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LRB Red™ SE

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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Physical properties
Molecular weight766.88
SolventDMSO
Spectral properties
Correction Factor (260 nm)0.21
Correction Factor (280 nm)0.171
Excitation (nm)558
Emission (nm)575
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


Molecular weight
766.88
Correction Factor (260 nm)
0.21
Correction Factor (280 nm)
0.171
Excitation (nm)
558
Emission (nm)
575
Although Lissamine Rhodamine B chloride is one of the most popular labeling reagents of sulfonyl chloride, it is quite unstable in water. Even in anhydrous DMF, Lissamine Rhodamine B chloride tends to give a very complicated reaction mixture. It reacts with thiols, alcohols, phenols, aliphatic amines and aromatic amines indiscriminatingly. LRB Red™ is a succinimidyl ester and has the spectral properties identical to those of Lissamine Rhodamine B chloride. LRB Red™ is a superior replacement for Lissamine Rhodamine B chloride. LRB Red™ only reacts with aliphatic amines such as amino acids, peptides and proteins to give bright red fluorescent conjugates that are extremely stable. Compared to Lissamine Rhodamine B chloride, LRB Red™ has much higher labeling efficiency, and more importantly the resulted conjugates are much easier to be purified due to its much cleaner reactions. We strongly recommend that you consider using LRB Red™ to replace Lissamine Rhodamine B chloride for labeling peptides and oligonucleotides.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of LRB Red™ SE to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM130.398 µL651.992 µL1.304 mL6.52 mL13.04 mL
5 mM26.08 µL130.398 µL260.797 µL1.304 mL2.608 mL
10 mM13.04 µL65.199 µL130.398 µL651.992 µL1.304 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Correction Factor (260 nm)0.21
Correction Factor (280 nm)0.171
Excitation (nm)558
Emission (nm)575

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
Sunnyvale Red™ SE *Superior 6-ROX Replacement*591624---
California Red™ SE59260910000010.240.194

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Citations


View all 2 citations: Citation Explorer
Entry of a six-residue antimicrobial peptide derived from lactoferricin B into single vesicles and Escherichia coli cells without damaging their membranes
Authors: Moniruzzaman, Md and Islam, Md Zahidul and Sharmin, Sabrina and Dohra, Hideo and Yamazaki, Masahito
Journal: Biochemistry (2017): 4419--4431
Entry of 6-Residue Antimicrobial Peptide Derived from Lactoferricin B into Single Vesicles and E. coli Cells without Damaging their Membranes
Authors: Moniruzzaman, Md and Islam, Md Zahidul and Sharmin, Sabrina and Dohra, Hideo and Yamazaki, Masahito
Journal: Biochemistry (2017)

References


View all 22 references: Citation Explorer
One-pot fluorescent labeling of xyloglucan oligosaccharides with sulforhodamine
Authors: Kosik O, Farkas V.
Journal: Anal Biochem (2008): 232
O-oligosaccharidyl-1-amino-1-deoxyalditols as intermediates for fluorescent labelling of oligosaccharides
Authors: Miller JG, Farkas V, Sharples SC, Fry SC.
Journal: Carbohydr Res (2007): 44
A novel conjugable translocator protein ligand labeled with a fluorescence dye for in vitro imaging
Authors: Bai M, Wyatt SK, Han Z, Papadopoulos V, Bornhop DJ.
Journal: Bioconjug Chem (2007): 1118
Psoralen conjugates for visualization of genomic interstrand cross-links localized by laser photoactivation
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Journal: Bioconjug Chem (2007): 431
De novo identification of highly active fluorescent kappa opioid ligands from a rhodamine labeled tetrapeptide positional scanning library
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Fluorescent probes for monitoring virus fusion kinetics: comparative evaluation of reliability
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Journal: Biochim Biophys Acta (2002): 65
Chemistry of sulforhodamine--amine conjugates
Authors: Corrie JE, Davis CT, Eccleston JF.
Journal: Bioconjug Chem (2001): 186
Increased liposome extravasation in selected tissues: effect of substance P
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Journal: Proc Natl Acad Sci U S A (1996): 7236
Texas Res-X and rhodamine Red-X, new derivatives of sulforhodamine 101 and lissamine rhodamine B with improved labeling and fluorescence properties
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Journal: Bioconjug Chem (1996): 482
A comparison of direct and liposomal antibody conjugates of sulfonated aluminum phthalocyanines for selective photoimmunotherapy of human bladder carcinoma
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