LRB Red™ SE

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	766.88
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.21
Correction Factor (280 nm)	0.171
Excitation (nm)	558
Emission (nm)	575

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

766.88

Correction Factor (260 nm)

0.21

Correction Factor (280 nm)

0.171

Excitation (nm)

558

Emission (nm)

575

Although Lissamine Rhodamine B chloride is one of the most popular labeling reagents of sulfonyl chloride, it is quite unstable in water. Even in anhydrous DMF, Lissamine Rhodamine B chloride tends to give a very complicated reaction mixture. It reacts with thiols, alcohols, phenols, aliphatic amines and aromatic amines indiscriminatingly. LRB Red™ is a succinimidyl ester and has the spectral properties identical to those of Lissamine Rhodamine B chloride. LRB Red™ is a superior replacement for Lissamine Rhodamine B chloride. LRB Red™ only reacts with aliphatic amines such as amino acids, peptides and proteins to give bright red fluorescent conjugates that are extremely stable. Compared to Lissamine Rhodamine B chloride, LRB Red™ has much higher labeling efficiency, and more importantly the resulted conjugates are much easier to be purified due to its much cleaner reactions. We strongly recommend that you consider using LRB Red™ to replace Lissamine Rhodamine B chloride for labeling peptides and oligonucleotides.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of LRB Red™ SE to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	130.398 µL	651.992 µL	1.304 mL	6.52 mL	13.04 mL
5 mM	26.08 µL	130.398 µL	260.797 µL	1.304 mL	2.608 mL
10 mM	13.04 µL	65.199 µL	130.398 µL	651.992 µL	1.304 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (260 nm)	0.21
Correction Factor (280 nm)	0.171
Excitation (nm)	558
Emission (nm)	575

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (260 nm)	Correction Factor (280 nm)
Sunnyvale Red™ SE Superior 6-ROX Replacement	591	624	-	-	-
California Red™ SE	592	609	100000¹	0.24	0.194

Images

Figure 1. Chemical structure for LRB Red™ SE

Citations

View all 2 citations: Citation Explorer

Entry of a six-residue antimicrobial peptide derived from lactoferricin B into single vesicles and Escherichia coli cells without damaging their membranes
Authors: Moniruzzaman, Md and Islam, Md Zahidul and Sharmin, Sabrina and Dohra, Hideo and Yamazaki, Masahito
Journal: Biochemistry (2017): 4419--4431

Entry of 6-Residue Antimicrobial Peptide Derived from Lactoferricin B into Single Vesicles and E. coli Cells without Damaging their Membranes
Authors: Moniruzzaman, Md and Islam, Md Zahidul and Sharmin, Sabrina and Dohra, Hideo and Yamazaki, Masahito
Journal: Biochemistry (2017)

References

View all 22 references: Citation Explorer

One-pot fluorescent labeling of xyloglucan oligosaccharides with sulforhodamine
Authors: Kosik O, Farkas V.
Journal: Anal Biochem (2008): 232

O-oligosaccharidyl-1-amino-1-deoxyalditols as intermediates for fluorescent labelling of oligosaccharides
Authors: Miller JG, Farkas V, Sharples SC, Fry SC.
Journal: Carbohydr Res (2007): 44

A novel conjugable translocator protein ligand labeled with a fluorescence dye for in vitro imaging
Authors: Bai M, Wyatt SK, Han Z, Papadopoulos V, Bornhop DJ.
Journal: Bioconjug Chem (2007): 1118

Psoralen conjugates for visualization of genomic interstrand cross-links localized by laser photoactivation
Authors: Thazhathveetil AK, Liu ST, Indig FE, Seidman MM.
Journal: Bioconjug Chem (2007): 431

De novo identification of highly active fluorescent kappa opioid ligands from a rhodamine labeled tetrapeptide positional scanning library
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Journal: Bioorg Med Chem Lett (2004): 1947

Fluorescent probes for monitoring virus fusion kinetics: comparative evaluation of reliability
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Journal: Biochim Biophys Acta (2002): 65

Chemistry of sulforhodamine--amine conjugates
Authors: Corrie JE, Davis CT, Eccleston JF.
Journal: Bioconjug Chem (2001): 186

Increased liposome extravasation in selected tissues: effect of substance P
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Journal: Proc Natl Acad Sci U S A (1996): 7236

Texas Res-X and rhodamine Red-X, new derivatives of sulforhodamine 101 and lissamine rhodamine B with improved labeling and fluorescence properties
Authors: Lefevre C, Kang HC, Haugl and RP, Malekzadeh N, Arttamangkul S.
Journal: Bioconjug Chem (1996): 482

A comparison of direct and liposomal antibody conjugates of sulfonated aluminum phthalocyanines for selective photoimmunotherapy of human bladder carcinoma
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Journal: Photochem Photobiol (1994): 486