AAT Bioquest


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InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight766.88
Spectral properties
Correction Factor (260 nm)0.21
Correction Factor (280 nm)0.171
Excitation (nm)558
Emission (nm)575
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Correction Factor (260 nm)
Correction Factor (280 nm)
Excitation (nm)
Emission (nm)
Although Lissamine Rhodamine B chloride is one of the most popular labeling reagents of sulfonyl chloride, it is quite unstable in water. Even in anhydrous DMF, Lissamine Rhodamine B chloride tends to give a very complicated reaction mixture. It reacts with thiols, alcohols, phenols, aliphatic amines and aromatic amines indiscriminatingly. LRB Red™ is a succinimidyl ester and has the spectral properties identical to those of Lissamine Rhodamine B chloride. LRB Red™ is a superior replacement for Lissamine Rhodamine B chloride. LRB Red™ only reacts with aliphatic amines such as amino acids, peptides and proteins to give bright red fluorescent conjugates that are extremely stable. Compared to Lissamine Rhodamine B chloride, LRB Red™ has much higher labeling efficiency, and more importantly the resulted conjugates are much easier to be purified due to its much cleaner reactions. We strongly recommend that you consider using LRB Red™ to replace Lissamine Rhodamine B chloride for labeling peptides and oligonucleotides.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of LRB Red™ SE to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM130.398 µL651.992 µL1.304 mL6.52 mL13.04 mL
5 mM26.08 µL130.398 µL260.797 µL1.304 mL2.608 mL
10 mM13.04 µL65.199 µL130.398 µL651.992 µL1.304 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Spectral properties

Correction Factor (260 nm)0.21
Correction Factor (280 nm)0.171
Excitation (nm)558
Emission (nm)575

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
Sunnyvale Red™ SE *Superior 6-ROX Replacement*591624---
California Red™ SE59260910000010.240.194



View all 2 citations: Citation Explorer
Entry of a six-residue antimicrobial peptide derived from lactoferricin B into single vesicles and Escherichia coli cells without damaging their membranes
Authors: Moniruzzaman, Md and Islam, Md Zahidul and Sharmin, Sabrina and Dohra, Hideo and Yamazaki, Masahito
Journal: Biochemistry (2017): 4419--4431
Entry of 6-Residue Antimicrobial Peptide Derived from Lactoferricin B into Single Vesicles and E. coli Cells without Damaging their Membranes
Authors: Moniruzzaman, Md and Islam, Md Zahidul and Sharmin, Sabrina and Dohra, Hideo and Yamazaki, Masahito
Journal: Biochemistry (2017)


View all 22 references: Citation Explorer
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O-oligosaccharidyl-1-amino-1-deoxyalditols as intermediates for fluorescent labelling of oligosaccharides
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A novel conjugable translocator protein ligand labeled with a fluorescence dye for in vitro imaging
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