, Kang HC, Haugl and RP, Malekzadeh N, Arttamangkul S.
Journal: Bioconjug Chem (1996): 482

A comparison of direct and liposomal antibody conjugates of sulfonated aluminum phthalocyanines for selective photoimmunotherapy of human bladder carcinoma
Authors: Morgan J, Lottman H, Abbou CC, Chopin DK.
Journal: Photochem Photobiol (1994): 486