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Luciferase *Recombinant firefly*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | ~60 kDa |
| Solvent | Water |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 41105331 |
| Overview |  SDSProtocol |
See also: Luciferases
CAS 61970-00-1 | Molecular weight ~60 kDa |
This enzyme is a luciferase expressed from a cloned gene from the North American firefly (Photinus pyralis) that provides the reliability and dependability needed for performing research or producing kits using bioluminescence reagents to detect ATP or luciferin substrates. This recombinant enzyme can potentially eliminate the possibility of seasonal and regional variability that may be found in luciferase purified from natural sources.
Images
Figure 1. Luciferase activity was measured with Amplite® Luciferase Reporter Gene Assay Kit in a white 96-well plate with a NOVOstar plate reader (BMG Labtech). The luciferase activity is still detectable at 0.1 pg/well with 20 minutes incubation.
Figure 2. Comparison of the heat stability of Luciferase *Recombinant firefly* and Luciferase(from Vendor A) after incubation at 60 °C.
References
View all 40 references: Citation Explorer
Measuring HIV Neutralization in a Luciferase Reporter Gene Assay
Authors: Montefiori DC., undefined
Journal: Methods Mol Biol (2009): 395
Authors: Montefiori DC., undefined
Journal: Methods Mol Biol (2009): 395
An optimized luciferase bioluminescent assay for coenzyme A
Authors: Marques SM, da Silva JC.
Journal: Anal Bioanal Chem (2008): 2161
Authors: Marques SM, da Silva JC.
Journal: Anal Bioanal Chem (2008): 2161
Quantitative assessment of the knockdown efficiency of morpholino antisense oligonucleotides in zebrafish embryos using a luciferase assay
Authors: Kamachi Y, Okuda Y, Kondoh H.
Journal: Genesis (2008): 1
Authors: Kamachi Y, Okuda Y, Kondoh H.
Journal: Genesis (2008): 1
A cell-based luciferase assay amenable to high-throughput screening of inhibitors of arenavirus budding
Authors: Capul AA, de la Torre JC.
Journal: Virology (2008): 107
Authors: Capul AA, de la Torre JC.
Journal: Virology (2008): 107
Application of the dual-luciferase reporter assay to the analysis of promoter activity in Zebrafish embryos
Authors: Alcaraz-Perez F, Mulero V, Cayuela ML.
Journal: BMC Biotechnol (2008): 81
Authors: Alcaraz-Perez F, Mulero V, Cayuela ML.
Journal: BMC Biotechnol (2008): 81
Quantitation of autophagy by luciferase release assay
Authors: Ketteler R, Seed B.
Journal: Autophagy (2008): 801
Authors: Ketteler R, Seed B.
Journal: Autophagy (2008): 801
Firefly luciferase complementation imaging assay for protein-protein interactions in plants
Authors: Chen H, Zou Y, Shang Y, Lin H, Wang Y, Cai R, Tang X, Zhou JM.
Journal: Plant Physiol (2008): 368
Authors: Chen H, Zou Y, Shang Y, Lin H, Wang Y, Cai R, Tang X, Zhou JM.
Journal: Plant Physiol (2008): 368
Determination of silencing potency of synthetic and RNase III-generated siRNA using a secreted luciferase assay
Authors: Morlighem JE, Petit C, Tzertzinis G.
Journal: Biotechniques (2007): 599
Authors: Morlighem JE, Petit C, Tzertzinis G.
Journal: Biotechniques (2007): 599
A luciferase-based budding assay for Ebola virus
Authors: McCarthy SE, Licata JM, Harty RN.
Journal: J Virol Methods (2006): 115
Authors: McCarthy SE, Licata JM, Harty RN.
Journal: J Virol Methods (2006): 115
Luciferase reporter assay
Authors: Wettey FR, Jackson AP.
Journal: Subcell Biochem (2006): 423
Authors: Wettey FR, Jackson AP.
Journal: Subcell Biochem (2006): 423
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Selective Detection of Pyrophosphate Using a Fluorogenic Pyrophosphate Sensor
Restriction of Advanced Glycation End Products Improves Insulin Resistance in Human Type 2 Diabetes
Matrix Remodeling Maintains Embryonic Stem Cell Self-Renewal by Activating Stat3
Human High Temperature Requirement Serine Protease A1 (HTRA1) Degrades Tau Protein Aggregates
Selective Detection of Pyrophosphate Using a Fluorogenic Pyrophosphate Sensor
Restriction of Advanced Glycation End Products Improves Insulin Resistance in Human Type 2 Diabetes
Matrix Remodeling Maintains Embryonic Stem Cell Self-Renewal by Activating Stat3
Human High Temperature Requirement Serine Protease A1 (HTRA1) Degrades Tau Protein Aggregates