Name: LiveONLY™ Nuclear Green
Brand: AAT Bioquest
SKU: 17687
Price: 301 USD
Availability: InStock

LiveONLY™ Nuclear Green

LiveONLY™ Nuclear Green™ is a cell-permeable nuclear stain that exclusively labels live cell nuclei while excluding dead or membrane-compromised cells, enabling accurate viability assessment in heterogeneous populations.

First-in-class: First fluorescent dye designed for nuclear staining exclusively in live cells.
Selective Live Nuclear Staining: Specifically stains nuclei in live cells without background signal from cytoplasm or dead cells
Live Cell Compatible: Membrane-permeable allowing for real-time imaging of live cells
No Wash Formula: Simple mix-and-read protocol, eliminates the need for washes
Bright and Versatile: Bright green signal, compatible with a variety of imaging systems

The fluorescence images of HeLa cells stained with LiveONLY™ Nuclear Green (#17687) and Cytofix Red Mitochondrial Stain (#23200). The LiveONLY™ Nuclear Green (#17687) is showing selective nuclear staining in live cells only; dead cells do not show any nuclear staining.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17687	200 tests	Price

Overview

LiveONLY™ Nuclear Green™ is a nuclear dye designed to stain nucleus exclusively in live cells. This unique feature allows to distinguish live cells from dead or membrane-compromised cells. Its cell permeable design enables selective accumulation in the nucleus, producing a green fluorescence without the need for fixation or permeabilization.

The simple mix-and-read protocol requires minimal hands-on time, making it ideal for seamless integration into standard imaging workflows. Compatible with a wide range of cell lines and imaging systems, LiveONLY™ Nuclear Green™ is ideal for live-cell applications such as real-time cell tracking, viability assessment, and high-content screening.

References

View all 50 references: Citation Explorer

4D Microscopy and Tracking of Chromosomes and the Spindle in C. elegans Early Embryos.

Authors:

Dumont, Julien and Maton, Gilliane

Journal:

Methods in molecular biology (Clifton, N.J.) (2025): 141-156

Virtual staining from bright-field microscopy for label-free quantitative analysis of plant cell structures.

Authors:

Ichita, Manami and Yamamichi, Haruna and Higaki, Takumi

Journal:

Plant molecular biology (2025): 29

Super-Resolution Imaging of Mitotic Spindle Microtubules Using STED Microscopy.

Authors:

Koprivec, Isabella and Štimac, Valentina and Tolić, Iva M

Journal:

Methods in molecular biology (Clifton, N.J.) (2025): 3-19

Live-cell super-resolution microscopy reveals how molecules enter and exit the nucleus.

Authors:

Rieger, Bernd and Berrevoets, Enya S

Journal:

Nature (2025): 607-608

The number of nuclei in compacted embryos, assessed by optical coherence microscopy, is a non-invasive and robust marker of mouse embryo quality.

Authors:

Sobkowiak, Aleksandra and Fluks, Monika and Kosyl, Ewa and Milewski, Robert and Szpila, Marcin and Tamborski, Szymon and Szkulmowski, Maciej and Ajduk, Anna

Journal:

Molecular human reproduction (2024)

Physical properties

Solvent

DMSO

Spectral properties

Excitation (nm)	503
Emission (nm)	527

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Fluorescence microscope
Excitation	FITC filter
Emission	FITC filter
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on August 25, 2026