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Lucigenin [Bis-N-methylacridinium nitrate]
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Application notes
A chloride indicator that has the fluorescence dependent of chloride concentration. Its fluorescence is quenched by chloride through collision quenching. In addition, it is also used for detecting superoxide.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Lucigenin [Bis-N-methylacridinium nitrate] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 195.886 µL | 979.432 µL | 1.959 mL | 9.794 mL | 19.589 mL |
| 5 mM | 39.177 µL | 195.886 µL | 391.773 µL | 1.959 mL | 3.918 mL |
| 10 mM | 19.589 µL | 97.943 µL | 195.886 µL | 979.432 µL | 1.959 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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References
View all 33 references: Citation Explorer
Flow injection chemiluminescence determination of isoniazid using the lucigenin-periodate system
Authors: Du J, Lu J.
Journal: Luminescence (2006): 26
Authors: Du J, Lu J.
Journal: Luminescence (2006): 26
Identification of cytochrome-b5 reductase as the enzyme responsible for NADH-dependent lucigenin chemiluminescence in human spermatozoa
Authors: Baker MA, Krutskikh A, Curry BJ, Hetherington L, Aitken RJ.
Journal: Biol Reprod (2005): 334
Authors: Baker MA, Krutskikh A, Curry BJ, Hetherington L, Aitken RJ.
Journal: Biol Reprod (2005): 334
Chemiluminescence of lucigenin is dependent on experimental conditions
Authors: Hyrsl P, Lojek A, Ciz M, Kubala L.
Journal: Luminescence (2004): 61
Authors: Hyrsl P, Lojek A, Ciz M, Kubala L.
Journal: Luminescence (2004): 61
Luminol-, isoluminol- and lucigenin-enhanced chemiluminescence of rat blood phagocytes stimulated with different activators
Authors: Pavelkova M, Kubala L.
Journal: Luminescence (2004): 37
Authors: Pavelkova M, Kubala L.
Journal: Luminescence (2004): 37
Lucigenin and coelenterazine as superoxide probes in mitochondrial and bacterial membranes
Authors: Kervinen M, Patsi J, Finel M, Hassinen IE.
Journal: Anal Biochem (2004): 45
Authors: Kervinen M, Patsi J, Finel M, Hassinen IE.
Journal: Anal Biochem (2004): 45