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Lucigenin [Bis-N-methylacridinium nitrate]

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Physical properties
Molecular weight510.5
Spectral properties
Excitation (nm)369
Emission (nm)503
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
A chloride indicator that has the fluorescence dependent of chloride concentration. Its fluorescence is quenched by chloride through collision quenching. In addition, it is also used for detecting superoxide.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Lucigenin [Bis-N-methylacridinium nitrate] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM195.886 µL979.432 µL1.959 mL9.794 mL19.589 mL
5 mM39.177 µL195.886 µL391.773 µL1.959 mL3.918 mL
10 mM19.589 µL97.943 µL195.886 µL979.432 µL1.959 mL

Molarity calculator

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Spectral properties

Excitation (nm)369
Emission (nm)503



View all 33 references: Citation Explorer
Flow injection chemiluminescence determination of isoniazid using the lucigenin-periodate system
Authors: Du J, Lu J.
Journal: Luminescence (2006): 26
Identification of cytochrome-b5 reductase as the enzyme responsible for NADH-dependent lucigenin chemiluminescence in human spermatozoa
Authors: Baker MA, Krutskikh A, Curry BJ, Hetherington L, Aitken RJ.
Journal: Biol Reprod (2005): 334
Chemiluminescence of lucigenin is dependent on experimental conditions
Authors: Hyrsl P, Lojek A, Ciz M, Kubala L.
Journal: Luminescence (2004): 61
Luminol-, isoluminol- and lucigenin-enhanced chemiluminescence of rat blood phagocytes stimulated with different activators
Authors: Pavelkova M, Kubala L.
Journal: Luminescence (2004): 37
Lucigenin and coelenterazine as superoxide probes in mitochondrial and bacterial membranes
Authors: Kervinen M, Patsi J, Finel M, Hassinen IE.
Journal: Anal Biochem (2004): 45
Identification of cytochrome P450-reductase as the enzyme responsible for NADPH-dependent lucigenin and tetrazolium salt reduction in rat epididymal sperm preparations
Authors: Baker MA, Krutskikh A, Curry BJ, McLaughlin EA, Aitken RJ.
Journal: Biol Reprod (2004): 307
Electrogenerated chemiluminescence reaction of lucigenin with isatin at a platinum electrode
Authors: Qi H, Zhang C.
Journal: Luminescence (2004): 21
Chemiluminescent assay for biological substances--chemiluminescent assay for alkaline phosphatase using lucigenin and its application to enzyme immunoassay
Authors: Maeda M., undefined
Journal: Rinsho Byori (2004): 851
Chemiluminescence of lucigenin by electrogenerated superoxide ions in aqueous solutions
Authors: Okajima T, Ohsaka T.
Journal: Luminescence (2003): 49
Effect of azole derivatives on lucigenin-dependent microsome chemiluminescence
Authors: Shchepetkin IA, Akhmedzhanov RR, Kagiia VT.
Journal: Biomed Khim (2003): 278