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Lucigenin [Bis-N-methylacridinium nitrate]

A chloride indicator that has the fluorescence dependent of chloride concentration. Its fluorescence is quenched by chloride through collision quenching. In addition, it is also used for detecting superoxide.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Lucigenin [Bis-N-methylacridinium nitrate] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM195.886 µL979.432 µL1.959 mL9.794 mL19.589 mL
5 mM39.177 µL195.886 µL391.773 µL1.959 mL3.918 mL
10 mM19.589 µL97.943 µL195.886 µL979.432 µL1.959 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

References

View all 33 references: Citation Explorer
Flow injection chemiluminescence determination of isoniazid using the lucigenin-periodate system
Authors: Du J, Lu J.
Journal: Luminescence (2006): 26
Identification of cytochrome-b5 reductase as the enzyme responsible for NADH-dependent lucigenin chemiluminescence in human spermatozoa
Authors: Baker MA, Krutskikh A, Curry BJ, Hetherington L, Aitken RJ.
Journal: Biol Reprod (2005): 334
Chemiluminescence of lucigenin is dependent on experimental conditions
Authors: Hyrsl P, Lojek A, Ciz M, Kubala L.
Journal: Luminescence (2004): 61
Luminol-, isoluminol- and lucigenin-enhanced chemiluminescence of rat blood phagocytes stimulated with different activators
Authors: Pavelkova M, Kubala L.
Journal: Luminescence (2004): 37
Lucigenin and coelenterazine as superoxide probes in mitochondrial and bacterial membranes
Authors: Kervinen M, Patsi J, Finel M, Hassinen IE.
Journal: Anal Biochem (2004): 45
Page updated on October 12, 2024

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Physical properties

Molecular weight

510.5

Solvent

DMSO

Spectral properties

Excitation (nm)

369

Emission (nm)

503

Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200

CAS

22103-92-0
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