Name: Lumiwox™ acridinium NHS ester
Brand: AAT Bioquest
SKU: 26000
Price: 232 USD
Availability: InStock

Lumiwox™ acridinium NHS ester

The acridinium NHS ester can be used to label proteins and nucleic acids. The covalently bound acridinium NHS esters generate chemiluminescence in the presence of hydrogen peroxide. Exposure of an acridinium ester label to an alkaline hydrogen peroxide solution triggers a flash of light. Acridinium-labeled proteins and other biological molecules can be used as sensitive detection methods in immunoassays and other biological detections. Compared to the other commercial acridinium NHS esters, the conjugates prepared from Lumiwox™ acridinium NHS ester generate stronger luminescence signals. In addition, the conjugates derived from Lumiwox™ acridinium NHS ester still maintain their acridinium tags attached to their target molecules upon hydrogen peroxide-induced chemiluminescence reaction while the conjugates derived from the other commercial acridinium NHS esters lose their acridinium tags upon hydrogen peroxide-induced chemiluminescence reaction. This critical feature enables Lumiwox™ acridinium probe useful for biological detections on various surfaces, e.g., microarrays etc.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
26000	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Lumiwox™ acridinium NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	150.119 µL	750.593 µL	1.501 mL	7.506 mL	15.012 mL
5 mM	30.024 µL	150.119 µL	300.237 µL	1.501 mL	3.002 mL
10 mM	15.012 µL	75.059 µL	150.119 µL	750.593 µL	1.501 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	666.14 g/mol	=	mL	x	mM	=	mmol

Citations

View all 3 citations: Citation Explorer

Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification

Authors:

Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined

Journal:

Metabolomics (Los Angel) (2017): 2153--0769

Suramin inhibits cullin-RING E3 ubiquitin ligases

Authors:

Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined

Journal:

Proceedings of the National Academy of Sciences (2016): E2011--E2018

Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function

Authors:

Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined

Journal:

International Journal of Cancer (2012): E425--E436

Physical properties

Molecular weight	666.14
Solvent	DMSO

Storage, safety and handling

H-phrase	H301, H311, H331
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R23, R24, R25
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 12, 2025