Lucigenin [Bis-N-methylacridinium nitrate]
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
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Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 510.5 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 369 |
Emission (nm) | 503 |
Storage, safety and handling
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | SDSProtocol |
See also: Reactive Oxygen Species (ROS)
CAS 22103-92-0 | Molecular weight 510.5 | Excitation (nm) 369 | Emission (nm) 503 |
A chloride indicator that has the fluorescence dependent of chloride concentration. Its fluorescence is quenched by chloride through collision quenching. In addition, it is also used for detecting superoxide.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Lucigenin [Bis-N-methylacridinium nitrate] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 195.886 µL | 979.432 µL | 1.959 mL | 9.794 mL | 19.589 mL |
5 mM | 39.177 µL | 195.886 µL | 391.773 µL | 1.959 mL | 3.918 mL |
10 mM | 19.589 µL | 97.943 µL | 195.886 µL | 979.432 µL | 1.959 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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References
View all 33 references: Citation Explorer
Flow injection chemiluminescence determination of isoniazid using the lucigenin-periodate system
Authors: Du J, Lu J.
Journal: Luminescence (2006): 26
Authors: Du J, Lu J.
Journal: Luminescence (2006): 26
Identification of cytochrome-b5 reductase as the enzyme responsible for NADH-dependent lucigenin chemiluminescence in human spermatozoa
Authors: Baker MA, Krutskikh A, Curry BJ, Hetherington L, Aitken RJ.
Journal: Biol Reprod (2005): 334
Authors: Baker MA, Krutskikh A, Curry BJ, Hetherington L, Aitken RJ.
Journal: Biol Reprod (2005): 334
Chemiluminescent assay for biological substances--chemiluminescent assay for alkaline phosphatase using lucigenin and its application to enzyme immunoassay
Authors: Maeda M., undefined
Journal: Rinsho Byori (2004): 851
Authors: Maeda M., undefined
Journal: Rinsho Byori (2004): 851
Electrogenerated chemiluminescence reaction of lucigenin with isatin at a platinum electrode
Authors: Qi H, Zhang C.
Journal: Luminescence (2004): 21
Authors: Qi H, Zhang C.
Journal: Luminescence (2004): 21
Identification of cytochrome P450-reductase as the enzyme responsible for NADPH-dependent lucigenin and tetrazolium salt reduction in rat epididymal sperm preparations
Authors: Baker MA, Krutskikh A, Curry BJ, McLaughlin EA, Aitken RJ.
Journal: Biol Reprod (2004): 307
Authors: Baker MA, Krutskikh A, Curry BJ, McLaughlin EA, Aitken RJ.
Journal: Biol Reprod (2004): 307
Lucigenin and coelenterazine as superoxide probes in mitochondrial and bacterial membranes
Authors: Kervinen M, Patsi J, Finel M, Hassinen IE.
Journal: Anal Biochem (2004): 45
Authors: Kervinen M, Patsi J, Finel M, Hassinen IE.
Journal: Anal Biochem (2004): 45
Luminol-, isoluminol- and lucigenin-enhanced chemiluminescence of rat blood phagocytes stimulated with different activators
Authors: Pavelkova M, Kubala L.
Journal: Luminescence (2004): 37
Authors: Pavelkova M, Kubala L.
Journal: Luminescence (2004): 37
Chemiluminescence of lucigenin is dependent on experimental conditions
Authors: Hyrsl P, Lojek A, Ciz M, Kubala L.
Journal: Luminescence (2004): 61
Authors: Hyrsl P, Lojek A, Ciz M, Kubala L.
Journal: Luminescence (2004): 61
Chemiluminescence of lucigenin by electrogenerated superoxide ions in aqueous solutions
Authors: Okajima T, Ohsaka T.
Journal: Luminescence (2003): 49
Authors: Okajima T, Ohsaka T.
Journal: Luminescence (2003): 49
Effect of azole derivatives on lucigenin-dependent microsome chemiluminescence
Authors: Shchepetkin IA, Akhmedzhanov RR, Kagiia VT.
Journal: Biomed Khim (2003): 278
Authors: Shchepetkin IA, Akhmedzhanov RR, Kagiia VT.
Journal: Biomed Khim (2003): 278
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FAQ
Why should I use an absorbance ratio at A575nm/A605nm when using most of your Amplite® Colorimetric Assay Kits?
How should I reconstitute an NADPH standard?
How can I lyse my cells without lysing the nuclear membrane?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
What is the best way to stain cells with Membrane Marker 1-43 (MM 1-43)?
How should I reconstitute an NADPH standard?
How can I lyse my cells without lysing the nuclear membrane?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
What is the best way to stain cells with Membrane Marker 1-43 (MM 1-43)?