LRB Red™ SE

Although Lissamine Rhodamine B chloride is one of the most popular labeling reagents of sulfonyl chloride, it is quite unstable in water. Even in anhydrous DMF, Lissamine Rhodamine B chloride tends to give a very complicated reaction mixture. It reacts with thiols, alcohols, phenols, aliphatic amines and aromatic amines indiscriminatingly. LRB Red™ is a succinimidyl ester and has the spectral properties identical to those of Lissamine Rhodamine B chloride. LRB Red™ is a superior replacement for Lissamine Rhodamine B chloride. LRB Red™ only reacts with aliphatic amines such as amino acids, peptides and proteins to give bright red fluorescent conjugates that are extremely stable. Compared to Lissamine Rhodamine B chloride, LRB Red™ has much higher labeling efficiency, and more importantly the resulted conjugates are much easier to be purified due to its much cleaner reactions. We strongly recommend that you consider using LRB Red™ to replace Lissamine Rhodamine B chloride for labeling peptides and oligonucleotides.

Protocol

SDS

COO

COA

Catalog	Size	Price	Quantity
471	10 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of LRB Red™ SE to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	130.398 µL	651.992 µL	1.304 mL	6.52 mL	13.04 mL
5 mM	26.08 µL	130.398 µL	260.797 µL	1.304 mL	2.608 mL
10 mM	13.04 µL	65.199 µL	130.398 µL	651.992 µL	1.304 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	766.88 g/mol	=	mL	x	mM	=	mmol

Citations

View all 2 citations: Citation Explorer

Entry of a six-residue antimicrobial peptide derived from lactoferricin B into single vesicles and Escherichia coli cells without damaging their membranes

Authors:

Moniruzzaman, Md and Islam, Md Zahidul and Sharmin, Sabrina and Dohra, Hideo and Yamazaki, Masahito

Journal:

Biochemistry (2017): 4419--4431

Entry of 6-Residue Antimicrobial Peptide Derived from Lactoferricin B into Single Vesicles and E. coli Cells without Damaging their Membranes

Authors:

Moniruzzaman, Md and Islam, Md Zahidul and Sharmin, Sabrina and Dohra, Hideo and Yamazaki, Masahito

Journal:

Biochemistry (2017)

References

View all 22 references: Citation Explorer

One-pot fluorescent labeling of xyloglucan oligosaccharides with sulforhodamine

Authors:

Kosik O, Farkas V.

Journal:

Anal Biochem (2008): 232

O-oligosaccharidyl-1-amino-1-deoxyalditols as intermediates for fluorescent labelling of oligosaccharides

Authors:

Miller JG, Farkas V, Sharples SC, Fry SC.

Journal:

Carbohydr Res (2007): 44

A novel conjugable translocator protein ligand labeled with a fluorescence dye for in vitro imaging

Authors:

Bai M, Wyatt SK, Han Z, Papadopoulos V, Bornhop DJ.

Journal:

Bioconjug Chem (2007): 1118

Psoralen conjugates for visualization of genomic interstrand cross-links localized by laser photoactivation

Authors:

Thazhathveetil AK, Liu ST, Indig FE, Seidman MM.

Journal:

Bioconjug Chem (2007): 431

De novo identification of highly active fluorescent kappa opioid ligands from a rhodamine labeled tetrapeptide positional scanning library

Authors:

Houghten RA, Dooley CT, Appel JR.

Journal:

Bioorg Med Chem Lett (2004): 1947

Physical properties

Molecular weight	766.88
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.21
Correction factor (280 nm)	0.171
Excitation (nm)	558
Emission (nm)	575

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 12, 2025