LysoBrite™ Green
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Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Molecular weight | 453.34 |
Solvent | DMSO |
Excitation (nm) | 501 |
Emission (nm) | 510 |
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
LysoBrite™ Red DND-99 |
LysoBrite™ Green DND-26 |
Overview | ![]() ![]() |
Molecular weight 453.34 | Excitation (nm) 501 | Emission (nm) 510 |
Platform
Flow cytometer
Excitation | 488 nm laser |
Emission | 530/30 nm filter |
Instrument specification(s) | FITC channel |
Fluorescence microscope
Excitation | FITC filter set |
Emission | FITC filter set |
Recommended plate | Black wall/clear bottom |
Fluorescence microplate reader
Excitation | 490 |
Emission | 525 |
Cutoff | 515 |
Recommended plate | Black wall/clear bottom |
Instrument specification(s) | Bottom read mode |
Example protocol
AT A GLANCE
- Prepare cells
Add dye working solution
Incubate at 37 °C for 30 minutes
- Wash the cells
Analyze under a fluorescence microscope
The LysoBrite™ Green stock solution provided is 500X in DMSO. It should be stable for at least 6 months if stored at -20°C. Protect from light, and avoid freeze/thaw cycles.
PREPARATION OF WORKING SOLUTION
Warm LysoBrite™ Green dye to room temperature.
Prepare dye working solution by diluting 20 µL of 500X LysoBrite™ Green with 10 mL of Hanks and 20 mM HEPES buffer (HBSS) or buffer of your choice.
Note: 20 µL of LysoBrite™ Green dye is enough for one 96-well plate. Aliquot and store unused LysoBrite™ dye stock solutions at < -15 °C. Protect it from light and avoid repeated freeze-thaw cycles.
Note: The optimal concentration of the fluorescent lysosome indicator varies depending on the specific application. The staining conditions may be modified according to the particular cell type and the permeability of the cells or tissues to the probe.
SAMPLE EXPERIMENTAL PROTOCOL
This protocol only provides a guideline and should be modified according to your specific needs.
Grow cells in a 96-well black wall/clear bottom plate (100 µL/well/96-well plate) or on coverslips inside a petri dish filled with the appropriate culture medium.
When cells reach the desired confluence, add an equal volume of the dye-working solution (from Preparation of Working Solution Step 2).
Incubate the cells in a 37 °C, 5% CO2 incubator for 30 minutes.
Wash the cells twice with pre-warmed (37 °C) Hanks and 20 mM HEPES buffer (HBSS) or buffer of your choice. Then fill the cell wells with HBSS or growth medium.
Observe the cells using a fluorescence microscope fitted with the desired filter set.
Note: It is recommended to increase either the labeling concentration or the incubation time to allow the dye to accumulate if the cells do not appear to be sufficiently stained.
Add an equal volume of the dye-working solution (from Preparation of Working Solution Step 2).
Incubate the cells in a 37 °C, 5% CO2 incubator for 30 minutes.
Wash the cells twice with pre-warmed (37 °C) Hanks and 20 mM HEPES buffer (HBSS) or buffer of your choice. Then fill the cell wells with HBSS or growth medium.
Observe the cells using a fluorescence microscope fitted with the desired filter set.
Note: It is recommended to increase either the labeling concentration or the incubation time to allow the dye to accumulate if the cells do not appear to be sufficiently stained.
Note: Suspension cells may be attached to coverslips treated with BD Cell-Tak® (BD Biosciences) and stained as adherent cells (see Protocol for Preparing and Staining Adherent Cells).
Calculators
Common stock solution preparation
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 220.585 µL | 1.103 mL | 2.206 mL | 11.029 mL | 22.058 mL |
5 mM | 44.117 µL | 220.585 µL | 441.17 µL | 2.206 mL | 4.412 mL |
10 mM | 22.058 µL | 110.292 µL | 220.585 µL | 1.103 mL | 2.206 mL |
Molarity calculator
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Product Family
Name | Excitation (nm) | Emission (nm) |
LysoBrite™ Blue | 434 | 480 |
LysoBrite™ Orange | 543 | 565 |
LysoBrite™ Red | 576 | 596 |
LysoBrite™ Deep Red | 597 | 619 |
LysoBrite™ NIR | 636 | 651 |
Images

Citations
Authors: Li, Fengqiao and Zhang, Xue-Qing and Ho, William and Tang, Maoping and Li, Zhongyu and Bu, Lei and Xu, Xiaoyang
Journal: Nature Communications (2023): 4223
Authors: Khan, Nabab and Halcrow, Peter W and Afghah, Zahra and Baral, Aparajita and Geiger, Jonathan D and Chen, Xuesong
Journal: The FASEB Journal (2022): e22184
Authors: Mao, Guobin and Wu, Guoqiang and Chen, Minghai and Yan, Chuang and Tang, Jingya and Ma, Yingxin and Zhang, Xian-En
Journal: Analytical Chemistry (2022): 6665--6671
Authors: Meng, Junli and Zhang, Peisen and Chen, Qizhe and Wang, Zihua and Gu, Yuan and Ma, Jie and Li, Wang and Yang, Chen and Qiao, Yuanyuan and Hou, Yi and others,
Journal: Advanced Materials (2022): 2202168
Authors: Imani, Rana and Prakash, Satya and Vali, Hojatollah and Presley, John F and Faghihi, Shahab
Journal: (2021)
Authors: Imani, Rana and Prakash, Satya and Vali, Hojatollah and Faghihi, Shahab
Journal: Biomaterials Science (2018)
Authors: Cemma, Marija and Grinstein, Sergio and Brumell, John H
Journal: Autophagy (2016): 1440--1446
Authors: Myung, Ja Hye and Gajjar, Khyati A and Chen, Jihua and Molokie, Robert E and Hong, Seungpyo
Journal: Analytical chemistry (2014): 6088--6094
Authors: Lei, Bo and Chen, Xiaofeng and Han, Xue and Zhou, Jiaan
Journal: Journal of Materials Chemistry (2012): 16906--16913
Authors: Liu, Rongjun and Zhang, Liangliang and Zhao, Jingjin and Luo, Zhihui and Huang, Yong and Zhao, Shulin
Journal: Advanced Therapeutics : 1800041
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