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LysoBrite™ Deep Red
Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris. Lysosomes digest excess or worn-out organelles, food particles, and engulfed viruses or bacteria. The membrane around a lysosome allows the digestive enzymes to work at pH 4.5. The interior of the lysosomes is acidic (pH 4.5-4.8) compared to the slightly alkaline cytosol (pH 7.2). The lysosome maintains this pH differential by pumping protons from the cytosol across the membrane via proton pumps and chloride ion channels. LysoBrite™ Deep Red selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and trapped in lysosomes after it gets into cells. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly reduces its staining background and makes it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells. LysoBrite™ dyes significantly outperform the equivalent LysoTracker ™dyes (from Invitrogen). LysoBrite™ dyes can stay in live cells for more than a week with very minimal cell toxicity while the LysoTracker dyes can only be used for a few hours. LysoBrite™ dyes can survive a few generations of cell division. In addition, LysoBrite™ dyes are much more photostable than the LysoTracker dyes.
Image of Hela cells stained with the A: LysoBrite™ Deep Redor B: LysoTracker® Red DND-99 (from Invitrogen) in a Costar black 96-well plate. The TRTIC signals were compared at 0 and 120 seconds exposure time by using an Olympus fluorescence microscope.
Image of Hela cells stained with the A: LysoBrite™ Deep Redor B: LysoTracker® Red DND-99 (from Invitrogen) in a Costar black 96-well plate. The TRTIC signals were compared at 0 and 120 seconds exposure time by using an Olympus fluorescence microscope.
protocol
Protocol
sds
SDS
COO
COA
CatalogSize
Price
Quantity
22646500 Tests
Price
 
Physical properties
Molecular weight746.99
SolventDMSO
Spectral properties
Excitation (nm)597
Emission (nm)619
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation561 nm laser
Emission630/30 nm filter
Fluorescence microscope
ExcitationCy3/TRITC filter set
EmissionCy3/TRITC filter set
Recommended plateBlack wall/clear bottom
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Page updated on October 13, 2025