Lucigenin [Bis-N-methylacridinium nitrate]

Lucigenin

Bis-N-methylacridinium nitrate

A chloride indicator that has the fluorescence dependent of chloride concentration. Its fluorescence is quenched by chloride through collision quenching. In addition, it is also used for detecting superoxide.

Catalog	Size	Price	Quantity
21259	10 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Lucigenin [Bis-N-methylacridinium nitrate] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	195.886 µL	979.432 µL	1.959 mL	9.794 mL	19.589 mL
5 mM	39.177 µL	195.886 µL	391.773 µL	1.959 mL	3.918 mL
10 mM	19.589 µL	97.943 µL	195.886 µL	979.432 µL	1.959 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	510.5 g/mol	=	mL	x	mM	=	mmol

References

View all 33 references: Citation Explorer

Flow injection chemiluminescence determination of isoniazid using the lucigenin-periodate system

Authors:

Du J, Lu J.

Journal:

Luminescence (2006): 26

Identification of cytochrome-b5 reductase as the enzyme responsible for NADH-dependent lucigenin chemiluminescence in human spermatozoa

Authors:

Baker MA, Krutskikh A, Curry BJ, Hetherington L, Aitken RJ.

Journal:

Biol Reprod (2005): 334

Chemiluminescence of lucigenin is dependent on experimental conditions

Authors:

Hyrsl P, Lojek A, Ciz M, Kubala L.

Journal:

Luminescence (2004): 61

Luminol-, isoluminol- and lucigenin-enhanced chemiluminescence of rat blood phagocytes stimulated with different activators

Authors:

Pavelkova M, Kubala L.

Journal:

Luminescence (2004): 37

Lucigenin and coelenterazine as superoxide probes in mitochondrial and bacterial membranes

Authors:

Kervinen M, Patsi J, Finel M, Hassinen IE.

Journal:

Anal Biochem (2004): 45

Physical properties

Molecular weight	510.5
Solvent	DMSO

Spectral properties

Excitation (nm)	369
Emission (nm)	503

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	22103-92-0

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Page updated on October 16, 2025