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Lissamine Rhodamine B Sulfonyl Chloride [Sulforhodamine B sulfonyl chloride] *CAS 62796-29-6*
SRB sulfonyl chloride (also called Lissamine rhodamine sulfonyl chloride™) is highly fluorescent. It is quite unstable in water, especially at the higher pH required for reaction with aliphatic amines. Protein modification by this reagent is frequently done at low temperature (preferably at 4 °C). This reagent reacts with amine compounds such as amino acids, peptides and proteins to give bright red fluorescent conjugates that are extremely stable, and resistant to protease-catalyzed hydrolysis. Unlike succinimidyl esters, sulfonyl chlorides react with both aliphatic amines and aromatic amines indiscriminatingly.
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Common stock solution preparation
Table 1. Volume of DMF needed to reconstitute specific mass of Lissamine Rhodamine B Sulfonyl Chloride [Sulforhodamine B sulfonyl chloride] *CAS 62796-29-6* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 173.277 µL | 866.386 µL | 1.733 mL | 8.664 mL | 17.328 mL |
| 5 mM | 34.655 µL | 173.277 µL | 346.554 µL | 1.733 mL | 3.466 mL |
| 10 mM | 17.328 µL | 86.639 µL | 173.277 µL | 866.386 µL | 1.733 mL |
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Citations
View all 2 citations: Citation Explorer
Relationship between oligoarginine-induced membrane damage of single Escherichia coli cells and entry of the peptide into the cytoplasm
Authors: Sharmin, Sabrina and Islam, Md Zahidul and Yamazaki, Masahito
Journal: Biochemistry and Biophysics Reports (2024): 101777
Authors: Sharmin, Sabrina and Islam, Md Zahidul and Yamazaki, Masahito
Journal: Biochemistry and Biophysics Reports (2024): 101777
Entry of 6-Residue Antimicrobial Peptide Derived from Lactoferricin B into Single Vesicles and E. coli Cells without Damaging their Membranes
Authors: Moniruzzaman, Md and Islam, Md Zahidul and Sharmin, Sabrina and Dohra, Hideo and Yamazaki, Masahito
Journal: Biochemistry (2017)
Authors: Moniruzzaman, Md and Islam, Md Zahidul and Sharmin, Sabrina and Dohra, Hideo and Yamazaki, Masahito
Journal: Biochemistry (2017)
References
View all 22 references: Citation Explorer
One-pot fluorescent labeling of xyloglucan oligosaccharides with sulforhodamine
Authors: Kosik O, Farkas V.
Journal: Anal Biochem (2008): 232
Authors: Kosik O, Farkas V.
Journal: Anal Biochem (2008): 232
O-oligosaccharidyl-1-amino-1-deoxyalditols as intermediates for fluorescent labelling of oligosaccharides
Authors: Miller JG, Farkas V, Sharples SC, Fry SC.
Journal: Carbohydr Res (2007): 44
Authors: Miller JG, Farkas V, Sharples SC, Fry SC.
Journal: Carbohydr Res (2007): 44
A novel conjugable translocator protein ligand labeled with a fluorescence dye for in vitro imaging
Authors: Bai M, Wyatt SK, Han Z, Papadopoulos V, Bornhop DJ.
Journal: Bioconjug Chem (2007): 1118
Authors: Bai M, Wyatt SK, Han Z, Papadopoulos V, Bornhop DJ.
Journal: Bioconjug Chem (2007): 1118
Psoralen conjugates for visualization of genomic interstrand cross-links localized by laser photoactivation
Authors: Thazhathveetil AK, Liu ST, Indig FE, Seidman MM.
Journal: Bioconjug Chem (2007): 431
Authors: Thazhathveetil AK, Liu ST, Indig FE, Seidman MM.
Journal: Bioconjug Chem (2007): 431
De novo identification of highly active fluorescent kappa opioid ligands from a rhodamine labeled tetrapeptide positional scanning library
Authors: Houghten RA, Dooley CT, Appel JR.
Journal: Bioorg Med Chem Lett (2004): 1947
Authors: Houghten RA, Dooley CT, Appel JR.
Journal: Bioorg Med Chem Lett (2004): 1947
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